AB220360

重组Anti-NCAM1抗体[EPR21827]

Name: NCAM1抗体[EPR21827] (ab220360)| Abcam中文官网
Brand: Abcam Limited
SKU: AB220360
Price: 1743 CNY
Availability: InStock
Rating: 4 (2 reviews)

Anti-NCAM1 antibody [EPR21827]

20ul selling size
RabMAb
Recombinant
了解详情

(2 Reviews)

(32 Publications)

Anti-NCAM1 antibody [EPR21827] (ab220360) is a rabbit monoclonal antibody detecting NCAM1 in Western Blot, Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

查看别名

CD56, Ncam, Ncam1, Neural cell adhesion molecule 1, N-CAM-1, NCAM-1

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EPR21827] (AB220360)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EPR21827] (AB220360)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling NCAM1 with ab220360 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Positive staining on mouse cerebrum (PMID : 1705171). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rab it IgG H&L (HRP), Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Flow Cytometry - Anti-NCAM1 antibody [EPR21827] (AB220360)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-NCAM1 antibody [EPR21827] (AB220360)

Flow cytometric analysis of L-929 (mouse connective tissue fibroblast cell line) cell line (left panel) and Neuro-2a (mouse neuroblastoma cell line) cell line (right panel) labeling NCAM1 with ab220360 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

Gated on viable cells. Negative control : L-929.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EPR21827] (AB220360)

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling NCAM1 with ab220360 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Positive staining of ganglion (arrow) in mouse stomach (PMID : 1705171). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rab it IgG H&L (HRP), Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EPR21827] (AB220360)

Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling NCAM1 with ab220360 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Positive staining of ganglia (arrows) in rat colon (PMID : 1705171). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rab it IgG H&L (HRP), Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-NCAM1 antibody [EPR21827] (AB220360)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NCAM1 antibody [EPR21827] (AB220360)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (mouse neuroblastoma cell line) cells labeling NCAM1 with ab220360 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in Neuro-2a cell line.

Negative control : L-929 PMID : 9696812).

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

Supplier Data

Immunoprecipitation - Anti-NCAM1 antibody [EPR21827] (AB220360)

NCAM1 was immunoprecipitated from 0.35 mg of mouse brain lysate with ab220360 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220360 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : Mouse brain lysate 10 μg (Input).

Lane 2 : ab220360 IP in mouse brain lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab220360 in mouse brain lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

The 120,140 and 180kDa bands are different isoforms as reported in the literature (PMID : 26288071).

All lanes:

Immunoprecipitation - Anti-NCAM1 antibody [EPR21827] (ab220360)

Predicted band size: 94 kDa

Observed band size: 120-200 kDa

false

Supplier Data

Immunoprecipitation - Anti-NCAM1 antibody [EPR21827] (AB220360)

NCAM1 was immunoprecipitated from 0.35 mg of rat brain lysate with ab220360 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220360 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : Rat brain lysate 10 μg (Input).

Lane 2 : ab220360 IP in rat brain lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab220360 in rat brain lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

The 120,140 and 180kDa bands are different isoforms as reported in the literature (PMID : 26288071).

All lanes:

Immunoprecipitation - Anti-NCAM1 antibody [EPR21827] (ab220360)

Predicted band size: 94 kDa

Observed band size: 120-200 kDa

false

Supplier Data

Western blot - Anti-NCAM1 antibody [EPR21827] (AB220360)

Blocking/Dilution buffer : 5% NFDM/TBST.

Negative control : L-929 (PMID : 9696812)

All lanes:

Western blot - Anti-NCAM1 antibody [EPR21827] (ab220360) at 1/1000 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 10 µg

Lane 2:

L-929 (mouse connective tissue fibroblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 94 kDa

Observed band size: 140 kDa

true

Exposure time: 70s

Supplier Data

Western blot - Anti-NCAM1 antibody [EPR21827] (AB220360)

Exposure time : Lanes 1-2 : 26 seconds; Lane 3 : 70 seconds; Lane 4 : 3 minutes.

Blocking/Dilution buffer : 5% NFDM/TBST.

