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重组RabMAb

重组Anti-NAP1L1抗体[EPR12619] (ab174836)

Submit a review Submit a question References (2)
Western blot - Anti-NAP1L1 antibody [EPR12619] (ab174836)
  • Flow Cytometry (Intracellular) - Anti-NAP1L1 antibody [EPR12619] (ab174836)
  • Anti-NAP1L1 antibody [EPR12619] (ab174836)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12619] to NAP1L1
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-NAP1L1抗体[EPR12619]
    参阅全部 NAP1L1 一抗

  • 描述

    兔单克隆抗体[EPR12619] to NAP1L1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WBmore details
    不适用于: ICC/IF,IHC-P or IP

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human NAP1L1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: P55209

  • 阳性对照

    • HeLa, Jurkat, 293T and A431 cell lysates; Jurkat cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab174836于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 52-60 kDa (predicted molecular weight: 45 kDa).
说明
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 52-60 kDa (predicted molecular weight: 45 kDa).
应用说明
Is unsuitable for ICC/IF,IHC-P or IP.

靶标

  • 功能

    May be involved in modulating chromatin formation and contribute to regulation of cell proliferation.

  • 组织特异性

    Ubiquitously expressed.

  • 序列相似性

    Belongs to the nucleosome assembly protein (NAP) family.

  • 结构域

    The acidic domains are probably involved in the interaction with histones.

  • 翻译后修饰

    Polyglutamylated by TTLL4, a modification that occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Some residues may also be monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human.

  • 细胞定位

    Nucleus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • FLJ16112 antibody
    • hNRP antibody
    • HSP22 like protein interacting protein antibody
    • MGC23410 antibody
    • MGC8688 antibody
    • NAP 1 antibody
    • NAP 1 related protein antibody
    • NAP 1L antibody
    • NAP-1-related protein antibody
    • NAP1 antibody
    • NAP1 L1 antibody
    • NAP1 related protein antibody
    • NAP1L antibody
    • Nap1l1 antibody
    • NAP1L1 protein antibody
    • NP1L1_HUMAN antibody
    • NRP antibody
    • Nucleosome assembly protein 1 like 1 antibody
    • Nucleosome assembly protein 1-like 1 antibody
    • Nucleosome assembly protein I-related protein antibody
    see all

图片

  • Western blot - Anti-NAP1L1 antibody [EPR12619] (ab174836)
    Western blot - Anti-NAP1L1 antibody [EPR12619] (ab174836)
    All lanes : Anti-NAP1L1 antibody [EPR12619] (ab174836) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 45 kDa

  • Flow Cytometry (Intracellular) - Anti-NAP1L1 antibody [EPR12619] (ab174836)
    Flow Cytometry (Intracellular) - Anti-NAP1L1 antibody [EPR12619] (ab174836)

    Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling NAP1L1with ab174836 at 1/10 dilution (red), or negative control rabbit IgG (green). 

     

     

  • Anti-NAP1L1 antibody [EPR12619] (ab174836)
    Anti-NAP1L1 antibody [EPR12619] (ab174836)

文献 (2)

发表研究结果有使用 ab174836？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab174836 被引用在 2 文献中.

  • Li T  et al. Nucleosome assembly protein 1 like 1 (NAP1L1) promotes cardiac fibrosis by inhibiting YAP1 ubiquitination and degradation. MedComm (2020) 4:e348 (2023). PubMed: 37593048
  • Geis FK  et al. CHAF1A/B mediate silencing of unintegrated HIV-1 DNAs early in infection. Proc Natl Acad Sci U S A 119:N/A (2022). PubMed: 35074917

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