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重组RabMAb

重组Anti-NDRG3抗体[EPR9010(B)] (ab131266)

Submit a review Submit a question References (3)
Western blot - Anti-NDRG3 antibody [EPR9010(B)] (ab131266)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG3 antibody [EPR9010(B)] (ab131266)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG3 antibody [EPR9010(B)] (ab131266)
  • OI-RD Scanning - Anti-NDRG3 antibody [EPR9010(B)] (ab131266)
  • Anti-NDRG3 antibody [EPR9010(B)] (ab131266)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9010(B)] to NDRG3
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-NDRG3抗体[EPR9010(B)]
    参阅全部 NDRG3 一抗

  • 描述

    兔单克隆抗体[EPR9010(B)] to NDRG3

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-Pmore details
    不适用于: IP

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • Raji, Human fetal brain and LNCaP lysates; Human kidney and brain tissue

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • 解离常数（KD）

    KD = 1.92 x 10 -11 M
    Learn more about KD

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR9010(B)

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab131266于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 41 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
说明
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 41 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
应用说明
Is unsuitable for IP.

靶标

图片

  • Western blot - Anti-NDRG3 antibody [EPR9010(B)] (ab131266)
    Western blot - Anti-NDRG3 antibody [EPR9010(B)] (ab131266)
    All lanes : Anti-NDRG3 antibody [EPR9010(B)] (ab131266) at 1/1000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : Human fetal brain tissue lysate
    Lane 3 : LNCaP cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 41 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG3 antibody [EPR9010(B)] (ab131266)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG3 antibody [EPR9010(B)] (ab131266)

    Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling NDRG3 with ab131266 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG3 antibody [EPR9010(B)] (ab131266)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG3 antibody [EPR9010(B)] (ab131266)

    Immunohistochemical analysis of paraffin embedded Human brain tissue labelling NDRG3 with ab131266 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-NDRG3 antibody [EPR9010(B)] (ab131266)
    OI-RD Scanning - Anti-NDRG3 antibody [EPR9010(B)] (ab131266)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-NDRG3 antibody [EPR9010(B)] (ab131266)
    Anti-NDRG3 antibody [EPR9010(B)] (ab131266)

文献 (3)

发表研究结果有使用 ab131266？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab131266 被引用在 3 文献中.

  • Luo Z  et al. LncRNA Meg3 promotes oxygen and glucose deprivation injury by decreasing angiogenesis in hBMECs by targeting the miR‑122‑5p/NDRG3 axis. Exp Ther Med 24:622 (2022). PubMed: 36160904
  • Lee JC  et al. The Iron Chelator, Dp44mT, Effectively Inhibits Human Oral Squamous Cell Carcinoma Cell Growth in Vitro and in Vivo. Int J Mol Sci 17:N/A (2016). PubMed: 27589737
  • Tsui KH  et al. Growth differentiation factor-15: a p53- and demethylation-upregulating gene represses cell proliferation, invasion, and tumorigenesis in bladder carcinoma cells. Sci Rep 5:12870 (2015). WB . PubMed: 26249737

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