重组Anti-N Cadherin抗体[SP90] - C-terminal (ab225719)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)](https://www.abcam.cn/ps/products/225/ab225719/Images/ab225719-13-AntiN-Cadherin-antibody-SP90--Cterminal-IHC-Rat-liver.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP90] to N Cadherin - C-terminal
- Suitable for: Flow Cyt (Intra), IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-N Cadherin抗体[SP90] - C-terminal
参阅全部 N Cadherin 一抗 -
描述
兔单克隆抗体[SP90] to N Cadherin - C-terminal -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Cow
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Human mesothelioma, liver, adrenal gland fallopian tube, renal cell carcinoma, stomach, Human heart, Mouse liver, and Rat liver tissue; Flow Cyt (Intra): HeLa cells.
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常规说明
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
纯度
Protein A/G purified -
纯化说明
Purified from TCS by protein A/G. -
克隆
单克隆 -
克隆编号
SP90 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab225719于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| IHC-P |
1/100.
Antigen retrieval: Boil tissue section in 1mM EDTA, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes. |
| 说明 |
|---|
|
Flow Cyt (Intra)
Use at an assay dependent concentration. |
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IHC-P
1/100. Antigen retrieval: Boil tissue section in 1mM EDTA, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes. |
靶标
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功能
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. -
序列相似性
Contains 5 cadherin domains. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 281062 Cow
- Entrez Gene: 1000 Human
- Entrez Gene: 12558 Mouse
- Entrez Gene: 83501 Rat
- Omim: 114020 Human
- SwissProt: P19022 Human
- SwissProt: P15116 Mouse
- SwissProt: Q9Z1Y3 Rat
see all -
别名
- CADH2_HUMAN antibody
- Cadherin 2 antibody
- Cadherin 2 N cadherin neuronal antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat liver tissue sections labeling N Cadherin with Purified ab225719 at 1/100 dilution (0.56 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)](https://www.abcam.cn/ps/products/225/ab225719/Images/ab225719-12-AntiN-Cadherin-antibody-SP90--Cterminal-IHC-Mouse-liver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse liver tissue sections labeling N Cadherin with Purified ab225719 at 1/100 dilution (0.56 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)](https://www.abcam.cn/ps/products/225/ab225719/Images/ab225719-11-AntiN-Cadherin-antibody-SP90--Cterminal-IHC-Human-heart.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human heart tissue sections labeling N Cadherin with Purified ab225719 at 1/100 dilution (0.56 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)](https://www.abcam.cn/ps/products/225/ab225719/Images/ab225719-10-AntiN-Cadherin-antibody-SP90--Cterminal-IHC-Human-liver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling N Cadherin with Purified ab225719 at 1/100 dilution (0.56 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
![Flow Cytometry (Intracellular) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)](https://www.abcam.cn/ps/products/225/ab225719/Images/ab225719-330586-anti-n-cadherin-antibody-sp90-c-terminal-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)Flow cytometry analysis of Hela (human cervix adenocarcinoma) labeling N Cadherin with purified ab225719 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlableled control - Unlabelled cells (blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)](https://www.abcam.cn/ps/products/225/ab225719/Images/ab225719-289915-anti-n-cadherin-antibody-sp90-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)Formalin-fixed, paraffin-embedded human mesothelioma tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.
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![Flow Cytometry (Intracellular) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)](https://www.abcam.cn/ps/products/225/ab225719/Images/ab225719-289923-anti-n-cadherin-antibody-sp90-c-terminal-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling N Cadherin with ab225719 (green) compared with an isotype control rabbit IgG (blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)](https://www.abcam.cn/ps/products/225/ab225719/Images/ab225719-289916-anti-n-cadherin-antibody-sp90-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)Formalin-fixed, paraffin-embedded human mesothelioma tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)](https://www.abcam.cn/ps/products/225/ab225719/Images/ab225719-289917-anti-n-cadherin-antibody-sp90-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)Formalin-fixed, paraffin-embedded human liver tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)](https://www.abcam.cn/ps/products/225/ab225719/Images/ab225719-289918-anti-n-cadherin-antibody-sp90-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)Formalin-fixed, paraffin-embedded human adrenal gland tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)](https://www.abcam.cn/ps/products/225/ab225719/Images/ab225719-289919-anti-n-cadherin-antibody-sp90-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)Formalin-fixed, paraffin-embedded human fallopian tube tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)](https://www.abcam.cn/ps/products/225/ab225719/Images/ab225719-289920-anti-n-cadherin-antibody-sp90-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)](https://www.abcam.cn/ps/products/225/ab225719/Images/ab225719-289922-anti-n-cadherin-antibody-sp90-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)Formalin-fixed, paraffin-embedded human stomach tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.
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![Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)](https://www.abcam.cn/ps/products/225/ab225719/Images/ab225719-14-benefits-of-recombinant-antibodies.png)
Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab225719 被引用在 1 文献中.
- Khadri FZ et al. Impact of Epithelial-Mesenchymal Transition on the Immune Landscape in Breast Cancer. Cancers (Basel) 13:N/A (2021). PubMed: 34680248