Anti-NCOA62/SNW1抗体(ab70827)
Key features and details
- Rabbit polyclonal to NCOA62/SNW1
- Suitable for: IP, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-NCOA62/SNW1抗体
参阅全部 NCOA62/SNW1 一抗 -
描述
兔多克隆抗体to NCOA62/SNW1 -
宿主
Rabbit -
经测试应用
适用于: IP, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
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免疫原
Synthetic peptide corresponding to Human NCOA62/SNW1 aa 450-550.
Database link: Q13573 -
阳性对照
- HeLa, 293T and NIH3T3 whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70827于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
Use at 2-5 µg/mg of lysate.
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| WB |
1/2000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 59 kDa).
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| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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IP
Use at 2-5 µg/mg of lysate. |
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WB
1/2000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 59 kDa). |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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相关性
Nuclear receptor coactivator NCOA62/SNW1 (Nuclear Protein SkiP, SKIIP , ski-interacting protein) is a member of the SNW gene family, encodes a coactivator that enhances transcription from some Pol II promoters. This coactivator can bind to the ligand-binding domain of the vitamin D receptor and to retinoid receptors to enhance vitamin D-, retinoic acid-, estrogen-, and glucocorticoid-mediated gene expression. It can also interact with poly(A)-binding protein 2 to directly control the expression of muscle-specific genes at the transcriptional level. Finally, the protein may be involved in oncogenesis since it interacts with a region of SKI oncoproteins that is required for transforming activity. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 326578 Cow
- Entrez Gene: 22938 Human
- Entrez Gene: 66354 Mouse
- Omim: 603055 Human
- SwissProt: Q1JQE0 Cow
- SwissProt: Q13573 Human
- SwissProt: Q9CSN1 Mouse
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别名
- Bx 42 antibody
- Bx42 antibody
- Homolog of Drosophila BX42 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCOA62/SNW1 antibody (ab70827)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling NCOA62/SNW1 with ab70827 at 1/1000 (1µg/ml). Detection: DAB.
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Western blot - Anti-NCOA62/SNW1 antibody (ab70827)All lanes : Anti-NCOA62/SNW1 antibody (ab70827) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 59 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa, 40 kDa, 75 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation - Anti-NCOA62/SNW1 antibody (ab70827)Detection of Human NCOA62/SNW1 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70827 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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