重组Anti-NAP1L1抗体[EPR11845] (ab178687)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11845] to NAP1L1
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-NAP1L1抗体[EPR11845]
参阅全部 NAP1L1 一抗 -
描述
兔单克隆抗体[EPR11845] to NAP1L1 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IF, IHC-P, IPmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa, 293T, A431 and Jurkat whole cell lysate (ab7899). Human kidney tissue. HeLa cells. Permeabilized 293T cells. Immunoprecipitation pellet from 293T cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR11845 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab178687于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/10000 - 1/50000. Predicted molecular weight: 45 kDa.
|
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ICC/IF |
1/100 - 1/250.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
IP |
1/10 - 1/100.
|
说明 |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Predicted molecular weight: 45 kDa. |
ICC/IF
1/100 - 1/250. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
1/10 - 1/100. |
靶标
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功能
May be involved in modulating chromatin formation and contribute to regulation of cell proliferation. -
组织特异性
Ubiquitously expressed. -
序列相似性
Belongs to the nucleosome assembly protein (NAP) family. -
结构域
The acidic domains are probably involved in the interaction with histones. -
翻译后修饰
Polyglutamylated by TTLL4, a modification that occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Some residues may also be monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. -
细胞定位
Nucleus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 4673 Human
- Omim: 164060 Human
- SwissProt: P55209 Human
- Unigene: 524599 Human
- Unigene: 695185 Human
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别名
- FLJ16112 antibody
- hNRP antibody
- HSP22 like protein interacting protein antibody
see all
图片
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All lanes : Anti-NAP1L1 antibody [EPR11845] (ab178687) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 45 kDa -
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NAP1L1 with ab178687 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescence analysis of HeLa cells labeling NAP1L1 with ab178687 at a 1/100 dilution. DAPI nuclear staining (blue).
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Intracellular flow cytometric analysis of permeabilized 293T cells using ab178687 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).
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Western blot analysis on immunoprecipitation pellet from (Lane 1) 293T cell lysate or (Lane 2) 1X PBS (negative control) using ab178687 at a 1/10 dilution for IP and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab178687 被引用在 1 文献中.
- Yip MCJ et al. Mechanism of client selection by the protein quality-control factor UBE2O. Nat Struct Mol Biol 29:774-780 (2022). PubMed: 35915257