Anti-NBR1抗体- C-terminal (ab226360)
Key features and details
- Rabbit polyclonal to NBR1 - C-terminal
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-NBR1抗体- C-terminal
参阅全部 NBR1 一抗 -
描述
兔多克隆抗体to NBR1 - C-terminal -
宿主
Rabbit -
经测试应用
适用于: IP, WBmore details -
种属反应性
与反应: Human
预测可用于: Orangutan
-
免疫原
Synthetic peptide within Human NBR1 aa 916-966 (C terminal). The exact sequence is proprietary.
Database link: Q14596 -
阳性对照
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab226360 was affinity purified using an epitope specific to NBR1 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab226360于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
Use at 2-10 µg/mg of lysate.
|
|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 107 kDa.
|
| 说明 |
|---|
|
IP
Use at 2-10 µg/mg of lysate. |
|
WB
1/2000 - 1/10000. Predicted molecular weight: 107 kDa. |
靶标
-
功能
Acts probably as a receptor for selective autophagosomal degradation of ubiquitinated targets. -
序列相似性
Contains 1 OPR domain.
Contains 1 UBA domain.
Contains 1 ZZ-type zinc finger. -
结构域
The OPR domain mediates interaction with SQSTM1. -
细胞定位
Cytoplasm. Cytoplasmic vesicle > autophagosome. Lysosome. Cytoplasm > myofibril > sarcomere > M line. In cardiac muscles localizes to the sarcomeric M line (By similarity). Is targeted to lysosomes for degradation. - Information by UniProt
-
数据库链接
- Entrez Gene: 4077 Human
- Entrez Gene: 100174370 Orangutan
- Omim: 166945 Human
- SwissProt: Q14596 Human
- SwissProt: Q5RC94 Orangutan
- Unigene: 277721 Human
- Unigene: 708158 Human
-
别名
- 1A1 3B antibody
- 1A13B antibody
- B box protein antibody
see all
图片
-
Western blot - Anti-NBR1 antibody - C-terminal (ab226360)All lanes : Anti-NBR1 antibody - C-terminal (ab226360) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 107 kDa
Exposure time: 30 secondsCell lysates were prepared using NETN lysis buffer.
-
Immunoprecipitation - Anti-NBR1 antibody - C-terminal (ab226360)NBR1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226360 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226360 at 1 µg/ml.
Lane 1: ab226360 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab226360 尚未被引用在任何文献中。