Anti-NAPRT1抗体(ab127699)
Key features and details
- Rabbit polyclonal to NAPRT1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-NAPRT1抗体
参阅全部 NAPRT1 一抗 -
描述
兔多克隆抗体to NAPRT1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment, corresponding to a region within amino acids 107-408 of Human NAPRT1.
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阳性对照
- A549, HeLa, HepG2 and HCT116 whole cell lysates; Human lung carcinoma tissue, HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab127699于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/5000 - 1/20000. Predicted molecular weight: 58 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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说明 |
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WB
1/5000 - 1/20000. Predicted molecular weight: 58 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
Catalyzes the conversion of nicotinic acid (NA) to NA mononucleotide (NaMN). Essential for NA to increase cellular NAD levels and prevent oxidative stress of the cells. -
通路
Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1. -
序列相似性
Belongs to the NAPRTase family. -
细胞定位
Cytoplasm > cytosol. - Information by UniProt
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数据库链接
- Entrez Gene: 93100 Human
- Omim: 611552 Human
- SwissProt: Q6XQN6 Human
- Unigene: 493164 Human
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别名
- FHA HIT interacting protein antibody
- FHA-HIT-interacting protein antibody
- FHIP antibody
see all
图片
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Anti-NAPRT1 antibody (ab127699) at 1/10000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 58 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPRT1 antibody (ab127699)ab127699, at a dilution of 1/500, staining NAPRT1 in paraffin-embedded Human lung carcinoma tissue by Immunohistochemistry.
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ab127699, at a dilution of 1/500, staining NAPRT1 in methanol-fixed HeLa cells by Immunofluorescence (Green). Alpha-tubulin filaments were stained Red.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab127699 被引用在 2 文献中.
- Nomura M et al. Niacin restriction with NAMPT-inhibition is synthetic lethal to neuroendocrine carcinoma. Nat Commun 14:8095 (2023). PubMed: 38092728
- James EL et al. Replicatively senescent human fibroblasts reveal a distinct intracellular metabolic profile with alterations in NAD+ and nicotinamide metabolism. Sci Rep 6:38489 (2016). PubMed: 27924925