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Anti-N Cadherin抗体[8C11] (ab19348)

Reviews (1)Q&A (3)References (27)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [8C11] (ab19348)
  • Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody [8C11] (ab19348)
  • Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody [8C11] (ab19348)

Key features and details

  • Mouse monoclonal [8C11] to N Cadherin
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Human, Bird
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Biotin PE

概述

  • 产品名称

    Anti-N Cadherin抗体[8C11]
    参阅全部 N Cadherin 一抗

  • 描述

    小鼠单克隆抗体[8C11] to N Cadherin

  • 宿主

    Mouse

  • 经测试应用

    适用于: ICC/IF, IHC-Pmore details

  • 种属反应性

    与反应: Human, Bird
    预测可用于: Rabbit, Hamster不与反应: Mouse, Rat, Cow, Pig

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 表位

    The 8C11 monoclonal binds to the extracellular domain of N-cadherin between EC3 and EC4 (PubMed ID: 12604612).

  • 阳性对照

    • IHC-P: Normal human heart tissue sections. ICC/IF: SH-SY5Y cells.

  • 常规说明

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab19348于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Use a concentration of 5 µg/ml.
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
说明
ICC/IF
Use a concentration of 5 µg/ml.
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能

    Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.

  • 序列相似性

    Contains 5 cadherin domains.

  • 细胞定位

    Cell membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • CADH2_HUMAN antibody
      • Cadherin 2 antibody
      • Cadherin 2 N cadherin neuronal antibody
      • Cadherin 2 type 1 antibody
      • Cadherin 2 type 1 N cadherin neuronal antibody
      • Cadherin 2, type 1, N-cadherin (neuronal) antibody
      • Cadherin-2 antibody
      • Cadherin2 antibody
      • Calcium dependent adhesion protein neuronal antibody
      • CD325 antibody
      • CD325 antigen antibody
      • CDH2 antibody
      • CDHN antibody
      • CDw325 antibody
      • CDw325 antigen antibody
      • N cadherin 1 antibody
      • N-cadherin antibody
      • NCAD antibody
      • Neural cadherin antibody
      • OTTHUMP00000066304 antibody
      • OTTHUMP00000067378 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [8C11] (ab19348)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [8C11] (ab19348)

      IHC image of N cadherin staining in a section of formalin-fixed paraffin-embedded normal human heart performed on a Leica BONDTM system using the standard protocol F.

      The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19348, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

      The inset secondary-only control image is taken from an identical assay without primary antibody.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody [8C11] (ab19348)
      Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody [8C11] (ab19348)

      ab19348 staining N-Cadherin in SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells.

      The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Tween for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab19348 at 5 μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150084, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labeled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody [8C11] (ab19348)
      Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody [8C11] (ab19348)Image from Chen HC et al., PLoS One. 2012;7(5):e36864. Epub 2012 May 9. Fig 1.; doi: 10.1371/journal.pone.0036864; May 9, 2012, PLoS One. 2012; 7(5): e36864. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Immunofluorescence analysis of ARPE-19 (human retinal pigment epithelial) cells, staining N Cadherin (green) with ab19348, at 1/20 dilution.

      ARPE-19 monolayer cultures were fixed in paraformaldehyde, permeabilized with 0.2% Triton X-100 for 15 min and blocked with 2% BSA for 30 min. Samples were incubated with primary antibody for 16 hours at 4°C before incubation with an Alexa Fluor® 488-conjugated donkey anti-mouse secondary IgG for 60 min.

    文献 (27)

    发表研究结果有使用 ab19348？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab19348 被引用在 27 文献中.

    • Han F  et al. Nano-calcium silicate mineralized fish scale scaffolds for enhancing tendon-bone healing. Bioact Mater 20:29-40 (2023). PubMed: 35633872
    • Yang W  et al. ZNF582 overexpression restrains the progression of clear cell renal cell carcinoma by enhancing the binding of TJP2 and ERK2 and inhibiting ERK2 phosphorylation. Cell Death Dis 14:212 (2023). PubMed: 36966163
    • Chai S  et al. Identification of epithelial and mesenchymal circulating tumor cells in clonal lineage of an aggressive prostate cancer case. NPJ Precis Oncol 6:41 (2022). PubMed: 35729213
    • Yang W  et al. Claudin-10 overexpression suppresses human clear cell renal cell carcinoma growth and metastasis by regulating ATP5O and causing mitochondrial dysfunction. Int J Biol Sci 18:2329-2344 (2022). PubMed: 35414767
    • Yang W  et al. Downregulation of lncRNA APCDD1L-AS1 due to DNA hypermethylation and loss of VHL protein expression promotes the progression of clear cell renal cell carcinoma. Int J Biol Sci 18:2583-2596 (2022). PubMed: 35414787
    View all Publications for this product

