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重组RabMAb

重组Anti-Nanog抗体[RM1168] - BSA and Azide free (ab317507)

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Western blot - Anti-Nanog antibody [RM1168] - BSA and Azide free (ab317507)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [RM1168] - BSA and Azide free (ab317507)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [RM1168] - BSA and Azide free (ab317507)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [RM1168] - BSA and Azide free (ab317507)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [RM1168] - BSA and Azide free (ab317507)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [RM1168] - BSA and Azide free (ab317507)
  • Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [RM1168] - BSA and Azide free (ab317507)
  • Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [RM1168] - BSA and Azide free (ab317507)
  • Flow Cytometry (Intracellular) - Anti-Nanog antibody [RM1168] - BSA and Azide free (ab317507)
  • Immunoprecipitation - Anti-Nanog antibody [RM1168] - BSA and Azide free (ab317507)
  • Immunoprecipitation - Anti-Nanog antibody [RM1168] - BSA and Azide free (ab317507)
  • Anti-Nanog antibody [RM1168] - BSA and Azide free (ab317507)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit recombinant multiclonal [RM1168] to Nanog - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IP, ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-Nanog抗体[RM1168] - BSA and Azide free
    参阅全部 Nanog 一抗

  • 描述

    兔重组multiclonal [RM1168] to Nanog - BSA and Azide free

  • 宿主

    Rabbit

  • 特异性

    Unsuitable for mouse IHC-P and mouse FC-intra.

  • 经测试应用

    适用于: Flow Cyt (Intra), IP, ICC/IF, IHC-P, WBmore details

  • 种属反应性

    与反应: Mouse, Human
    不与反应: Rat

  • 免疫原

    This product was produced with the following immunogens:
    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: NCCIT, NT2/D1, F9, and ES-D3 whole cell lysates. IHC-P: Human dysgerminoma, Human embryonal carcinoma and Human seminoma tissues. ICC/IF: NCCIT and F9 cells. Flow Cyt (Intra): NCCIT cell. IP: ab317506 IP in NCCIT and F9 whole cell lysates.

  • 常规说明

    ab317507 is the carrier-free version of ab317506

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab317507于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration.
说明
Flow Cyt (Intra)
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration.

靶标

  • 功能

    Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.

  • 组织特异性

    Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.

  • 序列相似性

    Belongs to the Nanog homeobox family.
    Contains 1 homeobox DNA-binding domain.

  • 发展阶段

    Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Embryonic stem cell specific homeobox protein (Nanog) antibody
      • ENK antibody
      • FLJ12581 antibody
      • hNanog antibody
      • Homeobox protein NANOG antibody
      • Homeobox transcription factor Nanog antibody
      • homeobox transcription factor Nanog-delta 48 antibody
      • NANOG antibody
      • Nanog homeobox antibody
      • NANOG_HUMAN antibody
      see all

    图片

    • Western blot - Anti-Nanog antibody [RM1168] - BSA and Azide free (ab317507)
      Western blot - Anti-Nanog antibody [RM1168] - BSA and Azide free (ab317507)
      All lanes : Anti-Nanog antibody [RM1168] (ab317506) at 1/1000 dilution

      Lane 1 : NCCIT (human pluripotent embryonic carcinoma epithelial cell) whole cell lysate
      Lane 2 : NT2/D1 (human malignant pluripotent embryonic carcinoma epithelial-like cell) whole cell lysate
      Lane 3 : Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate
      Lane 4 : F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate
      Lane 5 : ES-D3 (mouse blastocyst-derived embryonic stem cell) whole cell lysate
      Lane 6 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Observed band size: 29-42 kDa why is the actual band size different from the predicted?



