为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Signal Transduction Signaling Pathway Calcium Signaling Calcium Channels

Anti-NCX1抗体[C2C12] (ab2869)

  • Datasheet
  • SDS
Reviews (4)Q&A (2)References (28)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Immunohistochemistry (Frozen sections) - Anti-NCX1 antibody [C2C12] (ab2869)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCX1 antibody [C2C12] (ab2869)
  • Flow Cytometry - Anti-NCX1 antibody [C2C12] (ab2869)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCX1 antibody [C2C12] (ab2869)

Key features and details

  • Mouse monoclonal [C2C12] to NCX1
  • Suitable for: IHC-P, IHC-Fr, Flow Cyt
  • Reacts with: Human
  • Isotype: IgM

你可能会感兴趣

分析检测
Product image
Resazurin Assay Kit - Cell Cytotoxicity (Fluorometric) (ab112119)
一抗
Product image
Anti-BIN1 antibody [EPR13463-25] (ab185950)
一抗
Product image
Anti-Phospholamban antibody (ab85146)

查看更多相关产品

概述

  • 产品名称

    Anti-NCX1抗体[C2C12]
    参阅全部 NCX1 一抗
  • 描述

    小鼠单克隆抗体[C2C12] to NCX1
  • 宿主

    Mouse
  • 经测试应用

    适用于: IHC-P, IHC-Fr, Flow Cytmore details
  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Guinea pig, Dog, Pig
  • 免疫原

    Full length native protein (purified) corresponding to Dog NCX1. Purified from canine cardiac sodium/calcium exchanger.

  • 表位

    This antibody recognizes an epitope between amino acids 371-525 on the intracellular side of the plasma membrane.
  • 阳性对照

    • IHC-P: Normal human kidney tissue. IHC-Fr: Normal human kidney tissue
  • 常规说明

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • Primary antibody说明

    The sodium/calcium exchanger of cardiac sarcolemma rapidly transports calcium during excitation-contraction coupling and is the dominant myocardial calcium efflux mechanism. The sodium/calcium exchanger uses the transmembrane sodium gradient to catalyze countertransport of calcium against its electrochemical gradient in a 3 sodium : 1 calcium exchange. Sodium/calcium exchange activity is present in excitable cells and in non-excitable cells.
  • 克隆

    单克隆
  • 克隆编号

    C2C12
  • 同种型

    IgM
  • 研究领域

    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Channels
    • Cardiovascular
    • Heart
    • Contractility
    • Inotropics
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG+IgM H&L (HRP) preadsorbed (ab47827)
    • Goat Anti-Mouse IgM mu chain (DyLight® 488) (ab97007)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2869于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P (2)
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr (1)
Use a concentration of 1 µg/ml.

PubMed: 21408028

Flow Cyt
1/20 - 1/100.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

说明
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr
Use a concentration of 1 µg/ml.

PubMed: 21408028

Flow Cyt
1/20 - 1/100.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

靶标

  • 功能

    Rapidly transports Ca(2+) during excitation-contraction coupling. Ca(2+) is extruded from the cell during relaxation so as to prevent overloading of intracellular stores.
  • 组织特异性

    Expressed in cardiac sarcolemma, brain, kidney, liver, pancreas, skeletal muscle, placenta and lung.
  • 序列相似性

    Belongs to the sodium/potassium/calcium exchanger family. SLC8 subfamily.
    Contains 2 Calx-beta domains.
  • 细胞定位

    Cell membrane.
  • Target information above from: UniProt accession P32418 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 6546 Human
    • Entrez Gene: 20541 Mouse
    • Entrez Gene: 397365 Pig
    • Entrez Gene: 100328570 Rabbit
    • Entrez Gene: 29715 Rat
    • Omim: 182305 Human
    • SwissProt: P23685 Dog
    • SwissProt: P48766 Guinea pig
    • SwissProt: P32418 Human
    • SwissProt: P70414 Mouse
    • SwissProt: Q01728 Rat
    • Unigene: 31961 Human
    • Unigene: 468274 Human
    • Unigene: 728911 Human
    • Unigene: 265834 Mouse
    • Unigene: 118972 Rat
    see all
  • 别名

