Anti-NDUFA4L2抗体(ab74138)
Key features and details
- Rabbit polyclonal to NDUFA4L2
- Suitable for: WB, ELISA
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-NDUFA4L2抗体 -
描述
兔多克隆抗体to NDUFA4L2 -
宿主
Rabbit -
经测试应用
适用于: WB, ELISAmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide derived from an internal sequence of human NDUFA4L2.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab74138于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Detects a band of approximately <19 kDa (predicted molecular weight: 10 kDa).
|
| ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately <19 kDa (predicted molecular weight: 10 kDa). |
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ELISA
Use at an assay dependent concentration. |
靶标
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序列相似性
Belongs to the complex I NDUFA4 subunit family. - Information by UniProt
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数据库链接
- Entrez Gene: 56901 Human
- Entrez Gene: 407790 Mouse
- SwissProt: Q9NRX3 Human
- SwissProt: Q4FZG9 Mouse
- Unigene: 75069 Human
- Unigene: 336856 Mouse
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别名
- FLJ26118 antibody
- NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 4 like 2 antibody
- NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4-like 2 antibody
see all
图片
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Western blot - Anti-NDUFA4L2 antibody (ab74138)All lanes : Anti-NDUFA4L2 antibody (ab74138) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells
Lane 2 : Extracts from COLO cells
Lane 3 : Extracts from HUVEC cells
Lane 4 : Extracts from HepG2 cells
Lane 5 : Extracts from HepG2 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 10 kDa
Observed band size: <19 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab74138 被引用在 5 文献中.
- Kubala JM et al. NDUFA4L2 reduces mitochondrial respiration resulting in defective lysosomal trafficking in clear cell renal cell carcinoma. Cancer Biol Ther 24:2170669 (2023). PubMed: 36722045
- Zheng J et al. Induction of the mitochondrial NDUFA4L2 protein by HIF-1a regulates heart regeneration by promoting the survival of cardiac stem cell. Biochem Biophys Res Commun N/A:N/A (2018). WB ; Rat . PubMed: 29953852
- Tahrir FG et al. Dysregulation of mitochondrial bioenergetics and quality control by HIV-1 Tat in cardiomyocytes. J Cell Physiol 233:748-758 (2018). PubMed: 28493473
- Miller BA et al. TRPM2 channels protect against cardiac ischemia-reperfusion injury: role of mitochondria. J Biol Chem 289:7615-29 (2014). WB ; Mouse . PubMed: 24492610
- Chen SJ et al. A splice variant of the human ion channel TRPM2 modulates neuroblastoma tumor growth through hypoxia-inducible factor (HIF)-1/2a. J Biol Chem 289:36284-302 (2014). WB . PubMed: 25391657