AB183716

重组Anti-NDUFB11抗体[EPR15252]

Anti-NDUFB11 antibody [EPR15252]

RabMAb
Recombinant
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(13 Publications)

Rabbit Recombinant Monoclonal NDUFB11 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 13 publications.

查看别名

UNQ111/PRO1064, NDUFB11, Complex I-ESSS, NADH-ubiquinone oxidoreductase ESSS subunit, Neuronal protein 17.3, CI-ESSS, Np17.3, p17.3

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB11 antibody [EPR15252] (AB183716)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB11 antibody [EPR15252] (AB183716)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NDUFB11 with ab183716 at a 1/250 dilution. Counter stained with hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-NDUFB11 antibody [EPR15252] (AB183716)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-NDUFB11 antibody [EPR15252] (AB183716)

Intracellular Flow Cytometry analysis of paraformaldehyde-fixed HeLa cells labeling NDUFB11 with ab183716 at a 1/240 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, or negative control rabbit IgG (green).

Supplier Data

Western blot - Anti-NDUFB11 antibody [EPR15252] (AB183716)

Lanes 1 - 2:

Western blot - Anti-NDUFB11 antibody [EPR15252] (ab183716) at 1/50000 dilution

Lane 3:

Western blot - Anti-NDUFB11 antibody [EPR15252] (ab183716) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Lane 3:

A431 cell lysate at 20 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 17 kDa

Observed band size: 17 kDa

true

Supplier Data

Western blot - Anti-NDUFB11 antibody [EPR15252] (AB183716)

All lanes:

Western blot - Anti-NDUFB11 antibody [EPR15252] (ab183716) at 1/10000 dilution

All lanes:

Human heart tissue lysate at 20 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 17 kDa

Observed band size: 17 kDa

true

不同偶联物与剂型 (1)

Carrier free

Anti-NDUFB11 antibody [EPR15252] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR15252

亚型

IgG

不含载体蛋白

反应种属

Human

应用

Flow Cyt (Intra), WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/240", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

NDUFB11 also known as NADH:ubiquinone oxidoreductase subunit B11 is a component of mitochondrial Complex I. It has a molecular weight of approximately 12 kDa. This protein contributes to the proper function of the electron transport chain. NDUFB11 is expressed mainly in tissues with high energy demands like the heart muscle and brain. It plays a role in facilitating electron transfer from NADH to ubiquinone which is a critical step in cellular respiration.

Biological function summary

NDUFB11 becomes part of the mitochondrial respiratory chain complex I an essential structure for ATP production through oxidative phosphorylation. This complex includes multiple subunits and is located on the inner mitochondrial membrane. NDUFB11 contributes to the assembly and stability of this complex ensuring efficient energy production within cells. Complex I dysfunction often impacts energy-dependent tissues leading to various metabolic challenges.

Pathways

NDUFB11 participates in the oxidative phosphorylation pathway vital for energy production in eukaryotic cells. It interacts with other important proteins of the electron transport chain like NDUFS1 and NDUFA13. These proteins collectively ensure the smooth transfer of electrons for ATP synthesis. Additionally NDUFB11 is involved in the mitochondrial electron transport which is essential for maintaining cellular metabolic processes.

NDUFB11 mutations link to mitochondrial diseases such as Leigh syndrome characterized by neurometabolic dysfunction. This protein's malfunction can also connect to complex I deficiency affecting cellular respiration and leading to various clinical symptoms including muscle weakness and neurological impairments. Mitochondrial diseases associated with NDUFB11 may also involve interactions with other mitochondrial subunits indicating an intricate relationship with overall mitochondrial health.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

See full target information NDUFB11

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文献 (13)

Recent publications for all applications. Explore the full list and refine your search

Molecular biology of the cell 36:ar33 PubMed39878654

2025

Adaptive protein synthesis in genetic models of copper deficiency and childhood neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Alicia R Lane,Noah E Scher,Shatabdi Bhattacharjee,Stephanie A Zlatic,Anne M Roberts,Avanti Gokhale,Kaela S Singleton,Duc M Duong,Mike McKenna,William L Liu,Alina Baiju,Felix G Rivera Moctezuma,Tommy Tran,Atit A Patel,Lauren B Clayton,Michael J Petris,Levi B Wood,Anupam Patgiri,Alysia D Vrailas-Mortimer,Daniel N Cox,Blaine R Roberts,Erica Werner,Victor Faundez

