Anti-NCF1/p47-phox抗体(ab795)
Key features and details
- Goat polyclonal to NCF1/p47-phox
- Suitable for: WB
- Reacts with: Mouse, Human, Pig
- Isotype: IgG
概述
-
产品名称
Anti-NCF1/p47-phox抗体
参阅全部 NCF1/p47-phox 一抗 -
描述
山羊多克隆抗体to NCF1/p47-phox -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human, Pig
预测可用于: Rat, Rabbit, Cow -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: U937, Daudi, and U251 cell lysates, Mouse thymus and pig spleen tissue lysates.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Primary antibody说明
NCF1 is a cytosolic subunit of the multi-protein complex known as NADPH oxidase found in neutrophils. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
应用 | Ab评论 | 说明 |
---|---|---|
WB | (2) |
Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
1 hour primary incubation is recommended for this product. |
说明 |
---|
WB
Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). 1 hour primary incubation is recommended for this product. |
靶标
-
功能
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). -
疾病相关
Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections. -
序列相似性
Contains 1 PX (phox homology) domain.
Contains 2 SH3 domains. -
细胞定位
Cytoplasm. - Information by UniProt
-
数据库链接
- Entrez Gene: 653361 Human
- Entrez Gene: 17969 Mouse
- Entrez Gene: 114553 Rat
- Omim: 608512 Human
- SwissProt: P14598 Human
- SwissProt: Q09014 Mouse
- SwissProt: F1M707 Rat
- Unigene: 647047 Human
see all -
别名
- 47 kDa autosomal chronic granulomatous disease protein antibody
- 47 kDa neutrophil oxidase factor antibody
- NADPH oxidase organizer 2 antibody
see all
图片
-
Lane 1 : Anti-NCF1/p47-phox antibody (ab795) at 0.2 µg/ml
Lane 2 : Anti-NCF1/p47-phox antibody (ab795) at 0.01 µg/ml
Lanes 3-4 : Anti-NCF1/p47-phox antibody (ab795) at 0.3 µg/ml
Lane 1 : U937 (human histiocytic lymphoma cell line) cell lysate
Lane 2 : Daudi (human Burkitt's lymphoma cell line) cell lysate
Lane 3 : U-251 MG (formally U-373 MG) (human brain glioma cell line) whole cell lysate
Lane 4 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 45 kDaLysates prepared in RIPA buffer. Primary incubated for 1 hour. Detected by Western blot using chemiluminescence.
-
All lanes : Anti-NCF1/p47-phox antibody (ab795) at 1 µg/ml
Lane 1 : Mouse thymus lysate
Lane 2 : Pig spleen lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 45 kDaLysates prepared in RIPA buffer. Primary incubated for 1 hour. Detected by Western blot using chemiluminescence.
-
Anti-NCF1/p47-phox antibody (ab795) at 1.5 µg/ml +
Recombinant Human NCF1/p47-phox protein (ab98054) at 0.1 µg
Secondary
Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Exposure time: 8 minutes
数据表及文件
-
Datasheet download
文献 (20)
ab795 被引用在 20 文献中.
- Liu Y et al. Proteomic Analysis Revealed the Characteristics of Key Proteins Involved in the Regulation of Inflammatory Response, Leukocyte Transendothelial Migration, Phagocytosis, and Immune Process during Early Lung Blast Injury. Oxid Med Cell Longev 2021:8899274 (2021). PubMed: 34007409
- Lavier J et al. High-intensity exercise in hypoxia improves endothelial function via increased nitric oxide bioavailability in C57BL/6 mice. Acta Physiol (Oxf) 233:e13700 (2021). PubMed: 34089562
- Wang QS et al. NaHS or Lovastatin Attenuates Cyclosporine A-Induced Hypertension in Rats by Inhibiting Epithelial Sodium Channels. Front Pharmacol 12:665111 (2021). PubMed: 34122084
- Luo LP et al. Shenkang Injection and Its Three Anthraquinones Ameliorates Renal Fibrosis by Simultaneous Targeting IƙB/NF-ƙB and Keap1/Nrf2 Signaling Pathways. Front Pharmacol 12:800522 (2021). PubMed: 35002735
- Zhuang J et al. Age-related accumulation of advanced oxidation protein products promotes osteoclastogenesis through disruption of redox homeostasis. Cell Death Dis 12:1160 (2021). PubMed: 34907153