重组Anti-NDUFA1抗体[EPR12083] (ab176563)
![Western blot - Anti-NDUFA1 antibody [EPR12083] (ab176563)](https://www.abcam.cn/ps/products/176/ab176563/Images/ab176563-204265-anti-ndufa1-antibody-epr12083-western-blot.jpg "Western blot - Anti-NDUFA1 antibody [EPR12083] (ab176563)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12083] to NDUFA1
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-NDUFA1抗体[EPR12083]
参阅全部 NDUFA1 一抗 -
描述
兔单克隆抗体[EPR12083] to NDUFA1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, IPmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Human
预测可用于: Gorilla
不与反应:
Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- A673 cell lysate; Human skeletal muscle, Human fetal kidney and fetal heart lysates; Human heart and skeletal muscle tissues.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR12083 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab176563于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 8 kDa.
|
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| IP |
1/10 - 1/100.
|
| 说明 |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 8 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
IP
1/10 - 1/100. |
靶标
-
功能
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. -
组织特异性
Primarily expressed in heart and skeletal muscle. -
疾病相关
Defects in NDUFA1 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. -
序列相似性
Belongs to the complex I NDUFA1 subunit family. -
细胞定位
Mitochondrion inner membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 101144612 Gorilla
- Entrez Gene: 4694 Human
- Omim: 300078 Human
- SwissProt: Q8SPF0 Gorilla
- SwissProt: O15239 Human
- Unigene: 534168 Human
-
别名
- CI MWFE antibody
- CI-MWFE antibody
- Complex I MWFE antibody
see all
图片
-
Western blot - Anti-NDUFA1 antibody [EPR12083] (ab176563)All lanes : Anti-NDUFA1 antibody [EPR12083] (ab176563) at 1/1000 dilution
Lane 1 : A673 cell lysate
Lane 2 : Human fetal kidney tissue lysate
Lane 3 : Human skeletal muscle tissue lysate
Lane 4 : Human fetal heart tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 8 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFA1 antibody [EPR12083] (ab176563)](https://www.abcam.cn/ps/products/176/ab176563/Images/ab176563-204262-anti-ndufa1-antibody-epr12083-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFA1 antibody [EPR12083] (ab176563)Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human heart tissue labeling NDUFA1 with ab176563 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFA1 antibody [EPR12083] (ab176563)](https://www.abcam.cn/ps/products/176/ab176563/Images/ab176563-204261-anti-ndufa1-antibody-epr12083-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFA1 antibody [EPR12083] (ab176563)Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human skeletal muscle tissue labeling NDUFA1 with ab176563 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunoprecipitation - Anti-NDUFA1 antibody [EPR12083] (ab176563)](https://www.abcam.cn/ps/products/176/ab176563/Images/ab176563-204260-anti-ndufa1-antibody-epr12083-immunoprecipitation.jpg)
Immunoprecipitation - Anti-NDUFA1 antibody [EPR12083] (ab176563)Western blot analysis on immunoprecipitation pellet from Human fetal heart lysate labeling NDUFA1 with ab176563 at 1/10 dilution.
-
![Anti-NDUFA1 antibody [EPR12083] (ab176563)](https://www.abcam.cn/ps/products/176/ab176563/Images/ab176563-5-benefits-of-recombinant-antibodies.png)
Anti-NDUFA1 antibody [EPR12083] (ab176563)
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab176563 被引用在 2 文献中.
- Sen T et al. Decreased PGC1ß expression results in disrupted human erythroid differentiation, impaired hemoglobinization and cell cycle exit. Sci Rep 11:17129 (2021). PubMed: 34429458
- Jing YY et al. Epigenetic regulation of the Warburg effect by H2B monoubiquitination. Cell Death Differ 27:1660-1676 (2020). PubMed: 31685978