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AB21624

Anti-Nanog 抗体

Anti-Nanog antibody

5

(16 Reviews)

|

(282 Publications)

Anti-Nanog antibody (ab21624) is a rabbit polyclonal antibody detecting Nanog in Western Blot, ICC/IF, ELISA. Suitable for Human,.

- Over 280 publications
- Trusted since 2005

查看别名

Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog, NANOG

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody (AB21624)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody (AB21624)

ab21624 at a 1/1000 dilution staining Nanog in human embryonic stem cells by ICC. An antibody towards Oct4, which has an identical staining pattern to ab21624, was used as a positive control.

Western blot - Anti-Nanog antibody (AB21624)
  • WB

Supplier Data

Western blot - Anti-Nanog antibody (AB21624)

All lanes:

Western blot - Anti-Nanog antibody (ab21624) at 1/200 dilution

Lane 1:

Human fetal fibroblast lysate

Lane 2:

Human iPS cell lysate

Predicted band size: 34 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody (AB21624)
  • ICC/IF

AbReview53716****

Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody (AB21624)

Immunocytochemical analysis of Human iPSCs labeling Nanog with ab21624 at 1/75 dilution.

Image courtesy of Mick Wauchope

Sandwich ELISA - Anti-Nanog antibody (AB21624)
  • sELISA

Unknown

Sandwich ELISA - Anti-Nanog antibody (AB21624)

Standard Curve for Nanog (Analyte : Nanog protein (ab50053)); dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [1E6C4] to Nanog (ab76586) at 0.2 ug/ml and Detector Antibody Rabbit polyclonal to Nanog - ChIP Grade (ab21624) at 0.5 ug/ml.

Western blot - Anti-Nanog antibody (AB21624)
  • WB

Unknown

Western blot - Anti-Nanog antibody (AB21624)

ab21624 recognizes human Nanog but does not recognize mouse Nanog in Western Blot.

All lanes:

Western blot - Anti-Nanog antibody (ab21624) at 1/1000 dilution

Lane 1:

whole cell extract of mouse 3T3 cells expressing mouse Nanog.

Lane 2:

whole cell extract of mouse 3T3 cells expressing human Nanog.

Predicted band size: 34 kDa

Observed band size: 38 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

sELISA, WB, ICC/IF

applications

免疫原

Synthetic Peptide within Human NANOG aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9H9S0

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200", "WB-species-notes": "<p>Some users report a stronger signal after blocking and diluting antibodies with 1% BSA instead of 5% milk. Mouse target cannot be detected by WB.</p>", "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p></p>" }, "Synthetic peptide - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "0.5 µg/mL", "sELISA-species-notes": "<p>For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [1E6C4] to Nanog (<a href='/products/unavailable/nanog-antibody-1e6c4-ab76586'>ab76586</a>) as Capture.</p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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为什么推荐这个?

我们推荐这个产品,因为它经常被用于相同的实验或相关的研究。

我们建议您务必查阅产品说明书,以确认其是否符合您的实验需求;若有疑问,也可联系我们的技术团队获取协助。

产品详情

What is this antibody validated in?
Anti-Nanog antibody (ab21624) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Human, samples.

What is the molecular weight of Nanog?
Anti-Nanog (ab21624) specifically detects a band for Nanog (UniProt: Q9H9S0) at a molecular weight of 34kDa.

Trusted by the scientific community
Anti-Nanog (ab21624) was first used in a scientific publication in 2005 and has been cited over 280 times in peer-reviewed journals.

Reviewed by scientists
Anti-Nanog (ab21624) has over 15 independent reviews from customers.

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7.2 Preservative: 0.1% Sodium azide Constituents: PBS
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Nanog also known as Nanog homeobox is a transcription factor playing an important role in maintaining the pluripotency of embryonic stem cells. The molecular weight of Nanog is approximately 35 kDa. It is found mainly in the inner cell mass of the blastocyst but it also expresses in embryonic stem cells and some adult stem cell populations. Nanog acts by binding to DNA in a sequence-specific manner to regulate gene expression essential for stem cell self-renewal and pluripotency.
Biological function summary

Nanog plays a central role in stem cell biology operating as part of a complex regulatory network. Nanog interacts with other important transcription factors like Oct4 and Sox2 forming a core pluripotency network. This network suppresses the differentiation of stem cells by regulating the expression of genes involved in cell fate decisions. By modulating the expression of different pathways Nanog ensures the maintenance of an undifferentiated state in stem cells.

Pathways

Nanog is deeply embedded in the Wnt/Β-Catenin and TGF-Β signaling pathways important for stem cell maintenance and differentiation. In the Wnt/Β-Catenin pathway Nanog works alongside proteins like Β-catenin to drive the expression of genes that promote self-renewal. Meanwhile in the TGF-Β pathway Nanog acts with proteins such as Smad2/3 to balance pluripotency and differentiation signals. These pathways critically support the complex network that sustains stem cell identity and function.

