AB109735

Anti-NAK/TBK1 抗体 [EPR2867(2)-19]

Name: NAK/TBK1抗体[EPR2867(2)-19] (ab109735)| Abcam中文官网
Brand: Abcam Limited
SKU: AB109735
Price: 1845 CNY
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Anti-NAK/TBK1 antibody [EPR2867(2)-19]

20ul selling size
RabMAb
Recombinant
KO Validated
了解详情

(1 Review)

(23 Publications)

Rabbit Recombinant Monoclonal NAK/TBK1 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 23 publications.

查看别名

NAK, TBK1, Serine/threonine-protein kinase TBK1, NF-kappa-B-activating kinase, T2K, TANK-binding kinase 1

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAK/TBK1 antibody [EPR2867(2)-19] (AB109735)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAK/TBK1 antibody [EPR2867(2)-19] (AB109735)

ab109735 staining NAK/TBK1 in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/800). An undiluted HRP-conjugated anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin.

Supplier Data

Immunoprecipitation - Anti-NAK/TBK1 antibody [EPR2867(2)-19] (AB109735)

Immunoprecipitation of TBK1 in HeLa cells. Lysates were prepared and immunoprecipitation was performed using 1.0 μg of ab109735 pre-coupled to prot.A-Sepharose beads. Samples were washed and processed for western blot with ab12116 at 1 in 2000. This data was kindly provided by the YCharOS Inc., an open science company with the mission of characterizing every commercially available antibody reagent. Abcam are working with YCharOS to support their mission of antibody characterisation using knock out cell lines.

All lanes:

Immunoprecipitation - Anti-NAK/TBK1 antibody [EPR2867(2)-19] (ab109735)

Predicted band size: 84 kDa

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Immunoprecipitation - Anti-NAK/TBK1 antibody [EPR2867(2)-19] (AB109735)

Purified ab109735 at 1/30 dilution (2μg) immunoprecipitating NAK/TBK1 in HeLa whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
Lane 2 (+) : ab109735 + HeLa whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab109735 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/5000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 84 kDa

All lanes:

Immunoprecipitation - Anti-NAK/TBK1 antibody [EPR2867(2)-19] (ab109735)

Predicted band size: 61 kDa,84 kDa,94 kDa

Observed band size: 85 kDa

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Collaborator

Western blot - Anti-NAK/TBK1 antibody [EPR2867(2)-19] (AB109735)

ab109735 was shown to react with TBK1 in wild-type U2OSn cells in Western blot with loss of signal observed in a TBK1 knockout cell line. Wild-type U2OSn and TBK1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab109735 overnight at 4 °C at a 1/10000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 1/5000 before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-NAK/TBK1 antibody [EPR2867(2)-19] (ab109735)

Predicted band size: 84 kDa

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Lab

Western blot - Anti-NAK/TBK1 antibody [EPR2867(2)-19] (AB109735)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : NAK/TBK1 knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : HepG2 cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab109735 observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab109735 was shown to recognize NAK/TBK1 when NAK/TBK1 knockout samples were used, along with additional cross-reactive bands. Wild-type and NAK/TBK1 knockout samples were subjected to SDS-PAGE. ab109735 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-NAK/TBK1 antibody [EPR2867(2)-19] (ab109735)

Predicted band size: 84 kDa

false

Unknown

Western blot - Anti-NAK/TBK1 antibody [EPR2867(2)-19] (AB109735)

All lanes:

Western blot - Anti-NAK/TBK1 antibody [EPR2867(2)-19] (ab109735) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

U937 cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Predicted band size: 84 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-NAK/TBK1 antibody [EPR2867(2)-19] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR2867(2)-19

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P, IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

This antibody may have weak cross-reactivity with IKBKE (IKKε).

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/90", "FlowCyt-species-notes": "For unpurified use at 1/50.<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "For unpurified use at 1/1000 - 1/2000.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/800", "IHCP-species-notes": "For unpurified use at 1/400. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The NAK/TBK1 also known as TANK-binding kinase 1 is a fundamental protein kinase in various cellular processes. It weighs approximately 84 kDa and is expressed in many tissues including lymphoid organs and the brain. NAK acts as a serine/threonine-protein kinase which transfers a phosphate group to specific substrates modulating their function and activity. Phosphorylation by TBK1 initiates several signaling events making it an important regulator in immune responses and inflammatory signaling pathways.

