Anti-NCR1抗体[n1D9] (ab14823)
Key features and details
- Mouse monoclonal [n1D9] to NCR1
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-NCR1抗体[n1D9]
参阅全部 NCR1 一抗 -
描述
小鼠单克隆抗体[n1D9] to NCR1 -
宿主
Mouse -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein, corresponding to amino acids 22-255 of Human NCR1
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阳性对照
- Flow Cyt: Human peripheral blood mononuclear cells or natural killer cells, PBMC cells, HeLa cells.
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常规说明
This product was changed from ascites to tissue culture supernatant on 24/01/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 10% Glycerol -
Concentration information loading...
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纯度
Tissue culture supernatant -
纯化说明
Purified from TCS. -
克隆
单克隆 -
克隆编号
n1D9 -
骨髓瘤
Sp2/0 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab14823于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. -
组织特异性
Selectively expressed by both resting and activated NK cells. -
序列相似性
Belongs to the natural cytotoxicity receptor (NCR) family.
Contains 2 Ig-like (immunoglobulin-like) domains. -
翻译后修饰
N-glycosylated.
O-glycosylated. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 9437 Human
- Omim: 604530 Human
- SwissProt: O76036 Human
- Unigene: 97084 Human
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别名
- Activating NK receptor NK p46 antibody
- CD335 antibody
- FLJ99094 antibody
see all
图片
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Flow cytometry analysis of NKp46 in HeLa cells. The cell was stained with ab14823 at 2-5µg for 1x106 cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black).
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Flow cytometry analysis of NKp46 in PBMC cells. The cell was stained with ab14823 at 2-5µg for 1x106 cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black).
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ab14823 staining human PBMC by flow cytometry.
This image was produced using the ascites version of this antibody.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab14823 被引用在 3 文献中.
- Qu S et al. Aneustat (OMN54) has aerobic glycolysis-inhibitory activity and also immunomodulatory activity as indicated by a first-generation PDX prostate cancer model. Int J Cancer 143:419-429 (2018). PubMed: 29441566
- Haberthur K et al. NKG2D ligand expression in pediatric brain tumors. Cancer Biol Ther 17:1253-1265 (2016). PubMed: 27834580
- Ascierto ML et al. Molecular signatures mostly associated with NK cells are predictive of relapse free survival in breast cancer patients. J Transl Med 11:145 (2013). IHC-P . PubMed: 23758773