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    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-N Cadherin antibody [8C11]

     Inconclusive
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Xenopus laevis Tissue lysate - whole (Eye)
    Loading amount
    20 µg
    Specification
    Eye
    Gel Running Conditions
    Reduced Denaturing (8% SDS PAGE)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jul 07 2009

    Question

    Could you tell me the sequences used to raise ab76011 (EPR1791-4) and ab19348 (8C11), both of which are anti-N-Cadherin antibodies.

    Thankyou

    Read More
    Answer

    Thank you for contacting us and your interest in our products.
    The anti-N Cadherin antibody [8C11], ab19348, was raised against a bacterial fusion protein corresponding to the extracellular domain of N-cadherin (a domain between EC3 and EC4, amino acid residues 92-593 from human N-cadherin).
    The anti-N Cadherin antibody [EPR1791-4], ab76011, meanwhile was raised against a synthetic peptide taken from within the residues 100-200 of human N-cadherin , also within the extracellular domain of the protein.
    I hope this information has been of help. If you have any further questions, please do not hesitate to contact us again.

    Read More

    Question

    I AM LOOKING FOR AN ANTIBODY FOR WESTERN BLOTTING THAT REACTS WITH THE EXTRACELLULAR DOMAIN OF HUMAN N-CADHERIN. COULD YOU LET ME KNOW WHERE THE AB66025 BINDS. THANKYOU

    Read More
    Answer

    Thank you for your inquiry.

    The immunogen of ab66025 is the full length protein and to our knowledge this clone was not mapped. We therefore do not know where exactly this antibody binds to within the N Cadherin.

    According to SwissProt homepage the extracellular domain is aa 160-724.

    We have two antibodies that will bind within this sequence and that are tested and guaranteed for WB with human samples:


    ab19348
    https://www.abcam.com/N-Cadherin-antibody-8C11-ab19348.html https://www.abcam.com/N-Cadherin-antibody-8C11-ab19348.html.

    ab95440
    https://www.abcam.com/N-Cadherin-antibody-ab95440.html https://www.abcam.com/N-Cadherin-antibody-ab95440.html.

    I hope this information is helpful. Please do not hesitate to contact me again with any further questions.

    Read More

    Question

    Will ab19348 and ab167 cross-react with E- or N- cadherin, respectively?

