Anti-Natriuretic多肽A抗体[M622709] (ab2093)

Reviews (2)Q&A (5)References (2)

Key features and details

  • Mouse monoclonal [M622709] to Natriuretic peptides A
  • Suitable for: IHC-P, Flow Cyt, RIA
  • Reacts with: Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-Natriuretic多肽A抗体[M622709]
    参阅全部 Natriuretic peptides A 一抗

  • 描述

    小鼠单克隆抗体[M622709] to Natriuretic多肽A

  • 宿主

    Mouse

  • 特异性

    Affinity Constant: 2.2 x 1010 L/M

  • 经测试应用

    适用于: IHC-P, Flow Cyt, RIAmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide corresponding to Human Natriuretic peptides A conjugated to bovine serum albumin.
    Database link: P01160

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2093于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P (2)
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration. PubMed: 20080656

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
RIA
Use at an assay dependent concentration.
说明
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration. PubMed: 20080656

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
RIA
Use at an assay dependent concentration.

靶标

  • 功能

    Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.

  • 疾病相关

    Atrial standstill 2
    Atrial fibrillation, familial, 6

  • 序列相似性

    Belongs to the natriuretic peptide family.

  • 翻译后修饰

    Cleaved by CORIN upon secretion to produce the functional hormone.
    Atrial natriuretic factor: Cleaved by MME. The cleavage initiates degradation of the factor and thereby regulate its activity.

  • 细胞定位

    Secreted.
  • Information by UniProt

  • 数据库链接

    • 别名

      • ANF antibody
      • ANF_HUMAN antibody
      • ANP antibody
      • ATFB6 antibody
      • Atrial natriuretic factor antibody
      • Atrial natriuretic peptide antibody
      • Atriopeptin antibody
      • Cardiodilatin antibody
      • Cardiodilatin related peptide antibody
      • Cardiodilatin-related peptide antibody
      • Cardionatrin antibody
      • CDD ANF antibody
      • CDD antibody
      • CDD-ANF antibody
      • CDP antibody
      • Natriuretic peptide A antibody
      • Natriuretic peptide precursor A antibody
      • NPPA antibody
      • PND antibody
      • Prepronatriodilatin antibody
      see all

    文献 (2)

    发表研究结果有使用 ab2093？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab2093 被引用在 2 文献中.

    • Ahmed A  et al. Natriuretic peptide system expression in murine and human submandibular salivary glands: a study of the spatial localisation of ANB, BNP, CNP and their receptors. J Mol Histol 51:3-13 (2020). PubMed: 31722080
    • Therrien JP  et al. A gene therapy approach for long-term normalization of blood pressure in hypertensive mice by ANP-secreting human skin grafts. Proc Natl Acad Sci U S A 107:1178-83 (2010). IHC-P, Flow Cyt ; Human . PubMed: 20080656

    客户评价及客户问答

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    1-7 of 7 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Natriuretic peptides A antibody [M622709]

     Excellent
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Salivary gland)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    Salivary gland
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2000µg/mL · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Isabelle Miletich

    Verified customer

    提交于 Jan 05 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Natriuretic peptides A antibody [M622709]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Heart tissue including atrium)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    Heart tissue including atrium
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2000µg/mL · Temperature: 21D°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Isabelle Miletich

    Verified customer

    提交于 Nov 04 2016

    Question

    Customer has requested MSDS and CoA for their order #1156531. There are 2 items on the order, and they are as follows: Anti-ANP antibody [M622709] (ab2093) Anti-Urodilatin antibody (ab14319) Customer info is on the order. Thanks :)

    Read More
    Answer

    Thank you for contacting us.


    I have attached the CoCs for ab14319, Anti-Urodilatin antibody and for ab2093 Anti-ANP antibody [M622709] as well the SDS for ab2093. I have also asked our documents team to create the SDS for ab14319. This will be added to the webpage for this product very early next week and can be downloaded directly from there. The page for this product can be found at this link:

    https://www.abcam.com/urodilatin-antibody-ab14319.html


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.



    Use our products? Submit an Abreview. Earn rewards!

    https://www.abcam.com/abreviews

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    Question

    I would like to know if the purified form of the whole ANP protein that has been used to raise this antibody is available commercially. Thanks,

    Read More
    Answer

    Thank you for your enquiry. Unfortunately, we do not have the protein that was used to make this antibody in our catalogue and I am afraid that I do not know if it is commercially available. I am sorry I can't be of more assistance in this occasion. Should you require anything further, please do not hesitate to contact me.

    Read More

    Question

    What's the antigen sequence for this antibody? What part of the protein was the antibody made to?

    Read More
    Answer

    Thank you for your enquiry. ab2093 was generated using Atrial Natriuretic Peptide whole molecule as the immunogen. The peptide sequence has accession number: CAA25700 and the following sequence: MSSFSTTTVS FLLLLAFQLL GQTRANPMYN AVSNADLMDF KNLLDHLEEK MPLEDEVVPP QVLSEPNEEA GAALSPLPEV PPWTGEVSPA QRDGGALGRG PWDSSDRSAL LKSKLRALLT APRSLRRSSC FGGRMDRIGA QSGLGCNSFR YRR I hope this information helps, please do not hesitate to contact me should you require further assistance.

    Read More

    Question

    Customer would like to know - what is the molecular weight of this antibody?

    Read More
    Answer

    Clone M622709 is subtype IgG1. The approximate molecular weight of IgG1 is 148,000 daltons. This antibody has only been tested for application in Immunohistochemistry (Formalin-fixed paraffin-embedded sections)and Radioimmunoassay.

    Read More

    Question

    I am using this antibody in WB on human left ventricle whole cell extract. I am getting a band at 40kDa, where the expected molecular weight should be approximately 17kDa. I'm wondering if it is detecting a precursor form of ANP? Any help you could give me would be very much appreciated.

    Read More
    Answer

    Thank you for your enquiry and your interest in our products. As my colleagues possibly explained to you over the telephone, this antibody has not been tested for Western blot application. According to the datasheet, ab2093 recognizes the native form of the human protein (it is suitable for IHC and RIA) but so far we do not have any data regarding its suitability for WB. It may well be that the antibody recognizes the precursor form of ANP. It is also possible that the protein is undergone some post-translational modification, however we do not have any information about it. If you need anything further or any help then please let us know.

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