AB250919

重组Anti-NBEAL2抗体[EPR14501(B)] - BSA and Azide free

Anti-NBEAL2 antibody [EPR14501(B)] - BSA and Azide free

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal NBEAL2 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 2 publications.

查看别名

KIAA0540, UNQ253/PRO290, NBEAL2, Neurobeachin-like protein 2

1 Images

Supplier Data

Western blot - Anti-NBEAL2 antibody [EPR14501(B)] - BSA and Azide free (AB250919)

This data was developed using ab187162, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NBEAL2 antibody [EPR14501(B)] - N-terminal (<a href='/products/primary-antibodies/nbeal2-antibody-epr14501b-n-terminal-ab187162'>ab187162</a>) at 1/1000 dilution

All lanes:

Human platelet lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 303 kDa

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不同偶联物与剂型 (1)

Unconjugated

Anti-NBEAL2 antibody [EPR14501(B)] - N-terminal

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR14501(B)

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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产品详情

ab250919 is the carrier-free version of ab187162.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The NBEAL2 protein also known as Neurobeachin-like 2 functions as a large protein with a mass of approximately 304 kDa. It has an important role in vesicle-mediated transport within cells. NBEAL2 is expressed mostly in hematopoietic tissues including bone marrow and peripheral blood. The protein structure includes a BEACH domain WD repeats and a leucine zipper motif which contribute to its involvement in cellular trafficking processes.

Biological function summary

The NBEAL2 protein plays an important role in platelet formation and maintenance. It appears necessary for the development of α-granules in platelets which are essential for proper blood clotting. These α-granules store numerous proteins such as clotting factors that are important in hemostasis. While NBEAL2 does not form its own complex it interacts with various cellular machinery involved in vesicle trafficking.

Pathways

NBEAL2 serves as a critical component in pathways regulating platelet production and release. Its functions intersect with the megakaryocyte development pathway an essential pathway for platelet biogenesis. NBEAL2 shows close interaction with proteins like WASP which also contribute to thrombopoiesis. Its activity ensures the proper formation and function of platelet precursor cells.

Several findings connect the NBEAL2 protein to gray platelet syndrome (GPS). GPS is a rare bleeding disorder characterized by the deficiency or absence of α-granules in platelets. Mutations in NBEAL2 are frequently linked to the development of this syndrome. Moreover the protein's role in platelet function also ties it to potential contributions in disorders related to thrombosis. Understanding NBEAL2's interactions especially with other proteins involved in platelet formation like MYH9 offers further insights into its implications in hematological conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Probably involved in thrombopoiesis. Plays a role in the development or secretion of alpha-granules, that contain several growth factors important for platelet biogenesis.

See full target information NBEAL2

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of radiation research 64:833-841 PubMed37439405

2023

Expression of TMEM59L associated with radiosensitive in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dezhi Gao,Peng Wang,Lin Zhi,Shibin Sun,Xiaoguang Qiu,Yanwei Liu

Nature communications 14:3728 PubMed37349339

2023

NBEAL2 deficiency in humans leads to low CTLA-4 expression in activated conventional T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Laure Delage,Francesco Carbone,Quentin Riller,Jean-Luc Zachayus,Erwan Kerbellec,Armelle Buzy,Marie-Claude Stolzenberg,Marine Luka,Camille de Cevins,Georges Kalouche,Rémi Favier,Alizée Michel,Sonia Meynier,Aurélien Corneau,Caroline Evrard,Nathalie Neveux,Sébastien Roudières,Brieuc P Pérot,Mathieu Fusaro,Christelle Lenoir,Olivier Pellé,Mélanie Parisot,Marc Bras,Sébastien Héritier,Guy Leverger,Anne-Sophie Korganow,Capucine Picard,Sylvain Latour,Bénédicte Collet,Alain Fischer,Bénédicte Neven,Aude Magérus,Mickaël Ménager,Benoit Pasquier,Frédéric Rieux-Laucat

View all publications

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重组Anti-NBEAL2抗体[EPR14501(B)] - BSA and Azide free