Anti-Nanog抗体(ab21624)
Key features and details
- Rabbit polyclonal to Nanog
- Suitable for: ICC/IF, WB, Sandwich ELISA
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
概述
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产品名称
Anti-Nanog抗体
参阅全部 Nanog 一抗 -
描述
兔多克隆抗体to Nanog -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, Sandwich ELISAmore details -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Synthetic peptide corresponding to Human Nanog aa 29-49.
Sequence:CGPEENYPSLQMSSAEMPHTE
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常规说明
Concentration of Anti-Nanog antibody: 0.2 mg/mL
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab21624于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (10) |
1/1000.
|
| WB | (3) |
1/200. Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa).
Some users report a stronger signal after blocking and diluting antibodies with 1% BSA instead of 5% milk. Mouse target cannot be detected by WB. |
| Sandwich ELISA |
Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [1E6C4] to Nanog (ab76586) as Capture.
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| 说明 |
|---|
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ICC/IF
1/1000. |
|
WB
1/200. Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa). Some users report a stronger signal after blocking and diluting antibodies with 1% BSA instead of 5% milk. Mouse target cannot be detected by WB. |
|
Sandwich ELISA
Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [1E6C4] to Nanog (ab76586) as Capture. |
靶标
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功能
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation. -
组织特异性
Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. -
序列相似性
Belongs to the Nanog homeobox family.
Contains 1 homeobox DNA-binding domain. -
发展阶段
Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 100293888 Human
- Entrez Gene: 79923 Human
- Omim: 607937 Human
- SwissProt: Q9H9S0 Human
- Unigene: 635882 Human
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别名
- Embryonic stem cell specific homeobox protein (Nanog) antibody
- ENK antibody
- FLJ12581 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody (ab21624)ab21624 at a 1/1000 dilution staining Nanog in human embryonic stem cells by ICC. An antibody towards Oct4, which has an identical staining pattern to ab21624, was used as a positive control.
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Western blot - Anti-Nanog antibody (ab21624)All lanes : Anti-Nanog antibody (ab21624) at 1/200 dilution
Lane 1 : Human fetal fibroblast lysate
Lane 2 : Human iPS cell lysate
Predicted band size: 34 kDa -
Western blot - Anti-Nanog antibody (ab21624)All lanes : Anti-Nanog antibody (ab21624) at 1/1000 dilution
Lane 1 : whole cell extract of mouse 3T3 cells expressing mouse Nanog.
Lane 2 : whole cell extract of mouse 3T3 cells expressing human Nanog.
Predicted band size: 34 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
ab21624 recognizes human Nanog but does not recognize mouse Nanog in Western Blot. -
Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody (ab21624)Image courtesy of Mick WauchopeImmunocytochemical analysis of Human iPSCs labeling Nanog with ab21624 at 1/75 dilution.
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Sandwich ELISA - Anti-Nanog antibody (ab21624)Standard Curve for Nanog (Analyte: Nanog protein (ab50053)); dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [1E6C4] to Nanog (ab76586) at 0.2 ug/ml and Detector Antibody Rabbit polyclonal to Nanog - ChIP Grade (ab21624) at 0.5 ug/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (282)
ab21624 被引用在 282 文献中.
- Feng X et al. Ligand-free mitochondria-localized mutant AR-induced cytotoxicity in spinal bulbar muscular atrophy. Brain 146:278-294 (2023). PubMed: 35867854
- He Y et al. 4E-BP1 counteracts human mesenchymal stem cell senescence via maintaining mitochondrial homeostasis. Protein Cell 14:202-216 (2023). PubMed: 36929036
- Akter M et al. Generation of two induced pluripotent stem cell lines with heterozygous and homozygous amyotrophic lateral sclerosis-causing mutation R521G (c.1561C > G) in FUS gene. Stem Cell Res 69:103078 (2023). PubMed: 36965406
- Yang J et al. TRIM29 facilitates proliferation and malignancy of cholangiocarcinoma cells by activating MAPK and β-catenin pathways. Environ Toxicol 38:2002-2010 (2023). PubMed: 37219039
- Liu X et al. Allele-specific gene-editing approach for vision loss restoration in RHO-associated retinitis pigmentosa. Elife 12:N/A (2023). PubMed: 37272616