重组Anti-Natriuretic多肽A抗体[EPR20247] - BSA and Azide free (ab225873)
![Multiplex immunohistochemistry - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)](https://www.abcam.cn/ps/products/225/ab225873/Images/ab225873-517771-anti-titin-antibody-epr26167-75-multiplex-immunohistochemistry-human.jpg "Multiplex immunohistochemistry - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20247] to Natriuretic peptides A - BSA and Azide free
- Suitable for: WB, IHC-P, IHC-Fr, IP
- Reacts with: Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Natriuretic多肽A抗体[EPR20247] - BSA and Azide free
参阅全部 Natriuretic peptides A 一抗 -
描述
兔单克隆抗体[EPR20247] to Natriuretic多肽A - BSA and Azide free -
宿主
Rabbit -
特异性
This antibody is not suitable to detect ANP in H9c2 cell line.
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经测试应用
适用于: WB, IHC-P, IHC-Fr, IPmore details -
种属反应性
与反应: Rat, Human -
免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human fetal heart and rat heart tissue lysates. IHC-P: Human heart, rat auricle and rat ventricle tissues. IHC-Fr: Rat heart tissue. IP: Rat heart lysate. mIHC: Human cardiac muscle tissue.
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常规说明
ab225873 is the carrier-free version of ab209232.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR20247 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab225873于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 16 kDa.
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| IHC-Fr |
Use at an assay dependent concentration.
Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). |
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| IP |
Use at an assay dependent concentration.
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| 说明 |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 16 kDa. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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IHC-Fr
Use at an assay dependent concentration. Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). |
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IP
Use at an assay dependent concentration. |
靶标
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功能
Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. -
疾病相关
Atrial standstill 2
Atrial fibrillation, familial, 6 -
序列相似性
Belongs to the natriuretic peptide family. -
翻译后修饰
Cleaved by CORIN upon secretion to produce the functional hormone.
Atrial natriuretic factor: Cleaved by MME. The cleavage initiates degradation of the factor and thereby regulate its activity. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 4878 Human
- Entrez Gene: 24602 Rat
- Omim: 108780 Human
- SwissProt: P01160 Human
- SwissProt: P01161 Rat
- Unigene: 75640 Human
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别名
- ANF antibody
- ANF_HUMAN antibody
- ANP antibody
see all
图片
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Multiplex immunohistochemistry - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)
Fluorescence multiplex immunohistochemical analysis of the human cardiac muscle (Formalin/PFA-fixed paraffin-embedded sections).
Panel A: merged staining of anti-delta Sarcoglycan (ab137101, red; Opal™690), anti-Titin (ab307446, green; Opal™520) and anti-Natriuretic peptides A (ab209232, magenta; Opal™570) on human cardiac muscle. Panel B: anti-Titin displayed nucleus and cytoplasm expression. Panel C: anti-Natriuretic peptides A displayed granular cytoplasmic expression. Panel D: anti-delta Sarcoglycan displayed membrane expression. Opal Polymer HRP Ms + Rb was used as a secondary antibody.
The section was incubated in three rounds of staining: in the order of ab137101 at 1/1000 (1.043 μg/ml) dilution, ab307446 at 1/500 (0.95 μg/ml) dilution, and ab209232 at 1/3000 (0.241 μg/ml) dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. DAPI (blue) was used as a nuclear counter stain.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209232).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)](https://www.abcam.cn/ps/products/225/ab225873/Images/ab225873-324891-anti-natriuretic-peptides-a-antibody-epr20247-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)Immunohistochemical analysis of paraffin-embedded rat auricle tissue labeling Natriuretic peptides A with ab209232 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Granularly cytoplasmic and perinuclear staining on rat auricle (PMID: 2942710, PMID: 1824903).
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209232).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)](https://www.abcam.cn/ps/products/225/ab225873/Images/ab225873-324890-anti-natriuretic-peptides-a-antibody-epr20247-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)Immunohistochemical analysis of paraffin-embedded rat ventricle tissue labeling Natriuretic peptides A with ab209232 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Weak staining on rat ventricle (PMID: 2942710, PMID: 1824903).
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209232).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Frozen sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)](https://www.abcam.cn/ps/products/225/ab225873/Images/ab225873-3-ab209232279617209232IHCFr.jpg)
Immunohistochemistry (Frozen sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat heart tissue labeling Natriuretic peptides A with ab209232 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Cytoplasmic staining on the auricula of rat heart (PMID: 2942710, PMID: 1824903).
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209232).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)](https://www.abcam.cn/ps/products/225/ab225873/Images/ab225873-2-ab209232279616209232IP.jpg)
Immunoprecipitation - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)Natriuretic peptides A was immunoprecipitated from 0.35 mg of rat heart lysate with ab209232 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab209232 at 1/500 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Rat heart lysate 10 µg (Input).
Lane 2: ab209232 IP in rat heart lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209232 in rat heart lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209232).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)](https://www.abcam.cn/ps/products/225/ab225873/Images/ab225873-286691-225873IHCa.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)Immunohistochemical analysis of paraffin-embedded human heart tissue labeling Natriuretic peptides A with ab209232 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Granularly cytoplasmic and perinuclear staining on human heart (PMID: 2942710, PMID: 1824903).
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209232).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)](https://www.abcam.cn/ps/products/225/ab225873/Images/ab225873-541084-anti-natriuretic-a-antibody-epr20247-western-blotting-rat.jpg)
Western blot - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)All lanes : Anti-Natriuretic peptides A antibody [EPR20247] (ab209232) at 1/1000 dilution
Lane 1 : Untreated H9c2 (2-1) (rat cardiac myoblast cell) whole cell lysate
Lane 2 : H9c2 (2-1) (rat cardiac myoblast cell) treated with LPS (2ug/ml, 16h) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 16 kDa
Exposure time: 180 secondsThis data was developed using ab209232, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer concentration: 5% NFDM/TBST.
ab181602 GAPDH is used a loading control at 1/100000 dilution.
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![Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)](https://www.abcam.cn/ps/products/225/ab225873/Images/ab225873-6-benefits-of-recombinant-antibodies.png)
Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab225873 尚未被引用在任何文献中。
