Anti-Nanog抗体(ab77095)
Key features and details
- Goat polyclonal to Nanog
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Nanog抗体
参阅全部 Nanog 一抗 -
描述
山羊多克隆抗体to Nanog -
宿主
Goat -
特异性
We have received reports this antibody does not work in pig, therefore we cannot guarantee it will work in this species. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Dog, Pig
不与反应:
Mouse -
免疫原
Synthetic peptide:
QNQRMKSKRWQKNN
by a Cysteine residue linker, corresponding to internal sequence amino acids 144-157 of Human Nanog -
阳性对照
- Induced pluriform stem cells derived from human keratinocytes. Human ovary lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab77095于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 35 kDa).
A 1 hour primary incubation is recommended for this product. ab77095 is not suitable for Western Blot with pig samples. |
| 说明 |
|---|
|
WB
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 35 kDa). A 1 hour primary incubation is recommended for this product. ab77095 is not suitable for Western Blot with pig samples. |
靶标
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功能
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation. -
组织特异性
Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. -
序列相似性
Belongs to the Nanog homeobox family.
Contains 1 homeobox DNA-binding domain. -
发展阶段
Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 100293888 Human
- Entrez Gene: 79923 Human
- Omim: 607937 Human
- SwissProt: Q9H9S0 Human
- Unigene: 635882 Human
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别名
- Embryonic stem cell specific homeobox protein (Nanog) antibody
- ENK antibody
- FLJ12581 antibody
see all
图片
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Western blot - Anti-Nanog antibody (ab77095)Anti-Nanog antibody (ab77095) at 0.1 µg/ml + Human ovary lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 35 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab77095 被引用在 13 文献中.
- Kanegi R et al. Generation, characterization, and differentiation of induced pluripotent stem-like cells in the domestic cat. J Reprod Dev 69:317-327 (2023). PubMed: 37880086
- Recchia K et al. In vitro induced pluripotency from urine-derived cells in porcine. World J Stem Cells 14:231-244 (2022). PubMed: 35432738
- Ramos SA et al. Generation of functional human thymic cells from induced pluripotent stem cells. J Allergy Clin Immunol 149:767-781.e6 (2022). PubMed: 34331993
- Wi H et al. Immunosuppression-enhancing effect of the administration of allogeneic canine adipose-derived mesenchymal stem cells (cA-MSCs) compared with autologous cA-MSCs in vitro. J Vet Sci 22:e63 (2021). PubMed: 34423601
- Trujillo CA et al. Pharmacological reversal of synaptic and network pathology in human MECP2-KO neurons and cortical organoids. EMBO Mol Med 13:e12523 (2021). PubMed: 33501759