Name: Anti-Nanog antibody [23D2-3C6]
SKU: ab173368
Rating: 5 (2 reviews)

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Western blot - Anti-Nanog antibody [23D2-3C6] (ab173368)

Key features and details

Mouse monoclonal [23D2-3C6] to Nanog
Suitable for: WB, ICC/IF, Flow Cyt
Reacts with: Human
Isotype: IgG

产品名称

Anti-Nanog抗体[23D2-3C6]
参阅全部 Nanog 一抗
描述

小鼠单克隆抗体[23D2-3C6] to Nanog
宿主

Mouse
特异性

ab173368 detects an ~38 kDa protein in embryonal carcinoma cells. Subcellular fractionation shows nuclear localization of Nanog. It shows specificity to Nanog and is non-reactive to lysates from non-embryonal cell types (e.g. HeLa cell lysate).
经测试应用

适用于: WB, ICC/IF, Flow Cytmore details
种属反应性

与反应: Human
预测可用于: Chimpanzee, Cynomolgus monkey
免疫原

Recombinant full length protein corresponding to Human Nanog aa 1-305. expressed in bacteria.
Sequence:
MSVDPACPQS LPCFEASDCK ESSPMPVICG PEENYPSLQM SSAEMPHTET VSPLPSSMDL LIQDSPDSST SPKGKQPTSA EKSVAKKEDK VPVKKQKTRT VFSSTQLCVL NDRFQRQKYL SLQQMQELSN ILNLSYKQVK TWFQNQRMKS KRWQKNNWPK NSNGVTQKAS APTYPSLYSS YHQGCLVNPT GNLPMWSNQT WNNSTWSNQT QNIQSWSNHS WNTQTWCTQS WNNQAWNSPF YNCGEESLQS CMQFQPNSPA SDLEAALEAA GEGLNVIQQT TRYFSTPQTM DLFLNYSMNM QPEDV

Database link: Q9H9S0
Run BLAST with Run BLAST with
阳性对照
- NTERA-2 cells; HEL 11.4 induced IPS cells; H9 embryonic stem cells. NCCIT cells.
常规说明

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液

Preservative: 0.05% Sodium azide
Constituents: 30% Glycerol, 0.1% BSA, 69% PBS
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

23D2-3C6
同种型

IgG
研究领域

Compatible Secondaries
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Isotype control
- Mouse IgG - Isotype Control (ab37355)
Recombinant Protein
- Recombinant human Nanog protein (ab134886)
Related Products
- Recombinant human Nanog protein (ab134886)
- Recombinant Human Nanog protein (ab50053)

The Abpromise guarantee

Abpromise™承诺保证使用ab173368于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
WB		1/500 - 1/2000. Predicted molecular weight: 34 kDa.
ICC/IF	(1)	1/50 - 1/100.
Flow Cyt		1/100.

说明
WB 1/500 - 1/2000. Predicted molecular weight: 34 kDa.
ICC/IF 1/50 - 1/100.
Flow Cyt 1/100.

功能

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
组织特异性

Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
序列相似性

Belongs to the Nanog homeobox family.
Contains 1 homeobox DNA-binding domain.
发展阶段

Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
细胞定位

Nucleus.
Target information above from: UniProt accession Q9H9S0 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 100293888 Human
- Entrez Gene: 79923 Human
- Omim: 607937 Human
- SwissProt: Q9H9S0 Human
- Unigene: 635882 Human
别名
- Embryonic stem cell specific homeobox protein (Nanog) antibody
- ENK antibody
- FLJ12581 antibody
- hNanog antibody
- Homeobox protein NANOG antibody
- Homeobox transcription factor Nanog antibody
- homeobox transcription factor Nanog-delta 48 antibody
- NANOG antibody
- Nanog homeobox antibody
- NANOG_HUMAN antibody
see all

Western blot - Anti-Nanog antibody [23D2-3C6] (ab173368)

All lanes : Anti-Nanog antibody [23D2-3C6] (ab173368) at 1/1000 dilution

Lane 1 : NTERA-2 cell line
Lane 2 : NCCIT cell line
Lane 3 : iPSC cell line
Lane 4 : iPSC differentiated to definitive endoderm
Lane 5 : HeLa cell line

Lysates/proteins at 30 µg per lane.

Secondary
All lanes : Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP at 1/40000 dilution

Predicted band size: 34 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [23D2-3C6] (ab173368)

Immunofluorescent analysis of H9 embryonic stem cells grown for a few days on Matrigel-coated chamber slides (4% paraformaldehyde fixed and permeabilized with 0.1% Triton X-100) labeling Nanog with ab173368 at 1/200 dilution (green) followed by a fluorescein-conjugated secondary antibody at 1/100 dilution. Nuclei (blue) were stained with DAPI.
Western blot - Anti-Nanog antibody [23D2-3C6] (ab173368)

All lanes : Anti-Nanog antibody [23D2-3C6] (ab173368) at 1/1000 dilution

Lane 1 : NCCIT nuclear fraction lysate
Lane 2 : NCCIT cytoplasmic fraction lysate

Lysates/proteins at 60 µg per lane.