    Read More
    Answer

    Thank you for your enquiry. Here is the link to the Clustal W alignment and I will also paste the alignment below. http://www.ebi.ac.uk/cgi-bin/clustalw/result?tool=clustalw&jobid=clustalw-20060323-21275762&poll=yes The immunogen of ab19348 was a bacterial fusion protein corresponding to the extracellular domain of N-cadherin (amino acids 160-724). The immunogen of ab167 was affinity purified 80kD extracellular fragments of E-Cadherin derived from tryptic digestion of A-431 human vulva carcinoma cells (amino acids 155-707). They are both monoclonals so it is difficult to predict what epitope may be recognized as this has not been mapped. The homology is only 46%, so it is likely they do not cross-react and there is no report that they do cross-react, but I cannot guarantee that they do not cross-react. Here is the alignment so you may make an educated guess: humanNcad MCRIAGALRTLLPLLLALLQASVEASGEIALCKTGFPEDVYSAVLS-KDVHEGQPLLNVK 59 ecad ----MGPWSRSLSALLLLLQVSSWLCQEPEPCHPGFDAESYTFTVPRRHLERGRVLGRVN 56 : : *. *. ** ***.* . * *:.** : *: .:. :.:..*: * .*: humanNcad FSNCNGKRKVQYESSEPADFKVDEDGMVYAVRSFPLSSEHAKFLIYAQDKETQEKWQVAV 119 ecad FEDCTGRQRTAYFSLD-TRFKVGTDGVITVKRPLRFHNPQIHFLVYAWDS-TYRKFSTKV 114 *.:*.*:::. * * : : ***. **:: . *.: : . : :**:** *. * .*:.. * humanNcad KLSLKPTLTEESVKESAEVEEIVFPRQFSKHSGHLQRQKRDWVIPPINLPENSRGPFPQE 179 ecad TLNTVGHHHRPPPHQASVSGIQAELLTFPNSSPGLRRQKRDWVIPPISCPENEKGPFPKN 174 .*. . . :::: . *.: * *:***********. ***.:****:: humanNcad LVRIRSDRDKNLSLRYSVTGPGADQPPTGIFIINPISGQLSVTKPLDREQIARFHLRAHA 239 ecad LVQIKSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHA 234 **:*:*::**: .: **:** *** **.*:***: :* *.**:*****:** : * :** humanNcad VDINGNQVENPIDIVINVIDMNDNRPEFLHQVWNGTVPEGSKPGTYVMTVTAIDADD-PN 298 ecad VSSNGNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSVMEVTATDADDDVN 294 *. *** **:*::*:*.* * ***:*** ::*::*:* **: *** ** *** **** * humanNcad ALNGMLRYRIVSQAPSTPSPNMFTINNETGDIITVAAGLDREKVQQYTLIIQATDMEGNP 358 ecad TYNAAIAYTILSQDPELPDKNMFTINRNTGVISVVTTGLDRESFPTYTLVVQAADLQGE- 353 : *. : * *:** *. *. ******.:** * .*::*****.. ***::**:*::*: humanNcad TYGLSNTATAVITVTDVNDNPPEFTAMTFYGEVPENRVDIIVANLTVTDKDQPHTPAWNA 418 ecad --GLSTTATAVITVTDTNDNPPIFNPTTYKGQVPENEANVVITTLKVTDADAPNTPAWEA 411 ***.**********.***** *.. *: *:****..:::::.*.*** * *:****:* humanNcad VYRISGGDPTGRFAIQTDPNSNDGLVTVVKPIDFETNRMFVLTVAAENQVPLAKGIQHPP 478 ecad VYTILNDD-GGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTT-- 468 ** * ..* *:*.: *:* .***::...* :***::: ::* **. * **: .: humanNcad QSTATVSVTVIDVNENPYFAPNPKIIRQEEGLHAGTMLTTFTAQDPDRYMQQNIRYTKLS 538 ecad -STATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFMEQKITYRIWR 527 *****:* *:**** * *.* * :. .*.: .* :*::***:** :*:*:* * humanNcad DPANWLKIDPVNGQITTIAVLDRE-SPNVKNNIYNATFLASDNGIPPMSGTGTLQIYLLD 597 ecad DTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVATGTGTLLLILSD 587 *.****:*:* .* *:* * **** :***. *.* ::*:*** * :***** : * * humanNcad INDNAPQVLPQEAETCETPDPNSINITALDYDIDPNAGPFAFDLPLSPVTIKRNWTITRL 657 ecad VNDNAPIPEPRTIFFCER-NPKPQVINIIDADLPPNTSPFTAELTHG---ASANWTIQYN 643 :***** *: ** :*:. *. :* *: **:.**: :*. . : . **** humanNcad NGDFAQLNLKIKF-LEAGIYEVPIIITDSGNPPKSNISILRVKVCQCDSNGDCTDVDRIV 716 ecad DPTQESIILKPKMALEVGDYKINLKLMDNQN--KDQVTTLEVSVCDCEGAAGVCRKAQPV 701 : .: ** *: **.* *:: : : *. * *.::: *.*.**:*:. .. : * humanNcad GAGLGTGAIIAILLCIIILLILVLMFVVWMKRRDKERQAKQLLIDPEDDVRDNILKYDEE 776 ecad EAGLQIPAILGILGGILALLILILLLLLFLRRR---AVVKEPLLPPEDDTRDNVYYYDEE 758 *** **:.** *: ****:*:::::::** .*: *: ****.***: **** humanNcad GGGEEDQDYDLSQLQQPDTVEPDAIKPVGIRRMDERPIHAEPQYPVRSAAPHPGDIGDFI 836 ecad GGGEEDQDFDLSQLHRGLDARPEVTR-----NDVAPTLMSVPRYLPRPANPD--EIGNFI 811 ********:*****:: ..*:. : : : . .: : *:* *.* *. :**:** humanNcad NEGLKAADNDPTAPPYDSLLVFDYEGSGSTAGSLSSLNSSSSGGEQDYDYLNDWGPRFKK 896 ecad DENLKAADTDPTAPPYDSLLVFDYEGSGSEAASLSSLNSSESDKDQDYDYLNEWGNRFKK 871 :*.*****.******************** *.********.*. :*******:** **** humanNcad LADMYGGGDD- 906 ecad LADMYGGGEDD 882 ********:* I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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