Biological function summary

This kinase plays a significant role in antiviral defense and autophagy. NAK/TBK1 is also involved in innate immune signaling and cell survival. TBK1 forms part of a larger signaling complex interacting with adaptors like TRAF3 and TANK which are important for its proper localization and function. Phospho-NAK its phosphorylated form can activate other molecules driving various downstream immune and stress responses.

Pathways

The protein is integral to the type I interferon signaling pathway and NF-kB pathway. NAK/TBK1 interacts closely with IRF3 a transcription factor important for antiviral activity and plays a role alongside other proteins like IKKε. These interactions permit activation of genes necessary for defense mechanisms against viral pathogens and control of inflammation. In the NF-kB pathway TBK1 helps regulate cellular responses to stress and inflammatory signals.

NAK/TBK1 has associations with amyotrophic lateral sclerosis (ALS) and various cancers. Research shows connections between TBK1 mutations and ALS indicating its importance in neuronal function and health. Additionally aberrations in TBK1 activity contribute to oncogenesis as it can affect cell proliferation and survival. The protein's relation with signaling proteins such as p62/SQSTM1 highlights its diverse role in disease mechanisms making it a potential therapeutic target.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Serine/threonine kinase that plays an essential role in regulating inflammatory responses to foreign agents (PubMed : 10581243, PubMed : 11839743, PubMed : 12692549, PubMed : 12702806, PubMed : 14703513, PubMed : 15367631, PubMed : 15485837, PubMed : 18583960, PubMed : 21138416, PubMed : 23453971, PubMed : 23453972, PubMed : 23746807, PubMed : 25636800, PubMed : 26611359, PubMed : 32404352, PubMed : 34363755). Following activation of toll-like receptors by viral or bacterial components, associates with TRAF3 and TANK and phosphorylates interferon regulatory factors (IRFs) IRF3 and IRF7 as well as DDX3X (PubMed : 12692549, PubMed : 12702806, PubMed : 14703513, PubMed : 15367631, PubMed : 18583960, PubMed : 25636800). This activity allows subsequent homodimerization and nuclear translocation of the IRFs leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNA and IFNB (PubMed : 12702806, PubMed : 15367631, PubMed : 25636800, PubMed : 32972995). In order to establish such an antiviral state, TBK1 form several different complexes whose composition depends on the type of cell and cellular stimuli (PubMed : 23453971, PubMed : 23453972, PubMed : 23746807). Plays a key role in IRF3 activation : acts by first phosphorylating innate adapter proteins MAVS, STING1 and TICAM1 on their pLxIS motif, leading to recruitment of IRF3, thereby licensing IRF3 for phosphorylation by TBK1 (PubMed : 25636800, PubMed : 30842653). Phosphorylated IRF3 dissociates from the adapter proteins, dimerizes, and then enters the nucleus to induce expression of interferons (PubMed : 25636800). Thus, several scaffolding molecules including FADD, TRADD, MAVS, AZI2, TANK or TBKBP1/SINTBAD can be recruited to the TBK1-containing-complexes (PubMed : 21931631). Under particular conditions, functions as a NF-kappa-B effector by phosphorylating NF-kappa-B inhibitor alpha/NFKBIA, IKBKB or RELA to translocate NF-Kappa-B to the nucleus (PubMed : 10783893, PubMed : 15489227). Restricts bacterial proliferation by phosphorylating the autophagy receptor OPTN/Optineurin on 'Ser-177', thus enhancing LC3 binding affinity and antibacterial autophagy (PubMed : 21617041). Phosphorylates SMCR8 component of the C9orf72-SMCR8 complex, promoting autophagosome maturation (PubMed : 27103069). Phosphorylates ATG8 proteins MAP1LC3C and GABARAPL2, thereby preventing their delipidation and premature removal from nascent autophagosomes (PubMed : 31709703). Phosphorylates and activates AKT1 (PubMed : 21464307). Seems to play a role in energy balance regulation by sustaining a state of chronic, low-grade inflammation in obesity, wich leads to a negative impact on insulin sensitivity (By similarity). Attenuates retroviral budding by phosphorylating the endosomal sorting complex required for transport-I (ESCRT-I) subunit VPS37C (PubMed : 21270402). Phosphorylates Borna disease virus (BDV) P protein (PubMed : 16155125). Plays an essential role in the TLR3- and IFN-dependent control of herpes virus HSV-1 and HSV-2 infections in the central nervous system (PubMed : 22851595). Acts both as a positive and negative regulator of the mTORC1 complex, depending on the context : activates mTORC1 in response to growth factors by catalyzing phosphorylation of MTOR, while it limits the mTORC1 complex by promoting phosphorylation of RPTOR (PubMed : 29150432, PubMed : 31530866). Involved in the regulation of TNF-induced RIPK1-mediated cell death, probably acting via CYLD phosphorylation that in turn controls RIPK1 ubiquitination status (PubMed : 34363755). Participates also in the differentiation of T follicular regulatory cells together with the receptor ICOS (PubMed : 27135603).

