Anti-NBAS抗体(ab122370)
Key features and details
- Rabbit polyclonal to NBAS
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-NBAS抗体
参阅全部 NBAS 一抗 -
描述
兔多克隆抗体to NBAS -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
antigen sequence: AKGDLKFPLK IFQHSKPDLQ QKIIPDQDQL MAIALECIYT CERNDQLCLC YDLLECLPER GYGDKTEATT KLHDMVDQLE QILSVS, corresponding to amino acids 962-1047 of Human NBAS (UniProt ID: A2RRP1).
-
阳性对照
- Human heart muscle tissue
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122370于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
| 说明 |
|---|
|
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
靶标
-
组织特异性
Broadly expressed, with highest levels in heart and skeletal muscle, and lowest levels in liver, small intestine and thymus. Up-regulated together with N-myc in some neuroblastoma cell lines. -
序列相似性
Contains 2 WD repeats. - Information by UniProt
-
数据库链接
- Entrez Gene: 51594 Human
- Omim: 608025 Human
- SwissProt: A2RRP1 Human
- Unigene: 467759 Human
-
别名
- NBAS antibody
- NBAS_HUMAN antibody
- Neuroblastoma-amplified gene protein antibody
- Neuroblastoma-amplified sequence antibody
图片
-
Immunocytochemistry/ Immunofluorescence - Anti-NBAS antibody (ab122370)Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm and golgi apparatus. Recommended concentration of ab122370 1-4 µg/ml. Cells treated with PFA/Triton X-100.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NBAS antibody (ab122370)ab122370, at a 1/50 dilution, staining NBAS in paraffin embedded Human heart muscle tissue by Immunohistochemistry.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab122370 被引用在 2 文献中.
- Launay N et al. RINT1 deficiency disrupts lipid metabolism and underlies a complex hereditary spastic paraplegia. J Clin Invest 133:N/A (2023). PubMed: 37463447
- Xu D et al. Rab18 promotes lipid droplet (LD) growth by tethering the ER to LDs through SNARE and NRZ interactions. J Cell Biol 217:975-995 (2018). PubMed: 29367353