Nanog's expression relates closely to its role in various cancers such as glioblastoma and colorectal cancer. Abnormally high levels of Nanog contribute to the tumorigenicity of cancer cells by maintaining their self-renewal and undifferentiated state similar to its role in stem cells. Nanog interacts with proteins like p53 known for its tumor suppressor functions often leading to challenges in cancer treatment. Understanding Nanog's influence in these pathways provides valuable insights into therapeutic targets for combating cancer stem cell resilience.

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (PubMed : 25825768). Able to autorepress its expression in differentiating (ES) cells : binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
See full target information NANOG

文献 (282)

Recent publications for all applications. Explore the full list and refine your search

Biology direct 18:80 PubMed38008751

2023

RCC2 promotes prostate cancer cell proliferation and migration through Hh/GLI1 signaling pathway and cancer stem-like cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shenghan Wang,Zhentao Lei,Wei Liu,Jie Xiong,Yuqiang Shi,Lin Yang,Qiang Gao,Kai Le,Bao Zhang

Metabolites 13: PubMed37755263

2023

Perturbations of Glutathione and Sphingosine Metabolites in Port Wine Birthmark Patient-Derived Induced Pluripotent Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Vi Nguyen,Jacob Kravitz,Chao Gao,Marcelo L Hochman,Dehao Meng,Dongbao Chen,Yunguan Wang,Anil G Jegga,J Stuart Nelson,Wenbin Tan

Nature 620:863-872 PubMed37587336

2023

Transient naive reprogramming corrects hiPS cells functionally and epigenetically.

Applications

Unspecified application

Species

Unspecified reactive species

Sam Buckberry,Xiaodong Liu,Daniel Poppe,Jia Ping Tan,Guizhi Sun,Joseph Chen,Trung Viet Nguyen,Alex de Mendoza,Jahnvi Pflueger,Thomas Frazer,Dulce B Vargas-Landín,Jacob M Paynter,Nathan Smits,Ning Liu,John F Ouyang,Fernando J Rossello,Hun S Chy,Owen J L Rackham,Andrew L Laslett,James Breen,Geoffrey J Faulkner,Christian M Nefzger,Jose M Polo,Ryan Lister

International journal of molecular sciences 24: PubMed37628781

2023

A Novel Genetic Variant in Associated with Severe Epilepsy and Intellectual Disability: Potential Implications on Neural Primary Cilia.

Applications

Unspecified application

Species

Unspecified reactive species

Mariana Martins,Ana Rafaela Oliveira,Solange Martins,José Pedro Vieira,Pedro Perdigão,Ana Rita Fernandes,Luís Pereira de Almeida,Paulo Jorge Palma,Diana Bela Sequeira,João Miguel Marques Santos,Frederico Duque,Guiomar Oliveira,Ana Luísa Cardoso,João Peça,Catarina Morais Seabra

EMBO reports 24:e56439 PubMed37306027

2023

Reducing oxidative protein folding alleviates senescence by minimizing ER-to-nucleus H O release.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Cheng,Qianzhao Ji,Lu Wang,Chih-Chen Wang,Guang-Hui Liu,Lei Wang

eLife 12: PubMed37272616

2023

Allele-specific gene-editing approach for vision loss restoration in -associated retinitis pigmentosa.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaozhen Liu,Jing Qiao,Ruixuan Jia,Fan Zhang,Xiang Meng,Yang Li,Liping Yang

Bioactive materials 28:206-226 PubMed37274446

2023

Dual human iPSC-derived cardiac lineage cell-seeding extracellular matrix patches promote regeneration and long-term repair of infarcted hearts.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Jiang,Ling-Ling Zhang,Fan Zhang,Wei Bi,Peng Zhang,Xiu-Jian Yu,Sen-Le Rao,Shi-Hui Wang,Qiang Li,Chen Ding,Yin Jin,Zhong-Min Liu,Huang-Tian Yang

Environmental toxicology 38:2002-2010 PubMed37219039

2023

TRIM29 facilitates proliferation and malignancy of cholangiocarcinoma cells by activating MAPK and β-catenin pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Yang,Wenqing Bao,Haiyan Diao,Shaofei Yang,Lin Qiu,Chunjing Xu,Bin Zhao

Stem cell research 69:103078 PubMed36965406

2023

Generation of two induced pluripotent stem cell lines with heterozygous and homozygous amyotrophic lateral sclerosis-causing mutation R521G (c.1561C > G) in FUS gene.

Applications

Unspecified application

Species

Unspecified reactive species

Masuma Akter,Haochen Cui,Md Abir Hosain,Baojin Ding

Protein & cell 14:202-216 PubMed36929036

2023

4E-BP1 counteracts human mesenchymal stem cell senescence via maintaining mitochondrial homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Yifang He,Qianzhao Ji,Zeming Wu,Yusheng Cai,Jian Yin,Yiyuan Zhang,Sheng Zhang,Xiaoqian Liu,Weiqi Zhang,Guang-Hui Liu,Si Wang,Moshi Song,Jing Qu
View all publications

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