      This data was developed using ab317506, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Negative control: Jurkat, NIH/3T3 (PMID: 12787505)

      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

      Exposure time: Lane 1, 3, 4-6: 26 seconds Lane 2: 92 seconds

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [RM1168] - BSA and Azide free (ab317507)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [RM1168] - BSA and Azide free (ab317507)

      This data was developed using ab317506, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human dysgerminoma tissue labeling Nanog with ab317506 at 1/500 (0.998 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on human dysgerminoma. The section was incubated with ab317506 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [RM1168] - BSA and Azide free (ab317507)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [RM1168] - BSA and Azide free (ab317507)

      This data was developed using ab317506, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human embryonal carcinoma tissue labeling Nanog with ab317506 at 1/500 (0.998 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on human embryonal carcinoma. The section was incubated with ab317506 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [RM1168] - BSA and Azide free (ab317507)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [RM1168] - BSA and Azide free (ab317507)

      This data was developed using ab317506, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human seminoma tissue labeling Nanog with ab317506 at 1/500 (0.998 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on human seminoma. The section was incubated with ab317506 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [RM1168] - BSA and Azide free (ab317507)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [RM1168] - BSA and Azide free (ab317507)

      This data was developed using ab317506, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Nanog with ab317506 at 1/500 (0.998 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Negative control: no staining on human kidney. The section was incubated with ab317506 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [RM1168] - BSA and Azide free (ab317507)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [RM1168] - BSA and Azide free (ab317507)

      This data was developed using ab317506, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Nanog with ab317506 at 1/500 (0.998 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Negative control: no staining on human colon. The section was incubated with ab317506 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [RM1168] - BSA and Azide free (ab317507)
      Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [RM1168] - BSA and Azide free (ab317507)

      This data was developed using ab317506, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NCCIT (human pluripotent embryonic carcinoma epithelial cell) cells labelling Nanog with ab317506 at 1/100 (4.99 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

      Confocal image showing mainly nuclear staining in NCCIT cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
      Negative control: Jurkat (PMID: 12787505).
      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [RM1168] - BSA and Azide free (ab317507)
      Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [RM1168] - BSA and Azide free (ab317507)

      This data was developed using ab317506, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized F9 (mouse embryonal carcinoma epithelial cell) cells labelling Nanog with ab317506 at 1/100 (4.99 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

      Confocal image showing mainly nuclear staining in F9 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
      Negative control: NIH/3T3 (PMID: 12787505).
      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

    • Flow Cytometry (Intracellular) - Anti-Nanog antibody [RM1168] - BSA and Azide free (ab317507)
      Flow Cytometry (Intracellular) - Anti-Nanog antibody [RM1168] - BSA and Azide free (ab317507)

      This data was developed using ab317506, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Jurkat (human T cell leukemia T lymphocyte from peripheral blood, Left) / NCCIT (human pluripotent embryonic carcinoma epithelial cell, Right) cells labelling Nanog with ab317506 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

      Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

      Negative control: Jurkat.

    • Immunoprecipitation - Anti-Nanog antibody [RM1168] - BSA and Azide free (ab317507)
      Immunoprecipitation - Anti-Nanog antibody [RM1168] - BSA and Azide free (ab317507)

      This data was developed using ab317506, the same antibody clone in a different buffer formulation.

      Nanog was immunoprecipitated from 0.35 mg NCCIT (human pluripotent embryonic carcinoma epithelial cell) whole cell lysate with ab317506 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317506 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: NCCIT (human pluripotent embryonic carcinoma epithelial cell) whole cell lysate
      Lane 2: ab317506 IP in NCCIT (human pluripotent embryonic carcinoma epithelial cell) whole cell lysate
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab317506 in NCCIT whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 32 seconds.

    • Immunoprecipitation - Anti-Nanog antibody [RM1168] - BSA and Azide free (ab317507)
      Immunoprecipitation - Anti-Nanog antibody [RM1168] - BSA and Azide free (ab317507)

      This data was developed using ab317506, the same antibody clone in a different buffer formulation.

      Nanog was immunoprecipitated from 0.35 mg F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate with ab317506 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317506 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate
      Lane 2: ab317506 IP in F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab317506 in F9 whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 180 seconds.

    • Anti-Nanog antibody [RM1168] - BSA and Azide free (ab317507)
      Anti-Nanog antibody [RM1168] - BSA and Azide free (ab317507)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

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    ab317507 尚未被引用在任何文献中。

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