Anti-NARG1抗体[434C2a] (ab60065)
![Western blot - Anti-NARG1 antibody [434C2a] (ab60065)](https://www.abcam.cn/ps/products/60/ab60065/Images/ab60065_WB_1.jpg "Western blot - Anti-NARG1 antibody [434C2a] (ab60065)")
Key features and details
- Mouse monoclonal [434C2a] to NARG1
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
概述
-
产品名称
Anti-NARG1抗体[434C2a]
参阅全部 NARG1 一抗 -
描述
小鼠单克隆抗体[434C2a] to NARG1 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Recombinant fragment -
免疫原
Recombinant fragment (Human)
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.0225% Potassium chloride, 0.03% Potassium phosphate, 0.1312% Sodium phosphate, 0.812% Sodium chloride, 1% BSA -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
Purified using protein G column chromatography, from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. -
克隆
单克隆 -
克隆编号
434C2a -
同种型
IgG1 -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab60065于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
| 说明 |
|---|
应用说明
Dot: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately 32 kDa for the recombinant protein fragment (predicted molecular weight: 101 kDa for the full length protein).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Detects a band of approximately 32 kDa for the recombinant protein fragment (predicted molecular weight: 101 kDa for the full length protein).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
-
功能
The NAA10-NAA15 complex displays alpha (N-terminal) acetyltransferase activity that may be important for vascular, hematopoietic and neuronal growth and development. Required to control retinal neovascularization in adult ocular endothelial cells. In complex with XRCC6 and XRCC5 (Ku80), up-regulates transcription from the osteocalcin promoter. -
组织特异性
Expressed at high levels in testis and in ocular endothelial cells. Also found in brain (corpus callosum), heart, colon, bone marrow and at lower levels in most adult tissues, including thyroid, liver, pancreas, mammary and salivary glands, lung, ovary, urogenital system and upper gastrointestinal tract. Overexpressed in gastric cancer, in papillary thyroid carcinomas and in a Burkitt lymphoma cell line (Daudi). Specifically suppressed in abnormal proliferating blood vessels in eyes of patients with proliferative diabetic retinopathy. -
序列相似性
Contains 8 TPR repeats. -
翻译后修饰
Cleaved by caspases during apoptosis, resulting in a stable 35 kDa fragment. -
细胞定位
Cytoplasm. Nucleus. Mainly cytoplasmic, nuclear in some cases. Present in the free cytosolic and cytoskeleton-bound polysomes, but not in the membrane-bound polysomes. - Information by UniProt
-
别名
- 5730450D16Rik antibody
- 6330400I15 antibody
- ASTBDN antibody
see all
图片
-
Western blot - Anti-NARG1 antibody [434C2a] (ab60065)Western blot analysis of immunized recombinant protein, using ab60065
数据表及文件
-
Datasheet download
文献 (3)
ab60065 被引用在 3 文献中.
- Cirinelli A et al. Evidence that the transcriptional repressor ICER is regulated via the N-end rule for ubiquitination. Exp Cell Res 414:113083 (2022). PubMed: 35227662
- Kweon HY et al. Naa12 compensates for Naa10 in mice in the amino-terminal acetylation pathway. Elife 10:N/A (2021). PubMed: 34355692
- Dörfel MJ et al. Proteomic and genomic characterization of a yeast model for Ogden syndrome. Yeast 34:19-37 (2017). WB . PubMed: 27668839
