Anti-N4BP1抗体(ab106649)
Key features and details
- Rabbit polyclonal to N4BP1
- Suitable for: ICC, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-N4BP1抗体
参阅全部 N4BP1 一抗 -
描述
兔多克隆抗体to N4BP1 -
宿主
Rabbit -
特异性
At least three isoforms of N4BP1 are known to exist; ab106649 will only detect the longest isoform. -
经测试应用
适用于: ICC, WBmore details -
种属反应性
与反应: Human -
免疫原
An 18 amino acid synthetic peptide near the N terminus of Human N4BP1 (NP_694574).
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阳性对照
- WB: Jurkat and K562 whole cell lysates; ICC: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at 4°C (stable for up to 12 months). -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab106649于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC |
Use a concentration of 10 - 20 µg/ml.
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| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 100 kDa.
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| 说明 |
|---|
|
ICC
Use a concentration of 10 - 20 µg/ml. |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 100 kDa. |
靶标
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功能
Inhibitor of the E3 ubiquitin-protein ligase ITCH. Acts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates. -
组织特异性
Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary. -
序列相似性
Belongs to the N4BP1 family. -
翻译后修饰
Monoubiquitinated by NEDD4. Polyubiquitinated, leading to its degradation by the proteasome. Sumoylated by SUMO1, abrogating polyubiquitination and subsequent degradation. Desumoylated by SENP1, leading to accumulation in PML nuclear bodies.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus > nucleolus. Nucleus > PML body. Primarily localizes to the nucleolus. Also localizes to the PML nuclear bodies, when desumoylated. - Information by UniProt
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数据库链接
- Entrez Gene: 9683 Human
- SwissProt: O75113 Human
- Unigene: 511839 Human
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别名
- FLJ31821 antibody
- KIAA0615 antibody
- MGC176730 antibody
see all
图片
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Western blot - Anti-N4BP1 antibody (ab106649)All lanes : Anti-N4BP1 antibody (ab106649) at 1 µg/ml
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 100 kDa
Exposure time: 30 secondsPrimary incubation for 1 hour at room temperature in 5% NFDM/TBST.
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Immunocytochemistry - Anti-N4BP1 antibody (ab106649)Immunofluorescence of N4BP1 in HeLa cells with ab106649 at 20 ug/mL. -
Immunocytochemistry - Anti-N4BP1 antibody (ab106649)Immunocytochemistry of N4BP1 in HeLa cells with ab106649 at 10 ug/mL.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab106649 被引用在 1 文献中.
- Pieters T et al. Cyclin D2 overexpression drives B1a-derived MCL-like lymphoma in mice. J Exp Med 218:N/A (2021). PubMed: 34406363