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AB178687

重组Anti-NAP1L1抗体[EPR11845]

Anti-NAP1L1 antibody [EPR11845]

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(1 Publication)

Rabbit Recombinant Monoclonal NAP1L1 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

查看别名

NRP, NAP1L1, Nucleosome assembly protein 1-like 1, NAP-1-related protein, hNRP

5 Images
Western blot - Anti-NAP1L1 antibody [EPR11845] (AB178687)
  • WB

Supplier Data

Western blot - Anti-NAP1L1 antibody [EPR11845] (AB178687)

All lanes:

Western blot - Anti-NAP1L1 antibody [EPR11845] (ab178687) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Predicted band size: 45 kDa

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAP1L1 antibody [EPR11845] (AB178687)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAP1L1 antibody [EPR11845] (AB178687)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NAP1L1 with ab178687 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-NAP1L1 antibody [EPR11845] (AB178687)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NAP1L1 antibody [EPR11845] (AB178687)

Immunofluorescence analysis of HeLa cells labeling NAP1L1 with ab178687 at a 1/100 dilution. DAPI nuclear staining (blue).

Immunoprecipitation - Anti-NAP1L1 antibody [EPR11845] (AB178687)
  • IP

Supplier Data

Immunoprecipitation - Anti-NAP1L1 antibody [EPR11845] (AB178687)

Western blot analysis on immunoprecipitation pellet from (Lane 1) 293T cell lysate or (Lane 2) 1X PBS (negative control) using ab178687 at a 1/10 dilution for IP and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-NAP1L1 antibody [EPR11845] (ab178687)

Predicted band size: 45 kDa

false

Flow Cytometry (Intracellular) - Anti-NAP1L1 antibody [EPR11845] (AB178687)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-NAP1L1 antibody [EPR11845] (AB178687)

Intracellular flow cytometric analysis of permeabilized 293T cells using ab178687 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

不同偶联物与剂型 (1)

  • Carrier free

    Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR11845

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

ICC/IF, IP, IHC-P, WB, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯度
Tissue culture supernatant
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

NAP1L1 also known as Nucleosome Assembly Protein 1-Like 1 plays a role mechanically by assisting in nucleosome assembly. This protein has a mass of about 45 kDa and is expressed in various tissues including the brain and liver. NAP1L1 moves within the nucleus and cytoplasm which allows interaction with DNA and other proteins involved in chromatin organization.
Biological function summary

NAP1L1 contributes to chromatin remodeling by facilitating the assembly and disassembly of nucleosomes. NAP1L1 forms part of a complex that modulates chromatin structure and function influencing gene expression and DNA replication. It interacts with histones contributing to the deposition and removal of histones H2A and H2B making it essential for proper cell cycle progression and regulation.

Pathways

NAP1L1 acts in key signaling interconnected with chromatin modification and cell cycle control. It participates in the histone chaperone pathways influencing chromatin structure dynamics. NAP1L1 associates with proteins like histone H2A-H2B and the chromatin assembly factor CAF-1. These proteins collectively maintain chromatin integrity and regulation during DNA replication and repair processes.

NAP1L1 has links to certain cancers and neurological disorders. Increased levels of NAP1L1 are observable in some tumor types suggesting a role in cancer progression. In neurological contexts alterations in this protein are noted in conditions like Alzheimer's disease where chromatin misregulation is apparent. Connections with other proteins such as histone variants may further implicate NAP1L1 in these pathological states.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Histone chaperone that plays a role in the nuclear import of H2A-H2B and nucleosome assembly (PubMed : 20002496, PubMed : 21211722, PubMed : 26841755). Participates also in several important DNA repair mechanisms : greatly enhances ERCC6-mediated chromatin remodeling which is essential for transcription-coupled nucleotide excision DNA repair (PubMed : 28369616). Stimulates also homologous recombination (HR) by RAD51 and RAD54 which is essential in mitotic DNA double strand break (DSB) repair (PubMed : 24798879). Plays a key role in the regulation of embryonic neurogenesis (By similarity). Promotes the proliferation of neural progenitors and inhibits neuronal differentiation during cortical development (By similarity). Regulates neurogenesis via the modulation of RASSF10; regulates RASSF10 expression by promoting SETD1A-mediated H3K4 methylation at the RASSF10 promoter (By similarity).. (Microbial infection) Positively regulates Epstein-Barr virus reactivation in epithelial cells through the induction of viral BZLF1 expression.. (Microbial infection) Together with human herpesvirus 8 protein LANA1, assists the proper assembly of the nucleosome on the replicated viral DNA.
See full target information NAP1L1
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nature structural & molecular biology 29:774-780 PubMed35915257

2022

Mechanism of client selection by the protein quality-control factor UBE2O.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew C J Yip,Samantha F Sedor,Sichen Shao
View all publications
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Abcam Product Promise

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详情请参阅我们的条款与条件。
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