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Anti-NANOGP8抗体(ab74096)

Submit a review Q&A (2)References (1)
Western blot - Anti-NANOGP8 antibody (ab74096)

    Key features and details

    • Rabbit polyclonal to NANOGP8
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    概述

    • 产品名称

      Anti-NANOGP8抗体

    • 描述

      兔多克隆抗体to NANOGP8

    • 宿主

      Rabbit

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Synthesized peptide derived from an internal region of human NANOGP8.

    • 阳性对照

      • Extracts from Jurkat, HUVEC, HT-29 and HepG2 cells.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    • 存储溶液

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS

      Without Mg2+ and Ca2+
    • Concentration information loading...

    • 纯度

      Immunogen affinity purified

    • 纯化说明

      The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.

    • 克隆

      多克隆

    • 同种型

      IgG

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab74096于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    1/500 - 1/1000. Predicted molecular weight: 35 kDa.
    说明
    WB
    1/500 - 1/1000. Predicted molecular weight: 35 kDa.

    靶标

    • 相关性

      NANOGP8 may act as a transcription regulator. When overexpressed, promotes cells to enter into S phase and proliferation. Expressed in tumor uterine cervix, breast and urinary bladder tissues, and also osteosarcoma, hepatoma, and breast adenocarcinoma cancer cell lines.

    • 细胞定位

      Nuclear

    • 数据库链接

      • 别名

        • NANOG antibody
        • Nanog homeobox pseudogene 8 antibody
        • NANOGP1 antibody
        • NANOGP8 antibody

      图片

      • Western blot - Anti-NANOGP8 antibody (ab74096)
        Western blot - Anti-NANOGP8 antibody (ab74096)
        All lanes : Anti-NANOGP8 antibody (ab74096) at 1/500 dilution

        Lane 1 : Extracts from Jurkat cells with no peptide
        Lane 2 : Extracts from HUVEC cells with no peptide
        Lane 3 : Extracts from HT-29 cells with no peptide
        Lane 4 : Extracts from HepG2 cells with no peptide
        Lane 5 : Extracts from Jurkat cells with immunizing peptide at 10 µg

        Lysates/proteins at 30 µg per lane.

        Predicted band size: 35 kDa
        Observed band size: 35 kDa
        Additional bands at: 22 kDa. We are unsure as to the identity of these extra bands.

      文献 (1)

      发表研究结果有使用 ab74096？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab74096 被引用在 1 文献中.

      • Jiang Z  et al. Oncogenic NanogP8 expression regulates cell proliferation and migration through the Akt/mTOR signaling pathway in human gastric cancer - SGC-7901cell line. Onco Targets Ther 9:4859-66 (2016). PubMed: 27563247

      客户评价及客户问答

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      1-2 of 2 Abreviews or Q&A

      Question

      Is this antibody specific to nanogP8? Has cross reactivity/seqence homology  with nanog been tested?

      Read More
      Answer

      Thank you for your patience. The lab told me that they didn't test the antibody to check reactivity with Nanog. Thus, we do not have any data to prove that the antibody will not crossreact with Nanog.   Due to the high homology between both proteins it might be difficult to make an antibody that is exclusively specific to Nanogp8. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. 

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      Question

      Is this antibody specific to nanogP8? Has cross reactivity/seqence homology  with nanog been tested?

      Read More
      Answer

      Thank you for contacting us. I was in touch with the originating lab, and received the following information: "This antibody is specific to NANOGP8. The sequence homology is 92% with NANOG." I have asked them again if they have done any tests to exclude crossreactivity with Nanog since a 92% homology is quite high. I will let you know what I hear back. Thank you for your patience. In the meantime, please do not hesitate to contact us if you need any more advice or information.

      Read More

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