Anti-NANOS2抗体(ab70000)
Key features and details
- Rabbit polyclonal to NANOS2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-NANOS2抗体 -
描述
兔多克隆抗体to NANOS2 -
宿主
Rabbit -
特异性
ab70000 does not cross-react with either Nanos1 or Nanos3. -
经测试应用
适用于: WBmore details
不适用于: IHC -
种属反应性
与反应: Human
预测可用于: Rat
-
免疫原
Synthetic peptide corresponding to Human NANOS2 (C terminal). 13 amino acid peptide near the carboxy terminus of the human NANOS2.
-
阳性对照
- WB: SK-N-SH cell lysate
-
常规说明
Previous batches of ab70000 have been successfully used in IHC-P on testis tissue sections (please see Kusz KM et al., 2009). However, when tested on human brain tissue sections, ab70000 gave negative results in IHC-P (data not shown). We do not guarantee the current batch of ab70000 to work in IHC.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70000于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
|
| 说明 |
|---|
|
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa). |
靶标
-
功能
Plays a key role in the sexual differentiation of germ cells by promoting the male fate but suppressing the female fate. Represses the female fate pathways by suppressing meiosis, which in turn results in the promotion of the male fate. Maintains the suppression of meiosis by preventing STRA8 expression, which is required for premeiotic DNA replication, after CYP26B1 is decreased. Regulates the localization of the CCR4-NOT deadenylation complex to P-bodies and plays a role in recruiting the complex to trigger the degradation of mRNAs involved in meiosis. Required for the maintenance of the spermatogonial stem cell population. Not essential for the assembly of P-bodies but is required for the maintenance of their normal state. -
组织特异性
Testis and ovary. Expression found in several spermatogenic stages: in cells on the periphery of the tubules which could correspond to spermatogonia, in spermatocytes and in round spermatids (at protein level). -
序列相似性
Belongs to the nanos family.
Contains 1 nanos-type zinc finger. -
发展阶段
Fetal ovary and fetal testis. Present in all germ cells of seminiferous tubules of the 24-week fetus (at protein level). -
结构域
The Nanos-type zinc finger is composed of two C2HC motifs, each motif binding one molecule of zinc. It is essential for the translation repression activity of the protein. -
细胞定位
Cytoplasm. Cytoplasm > P-body. Cytoplasm > perinuclear region. Localizes at P-bodies during gonocyte development (By similarity). More abundant in perinuclear region of the cytoplasm of the germ cells of the adult testis. - Information by UniProt
-
数据库链接
- Entrez Gene: 339345 Human
- Entrez Gene: 365213 Rat
- Omim: 608228 Human
- SwissProt: P60321 Human
- Unigene: 434218 Human
-
别名
- NANO2_HUMAN antibody
- Nanos homolog 2 antibody
- Nanos homolog 2 Drosophila antibody
see all
图片
-
Western blot - Anti-NANOS2 antibody (ab70000)Anti-NANOS2 antibody (ab70000) at 2 µg/ml + SK-N-SH cell lysate at 15 µg
Predicted band size: 15 kDa
Observed band size: 15 kDa
数据表及文件
-
SDS download
-
Datasheet download
文献 (4)
ab70000 被引用在 4 文献中.
- Lei JH et al. Cytotoxicity of nonylphenol on spermatogonial stem cells via phosphatidylinositol-3-kinase/protein kinase B/mammalian target of rapamycin pathway. World J Stem Cells 12:500-513 (2020). PubMed: 32742567
- Zhuang M et al. Reelin regulates male mouse reproductive capacity via the sertoli cells. J Cell Biochem 120:1174-1184 (2019). PubMed: 30335884
- Kong D et al. PKA regulatory IIa subunit is essential for PGD2-mediated resolution of inflammation. J Exp Med 213:2209-26 (2016). PubMed: 27621415
- Kusz KM et al. The highly conserved NANOS2 protein: testis-specific expression and significance for the human male reproduction. Mol Hum Reprod 15:165-71 (2009). WB, IHC-P ; Human . PubMed: 19168545