Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-NCF1/p47-phox (phospho S304)抗体(ab63554)

Submit a review Q&A (3)References (4)
Western blot - Anti-NCF1/p47-phox (phospho S304) antibody (ab63554)

    Key features and details

    • Rabbit polyclonal to NCF1/p47-phox (phospho S304)
    • Suitable for: WB
    • Reacts with: Human, African green monkey
    • Isotype: IgG

    概述

    • 产品名称

      Anti-NCF1/p47-phox (phospho S304)抗体
      参阅全部 NCF1/p47-phox 一抗

    • 描述

      兔多克隆抗体to NCF1/p47-phox (phospho S304)

    • 宿主

      Rabbit

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Human, African green monkey

    • 免疫原

      Synthetic peptide corresponding to Human NCF1/p47-phox (phospho S304).
      Sequence:

      R-S-Sp-I-R

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 阳性对照

      • COS7 cells

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab63554于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
    说明
    WB
    1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

    靶标

    • 功能

      NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

    • 疾病相关

      Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.

    • 序列相似性

      Contains 1 PX (phox homology) domain.
      Contains 2 SH3 domains.

    • 细胞定位

      Cytoplasm.
    • Information by UniProt

    • 数据库链接

      • 别名

        • 47 kDa autosomal chronic granulomatous disease protein antibody
        • 47 kDa neutrophil oxidase factor antibody
        • NADPH oxidase organizer 2 antibody
        • NCF 47K antibody
        • NCF-1 antibody
        • NCF-47K antibody
        • Ncf1 antibody
        • NCF1_HUMAN antibody
        • Neutrophil cytosol factor 1 antibody
        • Neutrophil cytosolic factor 1 antibody
        • neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) antibody
        • Neutrophil NADPH oxidase factor 1 antibody
        • Nox organizer 2 antibody
        • Nox organizing protein 2 antibody
        • Nox-organizing protein 2 antibody
        • NOXO2 antibody
        • p47 phox antibody
        • p47-phox antibody
        • SH3 and PX domain containing protein 1A antibody
        • SH3 and PX domain-containing protein 1A antibody
        • SH3PXD1A antibody
        see all

      图片

      • Western blot - Anti-NCF1/p47-phox (phospho S304) antibody (ab63554)
        Western blot - Anti-NCF1/p47-phox (phospho S304) antibody (ab63554)
        All lanes : Anti-NCF1/p47-phox (phospho S304) antibody (ab63554) at 1/500 dilution

        Lane 1 : extracts from COS7 cells treated
        with UV (15mins)
        Lane 2 : A549 cells extracts
        Lane 3 : A549 cells extracts with immunizing peptide at 10 µg

        Lysates/proteins at 30 µg per lane.

        Predicted band size: 45 kDa
        Observed band size: 45 kDa

      文献 (4)

      发表研究结果有使用 ab63554？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab63554 被引用在 4 文献中.

      • Song P  et al. Geniposide protects against sepsis-induced myocardial dysfunction through AMPKa-dependent pathway. Free Radic Biol Med 152:186-196 (2020). PubMed: 32081748
      • Guo Y  et al. D-4F Ameliorates Contrast Media-Induced Oxidative Injuries in Endothelial Cells via the AMPK/PKC Pathway. Front Pharmacol 11:556074 (2020). PubMed: 33658920
      • Sowrirajan B  et al. Interleukin-27 Enhances the Potential of Reactive Oxygen Species Generation from Monocyte-derived Macrophages and Dendritic cells by Induction of p47(phox). Sci Rep 7:43441 (2017). PubMed: 28240310
      • Leverence JT  et al. Lipopolysaccharide-induced cytokine expression in alveolar epithelial cells: Role of PKC?-mediated p47phox phosphorylation. Chem Biol Interact : (2010). WB ; Human . PubMed: 20920494

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-3 of 3 Abreviews or Q&A

      Question

      Would like to use antibody in Flow Cytometry.

      Read More
      Answer

      DISCOUNT CODE: XXXXXXX
      Expiration date: 31-05-2013
      Value: £235

      I am very pleased to hear you would like to accept our offer and test ab63554 in Flow Cytometry. This code will give you ab63554 off your next order before the expiration date. To redeem this offer, please submit an Abreview for Flow Cytometry and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
      For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

      The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

      Read More

      Question

      Are there any antibodies available that detect the phosphorylated form of p47phox (a regulatory NADPH oxidase subunit) in mouse tissue? I have attached a recent paper which has published in antioxidants and redox signaling. Authors used an antibody from your company for the detection of phospho p47phox in cells extracted from humans.



      Thanks,

      Read More
      Answer

      Thank you for contacting us.

      Yes we have antibodies to detect the p47PHOX. The common alternative namesfor p47phox is NCF1, NOXO2 and SH3PXD1A.http://www.uniprot.org/uniprot/P14598

      The antibodies we have are; ab74095, ab63331, ab111855, ab115564, ab795, ab63554, ab107125. Please check the datasheets and let me know, if you want me to create a performa invoice for you.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Question

      No signal detected in blots of rat cell lysates; ab74095 gives expected signal with same samples

      Read More
      Answer

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number ***** for ab74095. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com