The 120,140 and 180kDa bands are different isoforms as reported in the literature (PMID : 26288071).

All lanes:

Western blot - Anti-NCAM1 antibody [EPR21827] (ab220360) at 1/5000 dilution

Lane 1:

Mouse brain lysate at 20 µg

Lane 2:

Mouse cerebellum lysate at 20 µg

Lane 3:

Rat brain lysate at 20 µg

Lane 4:

Rat cerebellum lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 94 kDa

Observed band size: 120 kDa,140 kDa,180 kDa

true

不同偶联物与剂型 (7)

578 PE

PE Anti-NCAM1 antibody [EPR21827]
660 APC

APC Anti-NCAM1 antibody [EPR21827]
Carrier free

Anti-NCAM1 antibody [EPR21827] - BSA and Azide free
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-NCAM1 antibody [EPR21827]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-NCAM1 antibody [EPR21827]
519 FITC

FITC Anti-NCAM1 antibody [EPR21827]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-NCAM1 antibody [EPR21827]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR21827

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat

应用

WB, IHC-P, IP, Flow Cyt, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/600", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "" } } }

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产品详情

What is this antibody validated in?
Anti-NCAM1 antibody [EPR21827] (ab220360) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Mouse, Rat samples.

What is the molecular weight of NCAM1?
Anti-NCAM1 [EPR21827] (ab220360) specifically detects a band for NCAM1 (UniProt: P13595) at a molecular weight of 95kDa.

Trusted by the scientific community
Anti-NCAM1 [EPR21827] (ab220360) was first used in a scientific publication in 2018 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR21827] also available for your convenience: ab220360, Carrier free - ab231826, Alexa Fluor® 488 - ab237380, PE - ab237381, FITC - ab237382, APC - ab237383, Alexa Fluor® 647 - ab302694, Alexa Fluor® 594 - ab303720

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

NCAM1 also known as Neural Cell Adhesion Molecule 1 or CD56 is an important cell surface glycoprotein that plays a role in cell-cell adhesion. It has a molecular mass of approximately 120-140 kDa. NCAM1 is widely expressed in various tissues including the nervous system skeletal muscle and certain hematopoietic cells. In flow cytometry and immunohistochemistry NCAM1's expression is extensively studied to track and analyze cell populations using techniques like NCAM1 ELISA for quantification.

Biological function summary

NCAM1 influences neuronal growth survival and synaptic plasticity. This protein is part of the immunoglobulin superfamily and contributes to dynamic interactions during development. NCAM1 exists on cell membranes assisting in the formation and maintenance of tissue structures. In particular cell adhesion molecule CAL53 interacts with NCAM1 highlighting its involvement in synaptic connections. Additionally NCAM1 impacts cellular migration and differentiation particularly in myogenic processes involving MY31's pathway.

Pathways

The NCAM1 protein participates in the MAPK signaling pathway and the PI3K/AKT pathway both essential in cellular growth and survival. NCAM1 acts alongside ERIC1 aiding in the transduction of signals that regulate cytoskeletal rearrangement. These pathways influence cellular communication and responses to external stimuli further enhancing tissue development and regeneration mechanisms.

NCAM1 is associated with neurodegenerative conditions like Alzheimer's disease and certain types of cancer including neuroblastoma. Changes in NCAM1 expression or function can disrupt normal cell adhesion and signaling leading to pathological states. The protein's interaction with cell surface markers such as CD56 mouse marker helps in the diagnosis and therapy of these disorders providing potential therapeutic targets or biomarkers for clinical applications.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.

See full target information Ncam1

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文献 (32)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 30:456 PubMed40762017

2025

Acute myeloid leukemia with plasmacytoid dendritic cell proliferation: A case report and literature review.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixin Pei,Yi Zhang,Han Xu,Pei Pei,Zhengyang Zhang,Hongxia Wang,Bei Zhang,Junjun Bai,Yingxin Zhao,Jingjing Gu,Zhiyu Fang,Miaomiao Liu,Qinglin Song

Oncology letters 29:225 PubMed40110578

2025

Adrenal cortical carcinoma with non‑islet cell tumor hypoglycemia: A case report.

