重组Anti-NCAM1抗体[EPR2566] (ab133345)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2566] to NCAM1
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-NCAM1抗体[EPR2566]
参阅全部 NCAM1 一抗 -
描述
兔单克隆抗体[EPR2566] to NCAM1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human NCAM1 aa 250-350 (extracellular). The exact sequence is proprietary.
Database link: P13591 -
阳性对照
- SH-SY5Y and Human fetal brain cell lysates; Human glioma tissue, Human cerebellum.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2566 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133345于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/5000. Detects a band of approximately 140-180 kDa (predicted molecular weight: 95 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/1000 - 1/5000. Detects a band of approximately 140-180 kDa (predicted molecular weight: 95 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. -
序列相似性
Contains 2 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 4684 Human
- Omim: 116930 Human
- SwissProt: P13591 Human
- Unigene: 503878 Human
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别名
- antigen MSK39 identified by monoclonal antibody 5.1H11 antibody
- antigen recognized by monoclonal antibody 5.1H11 antibody
- CD56 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EPR2566] (ab133345)
Immunohistochemical analysis of formalin-fixed paraffin-embedded human cerebellum labelling NCAM1 with ab214436 at a concentration of 0.5µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32min with ULTRA cell conditioning solution (CC1 pH8.5). ab214436 anti NCAM1 antibody was incubated at 37°C for 16min. Sections were counterstained is with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation (ab214436).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EPR2566] (ab133345)
Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human glioma tissue staining NCAM with ab133345 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-NCAM1 antibody [EPR2566] (ab133345) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : Human fetal brain cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-rabbit HRP at 1/2000 dilution
Predicted band size: 95 kDa
Observed band size: 140-180 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab133345 被引用在 10 文献中.
- Zhang HF et al. A MYCN-independent mechanism mediating secretome reprogramming and metastasis in MYCN-amplified neuroblastoma. Sci Adv 9:eadg6693 (2023). PubMed: 37611092
- Huo X et al. Methylation-based reclassification and risk stratification of skull-base chordomas. Front Oncol 12:960005 (2022). PubMed: 36439461
- Miyazaki Y et al. Adipose-derived mesenchymal stem cells differentiate into heterogeneous cancer-associated fibroblasts in a stroma-rich xenograft model. Sci Rep 11:4690 (2021). PubMed: 33633222
- Li X et al. Odontogenesis and neuronal differentiation characteristics of periodontal ligament stem cells from beagle dog. J Cell Mol Med 24:5146-5151 (2020). PubMed: 32202359
- Wiwatpanit T et al. Scaffold-Free Endometrial Organoids Respond to Excess Androgens Associated With Polycystic Ovarian Syndrome. J Clin Endocrinol Metab 105:N/A (2020). PubMed: 31614364
- Ongaratti BR et al. Gene and protein expression of E-cadherin and NCAM markers in non-functioning pituitary adenomas. Ann Diagn Pathol 38:59-61 (2019). PubMed: 30419428
- Yuan H et al. HPV positive neuroendocrine cervical cancer cells are dependent on Myc but not E6/E7 viral oncogenes. Sci Rep 7:45617 (2017). IHC-P, IF ; Human . PubMed: 28378747
- Mendes GA et al. Expression of E-cadherin, Slug and NCAM and its relationship to tumor invasiveness in patients with acromegaly. Braz J Med Biol Res 51:e6808 (2017). PubMed: 29267504
- Suarez ER et al. Chimeric antigen receptor T cells secreting anti-PD-L1 antibodies more effectively regress renal cell carcinoma in a humanized mouse model. Oncotarget 7:34341-55 (2016). PubMed: 27145284
- Lundgreen K et al. Lower muscle regenerative potential in full-thickness supraspinatus tears compared to partial-thickness tears. Acta Orthop 84:565-70 (2013). IHC-P ; Human . PubMed: 24171689