Anti-NCKAP1L抗体(ab272631)
Key features and details
- Rabbit polyclonal to NCKAP1L
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-NCKAP1L抗体 -
描述
兔多克隆抗体to NCKAP1L -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human NCKAP1L aa 1-100.
Database link: P55160 -
阳性对照
- WB: RT4, U-251MG sp, Human plasma (IgG/HSA depleted), Human liver tissue and tonsil tissue. IHC-P: Human lymph tissue, small intestine tissue, liver tissue, lung tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272631于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
| 说明 |
|---|
|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
靶标
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组织特异性
Expressed only in cells of hematopoietic origin. -
序列相似性
Belongs to the HEM-1/HEM-2 family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3071 Human
- Omim: 141180 Human
- SwissProt: P55160 Human
- Unigene: 182014 Human
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别名
- 4930568P13Rik antibody
- AI463083 antibody
- HEM1 antibody
see all
图片
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Western blot - Anti-NCKAP1L antibody (ab272631)Lanes 2-6 : Anti-NCKAP1L antibody (ab272631) at 0.4 µg/ml
Lane 1 : Molecular weight ladder
Lane 2 : RT4
Lane 3 : U-251MG sp
Lane 4 : uman plasma (IgG/HSA depleted)
Lane 5 : Human liver tissue
Lane 6 : Human tonsil tissue -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCKAP1L antibody (ab272631)Paraffin-embedded formalin-fixed Human lymph tissue stained for NCKAP1L using ab272631 at 1/500 in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCKAP1L antibody (ab272631)Paraffin-embedded formalin-fixed Human small intestine tissue stained for NCKAP1L using ab272631 at 1/500 in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCKAP1L antibody (ab272631)Paraffin-embedded formalin-fixed Human liver tissue stained for NCKAP1L using ab272631 at 1/500 in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCKAP1L antibody (ab272631)Paraffin-embedded formalin-fixed Human lung tissue stained for NCKAP1L using ab272631 at 1/500 in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab272631 尚未被引用在任何文献中。