See full target information TBK1

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文献 (23)

Recent publications for all applications. Explore the full list and refine your search

Cell biology and toxicology 41:82 PubMed40335763

2025

ELF4 improves sepsis-induced myocardial injury by regulating STING signaling-mediated T cells differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Yawen Zheng,Xiongjun Peng,Yusha Zhang,Ruilin Liu,Junke Long

Cell death discovery 11:98 PubMed40075110

2025

TBK1 is involved in programmed cell death and ALS-related pathways in novel zebrafish models.

Applications

Unspecified application

Species

Unspecified reactive species

Quentin Raas,Gregoire Haouy,Hortense de Calbiac,Elena Pasho,Anca Marian,Ida Chiara Guerrera,Marion Rosello,Patrick Oeckl,Filippo Del Bene,Alberto Catanese,Sorana Ciura,Edor Kabashi

The Journal of biological chemistry 299:105428 PubMed37926288

2023

TBK1-stabilized ZNF268a recruits SETD4 to methylate TBK1 for efficient interferon signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Liu,Wei Yin,Xianhuang Zeng,Jinhao Fan,Chaozhi Liu,Mingyu Gao,Zan Huang,Guihong Sun,Mingxiong Guo

PLoS pathogens 19:e1011742 PubMed37871014

2023

Tyrosine phosphorylation of IRF3 by BLK facilitates its sufficient activation and innate antiviral response.

Applications

Unspecified application

Species

Unspecified reactive species

Wei-Wei Li,Xu-Xu Fan,Zi-Xiang Zhu,Xue-Jing Cao,Zhao-Yu Zhu,Dan-Shi Pei,Yi-Zhuo Wang,Ji-Yan Zhang,Yan-Yi Wang,Hai-Xue Zheng

Scientific reports 13:14405 PubMed37658135

2023

The transcription factor NRF1 (NFE2L1) activates aggrephagy by inducing p62 and GABARAPL1 after proteasome inhibition to maintain proteostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Atsushi Hatanaka,Sota Nakada,Gen Matsumoto,Katsuya Satoh,Iori Aketa,Akira Watanabe,Tomoaki Hirakawa,Tadayuki Tsujita,Tsuyoshi Waku,Akira Kobayashi

Journal of immunology research 2023:5990156 PubMed37032653

2023

c-Myb Dominates TBK1-Mediated Endotoxin Tolerance in Kupffer Cells by Negatively Regulating DTX4.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Lin Wu,Le-Han Pan,Zhu-Jun Yi,Wen-Feng Zhang,Jian-Ping Gong

PLoS pathogens 18:e1010989 PubMed36449507

2022

MITA oligomerization upon viral infection is dependent on its N-glycosylation mediated by DDOST.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Tu,Xiu-Juan Yin,Qian Liu,Shan Zhang,Jie Wang,Ben-Zhe Ji,Jie Zhang,Ming-Shun Sun,Yang Yang,Chen-Hui Wang,Lei Yin,Yu Liu

F1000Research 11:977 PubMed36415206

2022

Identification of highly specific antibodies for Serine/threonine-protein kinase TBK1 for use in immunoblot, immunoprecipitation and immunofluorescence.

Applications

Unspecified application

Species

Unspecified reactive species

Walaa Alshafie,Maryam Fotouhi,Irina Shlaifer,Riham Ayoubi,Aled M Edwards,Thomas M Durcan,Peter S McPherson,Carl Laflamme

Journal of virology 94: PubMed32238587

2020

Human Cytomegalovirus Protein UL94 Targets MITA to Evade the Antiviral Immune Response.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Mei Zou,Zhe-Fu Huang,Yan Yang,Wei-Wei Luo,Su-Yun Wang,Min-Hua Luo,Yu-Zhi Fu,Yan-Yi Wang

Neuropathology and applied neurobiology 46:279-291 PubMed31498468

2019

Neuropathological characterization of a novel TANK binding kinase (TBK1) gene loss of function mutation associated with amyotrophic lateral sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

M Weinreich,S R Shepheard,N Verber,M Wyles,P R Heath,J R Highley,J Kirby,P J Shaw

View all publications

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Anti-NAK/TBK1 抗体 [EPR2867(2)-19]