Journal of translational medicine 22:431 PubMed38715059

2024

Multiomics analysis identifies oxidative phosphorylation as a cancer vulnerability arising from myristoylation inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Erwan Beauchamp,Jay M Gamma,Christopher R Cromwell,Eman W Moussa,Rony Pain,Morris A Kostiuk,Claudia Acevedo-Morantes,Aishwarya Iyer,Megan Yap,Krista M Vincent,Lynne M Postovit,Olivier Julien,Basil P Hubbard,John R Mackey,Luc G Berthiaume

Biochemical pharmacology 218:115925 PubMed37981173

2023

A novel gene signature to diagnose MASLD in metabolically unhealthy obese individuals.

Applications

Unspecified application

Species

Unspecified reactive species

Marica Meroni,Federica Chiappori,Erika Paolini,Miriam Longo,Emilia De Caro,Ettore Mosca,Alice Chiodi,Ivan Merelli,Sara Badiali,Marco Maggioni,Alessandra Mezzelani,Luca Valenti,Anna Ludovica Fracanzani,Paola Dongiovanni

Cardiovascular research 119:2213-2229 PubMed37395010

2023

Tissue-specific differences in the assembly of mitochondrial Complex I are revealed by a novel ENU mutation in ECSIT.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Nicol,Sara Falcone,Andrew Blease,Pratik Vikhe,Gabriele Civiletto,Saleh Salman Omairi,Carlo Viscomi,Ketan Patel,Paul K Potter

eLife 11: PubMed36255054

2022

Tissue-specific mitochondrial HIGD1C promotes oxygen sensitivity in carotid body chemoreceptors.

Applications

Unspecified application

Species

Unspecified reactive species

Alba Timón-Gómez,Alexandra L Scharr,Nicholas Y Wong,Erwin Ni,Arijit Roy,Min Liu,Julisia Chau,Jack L Lampert,Homza Hireed,Noah S Kim,Masood Jan,Alexander R Gupta,Ryan W Day,James M Gardner,Richard J A Wilson,Antoni Barrientos,Andy J Chang

Cell metabolism 33:531-546.e9 PubMed33545050

2021

C9orf72 regulates energy homeostasis by stabilizing mitochondrial complex I assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Wang,Honghe Liu,Kie Itoh,Sungtaek Oh,Liang Zhao,Daisuke Murata,Hiromi Sesaki,Thomas Hartung,Chan Hyun Na,Jiou Wang

Nature communications 11:5469 PubMed33122634

2020

Intellectual disability-associated factor Zbtb11 cooperates with NRF-2/GABP to control mitochondrial function.

Applications

Unspecified application

Species

Unspecified reactive species

Brooke C Wilson,Lena Boehme,Ambra Annibali,Alan Hodgkinson,Thomas S Carroll,Rebecca J Oakey,Vlad C Seitan

STAR protocols 1: PubMed32995753

2020

Protocol for the Analysis of Yeast and Human Mitochondrial Respiratory Chain Complexes and Supercomplexes by Blue Native Electrophoresis.

Applications

Unspecified application

Species

Unspecified reactive species

Alba Timón-Gómez,Rafael Pérez-Pérez,Eva Nyvltova,Cristina Ugalde,Flavia Fontanesi,Antoni Barrientos

Cell reports 31:107607 PubMed32375044

2020

Distinct Roles of Mitochondrial HIGD1A and HIGD2A in Respiratory Complex and Supercomplex Biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Alba Timón-Gómez,Joshua Garlich,Rosemary A Stuart,Cristina Ugalde,Antoni Barrientos

Nature communications 11:1643 PubMed32242014

2020

A salvage pathway maintains highly functional respiratory complex I.

Applications

Unspecified application

Species

Unspecified reactive species

Karolina Szczepanowska,Katharina Senft,Juliana Heidler,Marija Herholz,Alexandra Kukat,Michaela Nicole Höhne,Eduard Hofsetz,Christina Becker,Sophie Kaspar,Heiko Giese,Klaus Zwicker,Sergio Guerrero-Castillo,Linda Baumann,Johanna Kauppila,Anastasia Rumyantseva,Stefan Müller,Christian K Frese,Ulrich Brandt,Jan Riemer,Ilka Wittig,Aleksandra Trifunovic

View all publications

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重组Anti-NDUFB11抗体[EPR15252]