    • CNC antibody
    • DKFZp779F0871 antibody
    • MGC119581 antibody
    • Na(+)/Ca(2+)-exchange protein 1 antibody
    • Na+/Ca2+ exchange protein 1 antibody
    • Na+/Ca2+ exchanger antibody
    • NAC1_HUMAN antibody
    • NCX 1 antibody
    • NCX antibody
    • NCX1 antibody
    • SLC8A1 antibody
    • SLC8A1 protein antibody
    • Sodium Calcium Exchanger antibody
    • Sodium/calcium exchanger 1 antibody
    • Solute carrier family 8 (sodium/calcium exchanger) member 1 antibody
    • Solute carrier family 8 member 1 antibody
    see all

图片

  • Immunohistochemistry (Frozen sections) - Anti-NCX1 antibody [C2C12] (ab2869)
    Immunohistochemistry (Frozen sections) - Anti-NCX1 antibody [C2C12] (ab2869)

    IHC image of NCX1 staining in a section of frozen normal human kidney*. The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature.  The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab2869 at 1µg/ml and ab6046 (Rabbit polyclonal to beta Tubulin - Loading Control) at 1/1000. The section was then incubated with ab150117 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488), 1/1000)) (shown in green) and ab150080 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594), 1/1000) (shown in red) for 1 hour at room temperature. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. The secondary-only control insert image is taken from an identical assay without primary antibody. The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times. *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCX1 antibody [C2C12] (ab2869)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCX1 antibody [C2C12] (ab2869)

    IHC image of NCX1 staining in a section of formalin-fixed paraffin-embedded normal human kidney* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab2869, 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Flow Cytometry - Anti-NCX1 antibody [C2C12] (ab2869)
    Flow Cytometry - Anti-NCX1 antibody [C2C12] (ab2869)

    Overlay histogram showing HEK293 cells stained with ab2869 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2869, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCX1 antibody [C2C12] (ab2869)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCX1 antibody [C2C12] (ab2869)This image is courtesy of an anonymous Abreview
    ab2869 staining NCX1 in Human kidney tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.05% Tween20 and blocked with 5% normal goat serum in 1XPBS + 0.05% Tween20 for 1 hour at 25°C; antigen retrieval was by heat mediation in sodium citrate (pH 6.0) buffer. Samples were incubated with primary antibody (1/100 in blocking buffer) for 1 hour at 25°C. Ab47827 (1/500) was used as the secondary antibody.

    See Abreview

实验方案

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (28)

发表研究结果有使用 ab2869?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab2869 被引用在 28 文献中.

  • Wu X  et al. Seipin Deficiency Accelerates Heart Failure Due to Calcium Handling Abnormalities and Endoplasmic Reticulum Stress in Mice. Front Cardiovasc Med 8:644128 (2021). PubMed: 33778025
  • Hanan M  et al. A Parkinson's disease CircRNAs Resource reveals a link between circSLC8A1 and oxidative stress. EMBO Mol Med 12:e11942 (2020). PubMed: 32715657
  • Gao H & Zhou Z Effect of Xylo-Oligosaccharides Supplementation by Drinking Water on the Bone Properties and Related Calcium Transporters in Growing Mice. Nutrients 12:N/A (2020). PubMed: 33228037
  • Sun F  et al. Detrimental Effect of C-Reactive Protein on the Cardiometabolic Cells and Its Rectifying by Metabolic Surgery in Obese Diabetic Patients. Diabetes Metab Syndr Obes 13:1349-1358 (2020). PubMed: 32425567
  • Yang HY  et al. The effect of Sirt1 deficiency on Ca2+ and Na+ regulation in mouse ventricular myocytes. J Cell Mol Med 24:6762-6772 (2020). PubMed: 32342656
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-6 of 6 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-NCX1 antibody [C2C12]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart)
Permeabilization
Yes - Tween-20
Specification
Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Sep 15 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NCX1 antibody [C2C12]

Excellent
Abreviews
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (coronary artery, heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer (pH 6)
Permeabilization
No
Specification
coronary artery, heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 27 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-NCX1 antibody [C2C12]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (hESC-derived cardiomyocyte)
Specification
hESC-derived cardiomyocyte
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Blocking step
Goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 04 2010

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NCX1 antibody [C2C12]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Normal Kidney)
Specification
Normal Kidney
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH6.0
Permeabilization
Yes - 0.05% Tween20
Blocking step
Normal Goat Serum in 1X PBS (0.05% Tween20) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 20 2010

Question

Our customer retested following your recommendation but the band still did not show in positive control (Hek293 cells).
She said that secondary antibody has been used for WB without any problem.
Please check attached file.

