Anti-N Cadherin (phospho Y820)抗体(ab76059)
Key features and details
- Rabbit polyclonal to N Cadherin (phospho Y820)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-N Cadherin (phospho Y820)抗体
参阅全部 N Cadherin 一抗 -
描述
兔多克隆抗体to N Cadherin (phospho Y820) -
宿主
Rabbit -
特异性
ab76059 detects a 130 kDa band corresponding to N cadherin in western blots of serum-starved human endothelial cells treated with pervanadate, but it is not detected in untreated cells. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human N Cadherin (phospho Y820).
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阳性对照
- Human endothelial cells treated with pervanadate (1 mM) for 30 min.
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常规说明
Do not aliquot this antibody.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab76059 was cross-adsorbed to phospho-N Cadherin (Tyr-860) and unphosphorylated N cadherin (Tyr-820) peptides before affinity purification using phospho-N cadherin (Tyr-820) peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76059于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 100 kDa.
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| 说明 |
|---|
|
WB
1/1000. Predicted molecular weight: 100 kDa. |
靶标
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功能
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. -
序列相似性
Contains 5 cadherin domains. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1000 Human
- Entrez Gene: 12558 Mouse
- Entrez Gene: 83501 Rat
- Omim: 114020 Human
- SwissProt: P19022 Human
- SwissProt: P15116 Mouse
- SwissProt: Q9Z1Y3 Rat
- Unigene: 464829 Human
see all -
别名
- CADH2_HUMAN antibody
- Cadherin 2 antibody
- Cadherin 2 N cadherin neuronal antibody
see all
图片
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Western blot - Anti-N Cadherin (phospho Y820) antibody (ab76059)All lanes : Anti-N Cadherin (phospho Y820) antibody (ab76059) at 1/1000 dilution
Lane 1 : Human endothelial cells untreated
Lane 2 : Human endothelial cells treated with pervanadate (1 mM) for 30 min
Lane 3 : Human endothelial cells treated with pervanadate (1 mM) for 30 min with phospho-N-cadherin (Tyr-820) peptide
Lane 4 : Human endothelial cells treated with pervanadate (1 mM) for 30 min with phospho-N-cadherin (Tyr-860) peptide
Predicted band size: 100 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab76059 被引用在 3 文献中.
- Zhang J et al. Identification and validation of an eight-lncRNA signature that predicts prognosis in patients with esophageal squamous cell carcinoma. Cell Mol Biol Lett 27:39 (2022). PubMed: 35578166
- Li Y et al. Apogossypolone Inhibits Cell Proliferation and Epithelial-Mesenchymal Transition in Cervical Cancer via Activating DKK3. Front Oncol 12:948023 (2022). PubMed: 35924156
- Chen C et al. Long non-coding RNA TMCC1-AS1 predicts poor prognosis and accelerates epithelial-mesenchymal transition in liver cancer. Oncol Lett 22:773 (2021). PubMed: 34589152