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使用敲除细胞株进行验证重组RabMAb

重组Anti-NDRG1抗体[EPR5593] - BSA and Azide free (ab226082)

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Flow Cytometry (Intracellular) - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)
  • Immunocytochemistry/ Immunofluorescence - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)
  • Western blot - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)
  • Immunoprecipitation - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)
  • Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5593] to NDRG1 - BSA and Azide free
  • Suitable for: WB, IP, IHC-P, ICC/IF, Flow Cyt (Intra)
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Unconjugated

概述

  • 产品名称

    Anti-NDRG1抗体[EPR5593] - BSA and Azide free
    参阅全部 NDRG1 一抗

  • 描述

    兔单克隆抗体[EPR5593] to NDRG1 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IP, IHC-P, ICC/IF, Flow Cyt (Intra)more details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IHC-P: Human colon tissue, human liver carcinoma tissue, Mouse and rat colon tissue. ICC/IF: Jurkat (Human T cell leukemia T lymphocyte) cells. IP: HeLa. WB: Wild-type HEK-293 whole cell lysate. Jurkat, HeLa, Caco-2 and LnCap whole cell lysate. Mouse and rat brain lysate. Flow cyto(intra): HeLa (Human cervix adenocarcinoma epithelial cell)

  • 常规说明

    ab226082 is the carrier-free version of ab124689.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab226082于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration. Detects a band of approximately 48 kDa (predicted molecular weight: 43 kDa).
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
说明
WB
Use at an assay dependent concentration. Detects a band of approximately 48 kDa (predicted molecular weight: 43 kDa).
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.

靶标

  • 功能

    May have a growth inhibitory role.

  • 组织特异性

    Ubiquitous; expressed most prominently in placental membranes and prostate, kidney, small intestine, and ovary tissues. Reduced expression in adenocarcinomas compared to normal tissues. In colon, prostate and placental membranes, the cells that border the lumen show the highest expression.

  • 疾病相关

    Defects in NDRG1 are the cause of Charcot-Marie-Tooth disease type 4D (CMT4D) [MIM:601455]; also known as hereditary motor and sensory neuropathy Lom type (HMSNL). CMT4D is a recessive form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy and primary peripheral axonal neuropathy. Demyelinating CMT neuropathies are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. By convention, autosomal recessive forms of demyelinating Charcot-Marie-Tooth disease are designated CMT4.

  • 序列相似性

    Belongs to the NDRG family.

  • 细胞定位

    Cytoplasm. Nucleus. Cell membrane. Whereas in prostate epithelium and placental chorion it is located in both the cytoplasm and the nucleus, nuclear staining is not observed in colon epithelium cells. Instead its localization changes from the cytoplasm to the plasma membrane during differentiation of colon carcinoma cell lines in vitro.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • 42 kDa antibody
    • Anti GC4 antibody
    • cap43 antibody
    • cmt4d antibody
    • Differentiation related gene1 protein antibody
    • Differentiation-related gene 1 protein antibody
    • Drg 1 antibody
    • DRG-1 antibody
    • drg1 antibody
    • gc4 antibody
    • GC4 antibody antibody
    • hmsnl antibody
    • Human mRNA for RTP complete cds antibody
    • N myc downstream regulated gene 1 antibody
    • N myc downstream regulated gene 1 protein antibody
    • N-myc downstream-regulated gene 1 protein antibody
    • Ndr 1 antibody
    • ndr1 antibody
    • NDRG 1 antibody
    • Ndrg1 antibody
    • NDRG1 protein antibody
    • NDRG1_HUMAN antibody
    • Nickel specific induction protein antibody
    • Nickel specific induction protein Cap43 antibody
    • Nickel-specific induction protein Cap43 antibody
    • nmsl antibody
    • Nmyc downstream regulated antibody
    • Nmyc downstream regulated gene1 antibody
    • Nmyc downstream regulated gene1 protein antibody
    • Protein NDRG1 antibody
    • Protein regulated by oxygen 1 antibody
    • Protein regulated by oxygen1 antibody
    • Proxy1 antibody
    • Reduced in tumor antibody
    • Reducin antibody
    • Reducing agents and tunicamycin responsive protein antibody
    • Reducing agents and tunicamycin-responsive protein antibody
    • Rit42 antibody
    • RTP antibody
    • targ1 antibody
    • TDD5 antibody
    • tdds antibody
    • Tunicamycin responsive protein antibody
    see all

图片

  • Flow Cytometry (Intracellular) - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)
    Flow Cytometry (Intracellular) - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)

    This data was developed using ab124689, the same antibody clone in a different buffer formulation.

    Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling NDRG1 with purified ab124689 at 1/20 dilution (5 ug/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as a isotype control. Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

  • Immunocytochemistry/ Immunofluorescence - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)
    Immunocytochemistry/ Immunofluorescence - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)

    This data was developed using ab124689, the same antibody clone in a different buffer formulation.

    Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling NDRG1 using ab124689. The cells were fixed with 100% Methanol then permeabilized with 0.1% Triton X-100. The cells were then incubated with ab124689 at 1:50 dilution followed by a further incubation with a Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI. Cells were counterstained using ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1:200 dilution (shown in red). Secondary antibody only control: PBS instead of the primary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)

    This data was developed using ab124689, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of Paraffin-embedded sections mouse colon tissue labelling NDRG1 with ab124689 at 1/1000 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on mouse colon tissue is observed. Counter stained with Haematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)

    This data was developed using ab124689, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of Paraffin-embedded sections rat colon tissue labelling NDRG1 with ab124689 at 1/1000 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on rat colon tissue is observed. Counter stained with Haematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124689).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)

    This data was developed using ab124689, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of Paraffin-embedded sections human colon tissue labelling NDRG1 with ab124689 at 1/1000 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on human colon tissue is observed. Counter stained with Haematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Western blot - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)
    Western blot - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)
    All lanes : Anti-NDRG1 antibody [EPR5593] (ab124689) at 1/10000 dilution

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 43 kDa
    Observed band size: 48 kDa why is the actual band size different from the predicted?



    This data was developed using ab124689, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    ab181602 was used as GAPDH loading control.

  • Immunoprecipitation - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)
    Immunoprecipitation - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)

    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg.

    Lane 2 (+): ab124689 & HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124689 in HeLa whole cell lysate

    ab124689 (Purified) at 1/50 dilution (20µg/ml) immunoprecipitating NDRG1 in HeLa whole cell lysate. For western blotting, ab124689 at 1/500 dilution (1.86 µg/mL) and VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.

    Blocking and diluting buffer: 5% NFDM /TBST .

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124689).

  • Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)
    Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (0)

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Immunohistochemistry (Frozen sections) abreview for Anti-NDRG1 antibody [EPR5593] - BSA and Azide free

 Average
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Duodenum)
Permeabilization
Yes - 0.25% TritonX-100
Specification
Duodenum
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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提交于 Jul 26 2022

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