重组Anti-NASP抗体[EPR12827] (ab181169)
![Western blot - Anti-NASP antibody [EPR12827] (ab181169)](https://www.abcam.cn/ps/products/181/ab181169/Images/ab181169-214728-anti-nasp-antibody-epr12827-western-blot.jpg "Western blot - Anti-NASP antibody [EPR12827] (ab181169)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12827] to NASP
- Suitable for: WB, ICC/IF, IP, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-NASP抗体[EPR12827]
参阅全部 NASP 一抗 -
描述
兔单克隆抗体[EPR12827] to NASP -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IP, Flow Cyt (Intra)more details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa, Caco2, 293 or NTERA2/D1 lysate, 293 or HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR12827 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab181169于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 85 kDa.
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| ICC/IF |
1/100 - 1/250.
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| IP |
1/40 - 1/60.
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| Flow Cyt (Intra) |
1/40.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 85 kDa. |
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ICC/IF
1/100 - 1/250. |
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IP
1/40 - 1/60. |
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Flow Cyt (Intra)
1/40. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA. -
组织特异性
Isoform 1 is testis- and sperm-specific. -
序列相似性
Belongs to the NASP family.
Contains 3 TPR repeats. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 4678 Human
- Entrez Gene: 50927 Mouse
- Entrez Gene: 298441 Rat
- Omim: 603185 Human
- SwissProt: P49321 Human
- SwissProt: Q99MD9 Mouse
- SwissProt: Q66HD3 Rat
- Unigene: 319334 Human
see all -
别名
- 5033430J04Rik antibody
- AI131596 antibody
- AI317140 antibody
see all
图片
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Western blot - Anti-NASP antibody [EPR12827] (ab181169)All lanes : Anti-NASP antibody [EPR12827] (ab181169) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Caco-2 cell lysate
Lane 3 : 293 cell lysate
Lane 4 : NTERA2/D1 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 85 kDa160KDa: tNASP
62KDa: sNASP -
![Immunoprecipitation - Anti-NASP antibody [EPR12827] (ab181169)](https://www.abcam.cn/ps/products/181/ab181169/Images/ab181169-214736-anti-nasp-antibody-epr12827-immunoprecipitation.jpg)
Immunoprecipitation - Anti-NASP antibody [EPR12827] (ab181169)Detection of Human NASP by Western Blot of Immunoprecipitate. Caco2 whole cell lysate loaded, ab181169 immunoprecipitated NASP at a 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary used at a 1/1000 dilution.
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![Flow Cytometry (Intracellular) - Anti-NASP antibody [EPR12827] (ab181169)](https://www.abcam.cn/ps/products/181/ab181169/Images/ab181169-214735-anti-nasp-antibody-epr12827-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-NASP antibody [EPR12827] (ab181169)Intracellular Flow Cytometry analysis of HeLa cells using ab181169at a 1/40 dilution (red) or aRabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary usedat a 1/40 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-NASP antibody [EPR12827] (ab181169)](https://www.abcam.cn/ps/products/181/ab181169/Images/ab181169-214733-anti-nasp-antibody-epr12827-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NASP antibody [EPR12827] (ab181169)Immunofluorescence analysis of 293 cells (fixative 4% paraformaldehyde) labeling NASP with ab181169 at a 1/250 dilution.Goat anti rabbit IgG (Alexa Fluor® 555) secondary used at a 1/200 diution.
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![Anti-NASP antibody [EPR12827] (ab181169)](https://www.abcam.cn/ps/products/181/ab181169/Images/ab181169-5-benefits-of-recombinant-antibodies.png)
Anti-NASP antibody [EPR12827] (ab181169)
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (6)
ab181169 被引用在 6 文献中.
- Carraro M et al. DAXX adds a de novo H3.3K9me3 deposition pathway to the histone chaperone network. Mol Cell 83:1075-1092.e9 (2023). PubMed: 36868228
- Li J et al. SRSF10 regulates proliferation of neural progenitor cells and affects neurogenesis in developing mouse neocortex. iScience 26:107042 (2023). PubMed: 37360696
- Sereesongsaeng N et al. Cathepsin V regulates cell cycle progression and histone stability in the nucleus of breast cancer cells. Front Pharmacol 14:1271435 (2023). PubMed: 38026973
- Bao H et al. NASP maintains histone H3-H4 homeostasis through two distinct H3 binding modes. Nucleic Acids Res 50:5349-5368 (2022). PubMed: 35489058
- Zhu Y et al. Knockdown of long noncoding RNA colorectal neoplasia differentially expressed inhibits hepatocellular carcinoma progression by mediating the expression of nuclear autoantigenic sperm protein. Oncol Rep 46:N/A (2021). PubMed: 34633056
- Kong F et al. MiR-381-3p suppresses biological characteristics of cancer in head-neck squamous cell carcinoma cells by targeting nuclear autoantigenic sperm protein (NASP). Biosci Biotechnol Biochem 84:703-713 (2020). PubMed: 31797734