Anti-NCKAP1抗体- N-terminal (ab226302)
Key features and details
- Rabbit polyclonal to NCKAP1 - N-terminal
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-NCKAP1抗体- N-terminal
参阅全部 NCKAP1 一抗 -
描述
兔多克隆抗体to NCKAP1 - N-terminal -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide within Human NCKAP1 aa 1-50 (N terminal). The exact sequence is proprietary.
Database link: Q9Y2A7 -
阳性对照
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab226302 was affinity purified using an epitope specific to NCKAP1 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab226302于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 128 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 说明 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 128 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. -
组织特异性
Expressed in all tissues examined except peripheral blood leukocytes, with highest expression in brain, heart, and skeletal muscle. -
序列相似性
Belongs to the HEM-1/HEM-2 family. -
细胞定位
Cell membrane. Cell projection > lamellipodium membrane. At the interface between the lamellipodial actin meshwork and the membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 10787 Human
- Entrez Gene: 50884 Mouse
- Omim: 604891 Human
- SwissProt: Q9Y2A7 Human
- SwissProt: P28660 Mouse
- Unigene: 603732 Human
- Unigene: 25203 Mouse
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别名
- FLJ11291 antibody
- KIAA0587 antibody
- Membrane associated protein HEM2 antibody
see all
图片
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Western blot - Anti-NCKAP1 antibody - N-terminal (ab226302)All lanes : Anti-NCKAP1 antibody - N-terminal (ab226302) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 128 kDa
Exposure time: 10 secondsLysates prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-NCKAP1 antibody - N-terminal (ab226302)NCKAP1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226302 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226302 at 0.4 µg/ml.
Lane 1: ab226302 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab226302 尚未被引用在任何文献中。