Best regards,

Read More

Abcam community

Verified customer

Asked on Jun 28 2012

Answer

Thank you and your customerfor taking the time totry the tipsand contact us. I am sorry to hear your customerhas had difficulty obtaining satisfactory results from this antibody.

The details your customerhas kindly provided will provide us with vital information for our monitoring of product quality.

I appreciate the time your customerhas spent in the laboratory and understandtheir concerns. It is regrettable the results have not been successful. Reviewing the details, I am sorry there are no further tips to provide on this occasion to help improve the results. I can suggest your customerhas regrettably received a bad vial.

I apologise for the inconvenience and am pleased to offer your customera free of charge replacement (also possible with an alternative antibody) or credit note in compensation.

In addition, I can suggest your customer may like to consider the following suggestions for future experiments:

Thank you and your customerfor your cooperation. I look forward to hearing from you with details of how your customerwould like to proceed.

Read More

Abcam Scientific Support

回复于 Jun 28 2012

Question

Product code: 2869
Lot number: GR37744-4

Inquiry: Our customer would like to know what size detected in human sample. Please let me know.

EA hy926 cell

Read More

Abcam community

Verified customer

Asked on Jun 22 2012

Answer

Thank you and your customer for your inquiry.

I can confirm that ab2869 detects a 120 kDa protein representing the sodium / calcium exchanger from guinea pig cardiac extract. The bands seen at 70 kDa and 160 kDa represent a proteolytic fragment and non-reduced exchanger respectively. The human protein shows the same molecular weight and I assume that bands at 70 kDa and 160 kDa also present a proteolytic fragment and anon-reduced exchanger respectively.

This publications shows the molecular weight of human NCX1:

Liu J et al. Functional sarcoplasmic reticulum for calcium handling of human embryonic stem cell-derived cardiomyocytes: insights for driven maturation. Stem Cells 25:3038-44 (2007).

This publication also uses milk for blocking and a over night incubation of ab2869.

I therefore recommend to following:

1. ) I suggest to use a positive control. Hek293 cells and human kidney are shown to be NCX1 positive.

2.) I can also suggest to check if the secondary antibody works.

3.) NCX1 is a multi pass protein and I therefore do recommend not to boil the samples but only to heat them up to 70C for 10 minutes.

I hope this information is helpful. please do not hesitate to contactme again with any further questions in this matter.

Read More

Abcam Scientific Support

回复于 Jun 22 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

通过邮件收到学术资源和促销信息 注册
研究类别(A-Z)
  • 肿瘤研究
  • 心血管研究
  • 细胞生物学
  • 发育生物学
  • 染色质及细胞核信号转导研究
  • 免疫学
  • 代谢
  • 微生物学
  • 神经生物学
  • 信号转导研究
  • 干细胞研究
产品种类(A-Z)
  • 一抗
  • 二抗
  • 生化试剂
  • 亚类对照
  • 流式细胞仪多色选择器
  • 试剂盒
  • 内参对照
  • 细胞裂解液
  • 多肽
  • 蛋白酶
  • 组织切片
  • 蛋白标签及细胞标记物
  • 相关试剂及工具
帮助与支持
  • 技术支持
  • 技术FAQ
  • 实验步骤与建议
  • 购买FAQ
  • RabMAb 产品
  • 培训课程
  • 按靶标浏览
企业
  • 企业资讯
  • 投资者关系
  • 公司新闻
  • 招贤纳士
  • 关于我们
活动通知
  • 商品展览
  • 会议
全球网站
  • abcam.com
  • abcam.co.jp
QR code for Weechat

微信扫码 关注我们

沪ICP备14046373号-1

沪公网安备 31011502007413号

  • 销售条款
  • 网站使用条款
  • Cookie政策
  • 隐私权政策
  • 法律
© 1998-2023 艾博抗(上海)贸易有限公司版权所有