Secondary
All lanes : goat anti-mouse IgG-HRP at 1/20000 dilution

Developed using the ECL technique.

Predicted band size: 34 kDa

4-20% Tris-HCl polyacrylamide gel.
Western blot - Anti-Nanog antibody [23D2-3C6] (ab173368)

All lanes : Anti-Nanog antibody [23D2-3C6] (ab173368) at 1/1000 dilution

Lane 1 : NCCIT lysate
Lane 2 : NTERA-2 lysate
Lane 3 : HeLa lysate

Lysates/proteins at 60 µg per lane.

Secondary
All lanes : goat anti-mouse IgG-HRP at 1/20000 dilution

Developed using the ECL technique.

Predicted band size: 34 kDa

4-20% Tris-HCl polyacrylamide gel
Flow Cytometry - Anti-Nanog antibody [23D2-3C6] (ab173368)

Flow cytometric analysis of H9 embryonic stem cells labeling Nanog with ab173368 at 1/100 (blue histogram) or mouse IgG (green histogram) followed by a fluorescein-conjugated secondary at 1/200 dilution.
Flow Cytometry - Anti-Nanog antibody [23D2-3C6] (ab173368)

Flow cytometric analysis of HEL 11.4 induced IPS cells labeling Nanog with ab173368 at 1/00 dilution (blue histogram) or mouse IgG (green histogram) followed by a fluorescein-conjugated secondary antibody at 1/200 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [23D2-3C6] (ab173368)

Immunofluorescent analysis of HEL 11.4 induced IPS cells grown for a few days on Matrigel-coated chamber slides (4% paraformaldehyde fixed and permeabilized with 0.1% Triton X-100) labeling Nanog with ab173368 at 1/200 dilution (green) followed by a fluorescein-conjugated secondary antibody at 1/100 dilution. Nuclei (blue) were stained with DAPI.
Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [23D2-3C6] (ab173368)

Immunofluorescent analysis of NTERA-2 and HeLa cells (formalin-fixed and permeabilized with 0.1% Triton X-100) labeling Nanog with ab173368 at 1/50 dilution (green) followed by DyLight 488-conjugated goat anti-mouse IgG secondary antibody. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.

Flow cytometry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

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发表研究结果有使用 ab173368？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab173368 被引用在 7 文献中.

Fang Y et al. Expanding Embedded 3D Bioprinting Capability for Engineering Complex Organs with Freeform Vascular Networks. Adv Mater 35:e2205082 (2023). PubMed: 36796025
Goyal D et al. Vascular Stem Cells and the Role of B-Raf Kinase in Survival, Proliferation, and Apoptosis. Int J Mol Sci 24:N/A (2023). PubMed: 37108645
Grunwald V et al. Development of Zika Virus E Variants for Pseudotyping Retroviral Vectors Targeting Glioblastoma Cells. Int J Mol Sci 24:N/A (2023). PubMed: 37833934
Bai W et al. LINC00589-dominated ceRNA networks regulate multiple chemoresistance and cancer stem cell-like properties in HER2+ breast cancer. NPJ Breast Cancer 8:115 (2022). PubMed: 36309503
Veldhuizen J et al. Modeling long QT syndrome type 2 on-a-chip via in-depth assessment of isogenic gene-edited 3D cardiac tissues. Sci Adv 8:eabq6720 (2022). PubMed: 36525500
Biswas P et al. Detection and validation of novel mutations in MERTK in a simplex case of retinal degeneration using WGS and hiPSC-RPEs model. Hum Mutat 42:189-199 (2021). PubMed: 33252167
Fang D et al. Mfn2 is Required for Mitochondrial Development and Synapse Formation in Human Induced Pluripotent Stem Cells/hiPSC Derived Cortical Neurons. Sci Rep 6:31462 (2016). PubMed: 27535796

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (hiPSC)

Permeabilization

Yes - PBS + 0.1% Triton X-100

Specification

hiPSC

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Gabriel Galea

Verified customer

提交于 Feb 05 2021

Application

IHC - Wholemount

Sample

Mouse Embryo (Pharyngeal arches)

Specification

Pharyngeal arches

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Gabriel Galea

Verified customer

提交于 Dec 15 2020

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Key features and details

概述

产品名称

描述

宿主

特异性

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Compatible Secondaries

Isotype control

Recombinant Protein

Related Products

应用

The Abpromise guarantee

靶标

功能

组织特异性

序列相似性

发展阶段

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (7)

客户评价及客户问答

Immunocytochemistry/ Immunofluorescence abreview for Anti-Nanog antibody [23D2-3C6]

IHC - Wholemount abreview for Anti-Nanog antibody [23D2-3C6]