Anti-NDEL1抗体(ab25959)
Key features and details
- Rabbit polyclonal to NDEL1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-NDEL1抗体
参阅全部 NDEL1 一抗 -
描述
兔多克隆抗体to NDEL1 -
宿主
Rabbit -
特异性
ab25959 recognises NDEL1.
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经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human NDEL1.
(Peptide available asab39840) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab25959于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) |
Use a concentration of 2 µg/ml.
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| WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 38 kDa.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 2 µg/ml. |
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WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 38 kDa. |
靶标
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功能
Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth. Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL. -
组织特异性
Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle. -
序列相似性
Belongs to the nudE family. -
发展阶段
Expression peaks in mitosis. -
翻译后修饰
Phosphorylated in mitosis. Can be phosphorylated by CDK1, CDK5 and MAPK1. Phosphorylation by CDK5 promotes interaction with KATNA1 and YWHAE. -
细胞定位
Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Localizes to the cell body of the motor neurons and colocalizes with assembled neurofilaments within axonal processes. Localizes to the microtubules of the manchette in elongated spermatids. Colocalizes with DISC1 in the perinuclear region, including the centrosome (By similarity). Localizes to the interphase centrosome and the mitotic spindle. Localizes to the kinetochore in a CENPF-dependent manner. - Information by UniProt
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数据库链接
- Entrez Gene: 81565 Human
- Omim: 607538 Human
- SwissProt: Q9GZM8 Human
- Unigene: 372123 Human
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别名
- A. nidulans antibody
- DKFZp451M0318 antibody
- ENDOOLIGOPEPTIDASE A antibody
see all
图片
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Western blot - Anti-NDEL1 antibody (ab25959)Lane 1 : Anti-NDEL1 antibody (ab25959) at 0.5 µg/ml
Lane 2 : Anti-NDEL1 antibody (ab25959) at 1 µg/ml
Lane 3 : Anti-NDEL1 antibody (ab25959) at 2 µg/ml
All lanes : Jurkat whole cell lysate.
Predicted band size: 38 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-NDEL1 antibody (ab25959)ab25959 at 2µg/ml staining NDEL1 in Jurkat cells by ICC/IF
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Immunocytochemistry/ Immunofluorescence - Anti-NDEL1 antibody (ab25959)Immunofluorescence of NDEL1 in Jurkat cells using ab25959 at 10 ug/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab25959 被引用在 7 文献中.
- Rasoulouniriana D et al. T Cells Expressing a Modified FcγRI Exert Antibody-Dependent Cytotoxicity and Overcome the Limitations of CAR T-cell Therapy against Solid Tumors. Cancer Immunol Res 11:792-809 (2023). PubMed: 37070661
- Chapman DE et al. Regulation of in vivo dynein force production by CDK5 and 14-3-3e and KIAA0528. Nat Commun 10:228 (2019). PubMed: 30651536
- Turgay Y et al. SUN proteins facilitate the removal of membranes from chromatin during nuclear envelope breakdown. J Cell Biol 204:1099-109 (2014). Human . PubMed: 24662567
- Pei Z et al. The expression and roles of Nde1 and Ndel1 in the adult mammalian central nervous system. Neuroscience 271:119-36 (2014). PubMed: 24785679
- Leliveld SR et al. Insolubility of disrupted-in-schizophrenia 1 disrupts oligomer-dependent interactions with nuclear distribution element 1 and is associated with sporadic mental disease. J Neurosci 28:3839-45 (2008). PubMed: 18400883
- Alkam T et al. The extensive nitration of neurofilament light chain in the hippocampus is associated with the cognitive impairment induced by amyloid beta in mice. J Pharmacol Exp Ther : (2008). WB ; Mouse . PubMed: 18621989
- Li W et al. Specific developmental disruption of disrupted-in-schizophrenia-1 function results in schizophrenia-related phenotypes in mice. Proc Natl Acad Sci U S A 104:18280-5 (2007). IP ; Mouse . PubMed: 17984054