Anti-NDUFA1抗体(ab131423)
Key features and details
- Rabbit polyclonal to NDUFA1
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-NDUFA1抗体
参阅全部 NDUFA1 一抗 -
描述
兔多克隆抗体to NDUFA1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Rat, Human -
免疫原
Synthetic peptide corresponding to a sequence at the C terminal of Human NDUFA1, different from the related rat and mouse sequences by one amino acid.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.1% Dibasic monohydrogen sodium phosphate, 0.45% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131423于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 8 kDa.
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| IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| 说明 |
|---|
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WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 8 kDa. |
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IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. -
组织特异性
Primarily expressed in heart and skeletal muscle. -
疾病相关
Defects in NDUFA1 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. -
序列相似性
Belongs to the complex I NDUFA1 subunit family. -
细胞定位
Mitochondrion inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 4694 Human
- Entrez Gene: 363441 Rat
- Omim: 300078 Human
- SwissProt: O15239 Human
- Unigene: 534168 Human
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别名
- CI MWFE antibody
- CI-MWFE antibody
- Complex I MWFE antibody
see all
图片
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Western blot - Anti-NDUFA1 antibody (ab131423)All lanes : Anti-NDUFA1 antibody (ab131423) at 0.5 µg/ml
Lane 1 : Rat Heart tissue lysate
Lane 2 : Rat Brain tissue lysate
Lane 3 : Rat Liver tissue lysate
Lane 4 : Rat Kidney tissue lysate
Lane 5 : HT1080 Cell Lysate
Predicted band size: 8 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFA1 antibody (ab131423)Immunohistochemical analysis of paraffin-embedded Rat Cardiac Muscle Tissue labelling NDUFA1 with ab131423 at 1µg/ml.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab131423 被引用在 2 文献中.
- Mulcahy MJ et al. Brain Region-Specific nAChR and Associated Protein Abundance Alterations Following Chronic Nicotine and/or Menthol Exposure. J Proteome Res 19:36-48 (2020). PubMed: 31657575
- Wu S et al. The circ_0004463/miR-380-3p/FOXO1 axis modulates mitochondrial respiration and bladder cancer cell apoptosis. Cell Cycle 19:3563-3580 (2020). PubMed: 33283616