Applications

Unspecified application

Species

Unspecified reactive species

Yunpeng Li,Rui Yang,Liang Zhang,Jian Wang,Faying Yang,Song Tu,Jun Qian,Jiaxi Yao

Frontiers in oncology 15:1480461 PubMed39926286

2025

Mature tertiary lymphoid structure associated CD103+ CD8+ Trm cells determined improved anti-tumor immune in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Fang,Shuru Chen,Xiaoyue Chen,Wei Zou,Di Chen,Yukang Huang,Chucheng Wu

Histology and histopathology 40:1061-1071 PubMed39410869

2024

DNMT1 silencing induces KIR2DL1/2/3 expression via methylation to alleviate graft--host disease after allogeneic hematopoietic stem cell transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Zhang,Shuling Yu,Miao Zhou,Xiao Yan,Huiling Zhu,Lixia Sheng,Yi Zhang,Shujun Yang,Guifang Ouyang

NPJ breast cancer 10:80 PubMed39277578

2024

Mcam stabilizes a luminal progenitor-like breast cancer cell state via Ck2 control and Src/Akt/Stat3 attenuation.

Applications

Unspecified application

Species

Unspecified reactive species

Ozlen Balcioglu,Brooke L Gates,David W Freeman,Berhane M Hagos,Elnaz Mirzaei Mehrabad,David Ayala-Talavera,Benjamin T Spike

Oncology letters 28:468 PubMed39119236

2024

Myeloid/lymphoid neoplasms with eosinophilia and FGFR1 rearrangement t(8;13)(p11;q12): A case report and literature review.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Jie Guo,Meng-Xue Ma,Tian Tian,Jing-Nan Zhang,Xiao-Nan Guo,Shukai Qiao

Journal of nanobiotechnology 22:244 PubMed38735969

2024

Electrical aligned polyurethane nerve guidance conduit modulates macrophage polarization and facilitates immunoregulatory peripheral nerve regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Yiting Sun,Yinglong Zhang,Yibo Guo,Dongming He,Wanlin Xu,Wei Fang,Chenping Zhang,Yi Zuo,Zhen Zhang

Annals of medicine and surgery (2012) 86:2644-2650 PubMed38694326

2024

S100A6 could not promote the differentiation of Calu-6 lung cancer cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Li,Ting Wang,Dan Dang

Journal of the American Society of Nephrology : JASN 35:854-869 PubMed38652545

2024

Spatiotemporal Landscape of Kidney Tubular Responses to Glomerular Proteinuria.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Faivre,Milica Bugarski,Anna Rinaldi,Imene B Sakhi,Thomas Verissimo,David Legouis,Joseph M Rutkowski,Sara Correia,Monika Kaminska,Delal Dalga,Daniele Malpetti,Pietro E Cippa,Sophie de Seigneux,Andrew M Hall

Communications biology 7:63 PubMed38191598

2024

Single-cell transcriptomic analyses of tumor microenvironment and molecular reprograming landscape of metastatic laryngeal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Sun,Sheng Chen,Yongping Lu,Zhenming Xu,Weineng Fu,Wei Yan

View all publications

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For licensing inquiries, please contact partnerships@abcam.com

重组Anti-NCAM1抗体[EPR21827]

Anti-NCAM1 antibody [EPR21827]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EPR21827] (AB220360)

Flow Cytometry - Anti-NCAM1 antibody [EPR21827] (AB220360)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EPR21827] (AB220360)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EPR21827] (AB220360)

Immunocytochemistry/ Immunofluorescence - Anti-NCAM1 antibody [EPR21827] (AB220360)

Immunoprecipitation - Anti-NCAM1 antibody [EPR21827] (AB220360)

Immunoprecipitation - Anti-NCAM1 antibody [EPR21827] (AB220360)

Western blot - Anti-NCAM1 antibody [EPR21827] (AB220360)

Western blot - Anti-NCAM1 antibody [EPR21827] (AB220360)