Anti-N WASP (phospho Y256)抗体(ab23395)
Key features and details
- Rabbit polyclonal to N WASP (phospho Y256)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-N WASP (phospho Y256)抗体
参阅全部 N WASP 一抗 -
描述
兔多克隆抗体to N WASP (phospho Y256) -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human N WASP (phospho Y256).
-
阳性对照
- Pervanadate treated A431, HeLa, Jurkat and endothelial cells for SDS PAGE immunoblots.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
This antibody was cross absorbed to phosphotyrosine then affinity purified using phospho N WASP (Tyr 256) peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab23395于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 55 kDa). The antibody detects a 63 or 65 kDa protein corresponding to phosphorylated N WASP on SDS PAGE immunoblots of A431, HeLa, Jurkat and endothelial cells treated with pervanadate. Weak bands are also observed at higher molecular weights after pervanadte treatment which may be due to low crossreactivity with phosphotyrosine.
|
| 说明 |
|---|
|
WB
1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 55 kDa). The antibody detects a 63 or 65 kDa protein corresponding to phosphorylated N WASP on SDS PAGE immunoblots of A431, HeLa, Jurkat and endothelial cells treated with pervanadate. Weak bands are also observed at higher molecular weights after pervanadte treatment which may be due to low crossreactivity with phosphotyrosine. |
靶标
-
功能
Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression. -
序列相似性
Contains 1 CRIB domain.
Contains 1 WH1 domain.
Contains 2 WH2 domains. -
细胞定位
Cytoplasm > cytoskeleton. Nucleus. Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated. - Information by UniProt
-
数据库链接
- Entrez Gene: 8976 Human
- Omim: 605056 Human
- SwissProt: O00401 Human
- Unigene: 143728 Human
- Unigene: 83916 Human
-
别名
- DKFZp779G0847 antibody
- MGC48327 antibody
- N-WASP antibody
see all
图片
-
Western blot - Anti-N WASP (phospho Y256) antibody (ab23395)All lanes : Anti-N WASP (phospho Y256) antibody (ab23395) at 1/10000 dilution
Lane 1 : Control A431 cells 20ug/lane
Lane 2 : Pervanadate-treated A431 cells 20 ug/lane
Predicted band size: 55 kDa
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
数据表及文件
-
SDS download
-
Datasheet download
文献 (6)
ab23395 被引用在 6 文献中.
- Du G et al. Targeting Src family kinase member Fyn by Saracatinib attenuated liver fibrosis in vitro and in vivo. Cell Death Dis 11:118 (2020). PubMed: 32051399
- Zhang W et al. Rho kinase collaborates with p21-activated kinase to regulate actin polymerization and contraction in airway smooth muscle. J Physiol 596:3617-3635 (2018). PubMed: 29746010
- Zhang W et al. p21-Activated kinase (Pak) regulates airway smooth muscle contraction by regulating paxillin complexes that mediate actin polymerization. J Physiol 594:4879-900 (2016). PubMed: 27038336
- Rajput C et al. Neural Wiskott-Aldrich syndrome protein (N-WASP)-mediated p120-catenin interaction with Arp2-Actin complex stabilizes endothelial adherens junctions. J Biol Chem 288:4241-50 (2013). WB ; Human . PubMed: 23212915
- Chacón MR et al. Focal adhesion kinase regulates actin nucleation and neuronal filopodia formation during axonal growth. Development 139:3200-10 (2012). WB . PubMed: 22872087
- Zhang W et al. The small GTPase RhoA regulates the contraction of smooth muscle tissues by catalyzing the assembly of cytoskeletal signaling complexes at membrane adhesion sites. J Biol Chem 287:33996-4008 (2012). WB ; Dog . PubMed: 22893699