Anti-Nanog 抗体
Anti-Nanog antibody
5
(16 Reviews)
|
(282 Publications)
Anti-Nanog antibody (ab21624) is a rabbit polyclonal antibody detecting Nanog in Western Blot, ICC/IF, ELISA. Suitable for Human,.
- Over 280 publications
- Trusted since 2005
查看别名
Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog, NANOG
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody (AB21624)
ab21624 at a 1/1000 dilution staining Nanog in human embryonic stem cells by ICC. An antibody towards Oct4, which has an identical staining pattern to ab21624, was used as a positive control.
- WB
Supplier Data
Western blot - Anti-Nanog antibody (AB21624)
All lanes:
Western blot - Anti-Nanog antibody (ab21624) at 1/200 dilution
Lane 1:
Human fetal fibroblast lysate
Lane 2:
Human iPS cell lysate
Predicted band size: 34 kDa
false
- ICC/IF
AbReview53716****
Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody (AB21624)
Immunocytochemical analysis of Human iPSCs labeling Nanog with ab21624 at 1/75 dilution.
Image courtesy of Mick Wauchope
- sELISA
Unknown
Sandwich ELISA - Anti-Nanog antibody (AB21624)
Standard Curve for Nanog (Analyte : Nanog protein (ab50053)); dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [1E6C4] to Nanog (ab76586) at 0.2 ug/ml and Detector Antibody Rabbit polyclonal to Nanog - ChIP Grade (ab21624) at 0.5 ug/ml.
- WB
Unknown
Western blot - Anti-Nanog antibody (AB21624)
ab21624 recognizes human Nanog but does not recognize mouse Nanog in Western Blot.
All lanes:
Western blot - Anti-Nanog antibody (ab21624) at 1/1000 dilution
Lane 1:
whole cell extract of mouse 3T3 cells expressing mouse Nanog.
Lane 2:
whole cell extract of mouse 3T3 cells expressing human Nanog.
Predicted band size: 34 kDa
Observed band size: 38 kDa
false
反应性数据
产品详情
Anti-Nanog antibody (ab21624) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Human, samples.
What is the molecular weight of Nanog?
Anti-Nanog (ab21624) specifically detects a band for Nanog (UniProt: Q9H9S0) at a molecular weight of 34kDa.
Trusted by the scientific community
Anti-Nanog (ab21624) was first used in a scientific publication in 2005 and has been cited over 280 times in peer-reviewed journals.
Reviewed by scientists
Anti-Nanog (ab21624) has over 15 independent reviews from customers.
性能和储存信息
形式
纯化工艺
存储溶液
运输条件
推荐的短期储存时间
推荐的短期储存条件
推荐的长期储存条件
分装信息
储存信息
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Nanog plays a central role in stem cell biology operating as part of a complex regulatory network. Nanog interacts with other important transcription factors like Oct4 and Sox2 forming a core pluripotency network. This network suppresses the differentiation of stem cells by regulating the expression of genes involved in cell fate decisions. By modulating the expression of different pathways Nanog ensures the maintenance of an undifferentiated state in stem cells.
Pathways
Nanog is deeply embedded in the Wnt/Β-Catenin and TGF-Β signaling pathways important for stem cell maintenance and differentiation. In the Wnt/Β-Catenin pathway Nanog works alongside proteins like Β-catenin to drive the expression of genes that promote self-renewal. Meanwhile in the TGF-Β pathway Nanog acts with proteins such as Smad2/3 to balance pluripotency and differentiation signals. These pathways critically support the complex network that sustains stem cell identity and function.
产品实验方案
- Visit the General protocols
- Visit the Troubleshooting
靶点信息
文献 (282)
Recent publications for all applications. Explore the full list and refine your search
Biology direct 18:80 PubMed38008751
2023
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Metabolites 13: PubMed37755263
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Nature 620:863-872 PubMed37587336
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International journal of molecular sciences 24: PubMed37628781
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EMBO reports 24:e56439 PubMed37306027
2023
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eLife 12: PubMed37272616
2023
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Bioactive materials 28:206-226 PubMed37274446
2023
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Environmental toxicology 38:2002-2010 PubMed37219039
2023
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Stem cell research 69:103078 PubMed36965406
2023
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Protein & cell 14:202-216 PubMed36929036
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Abcam Product Promise
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