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Anti-Myosin Phosphatase (phospho T853)抗体(ab59203)

Reviews (1)Q&A (1)References (6)
Western blot - Anti-Myosin Phosphatase (phospho T853) antibody (ab59203)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Phosphatase (phospho T853) antibody (ab59203)

Key features and details

  • Rabbit polyclonal to Myosin Phosphatase (phospho T853)
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-Myosin Phosphatase (phospho T853)抗体
    参阅全部 Myosin Phosphatase 一抗

  • 描述

    兔多克隆抗体to Myosin Phosphatase (phospho T853)

  • 宿主

    Rabbit

  • 特异性

    Detects human Myosin Phosphatase only when phosphorylated at threonine 853 (threonine 852 for mouse and threonine 855 for rat)

  • 经测试应用

    适用于: WB, IHC-Pmore details

  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Rat

  • 免疫原

    Synthetic peptide corresponding to Human Myosin Phosphatase (phospho T853). (RSTPGV)

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • 存储溶液

    pH: 7
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

    Without Mg+2 and Ca+2
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • 纯化说明

    Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab59203于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 115 kDa).
IHC-P
Use at an assay dependent concentration.
说明
WB
1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 115 kDa).
IHC-P
Use at an assay dependent concentration.

靶标

  • 功能

    Regulates myosin phosphatase activity.

  • 组织特异性

    Expressed in striated muscles, specifically in type 2a fibers (at protein level).

  • 序列相似性

    Contains 6 ANK repeats.

  • 发展阶段

    Induced by 2-fold during pregnancy, including in abdominus rectus muscle.

  • 结构域

    Heterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase.

  • 翻译后修饰

    Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
    In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1.

  • 细胞定位

    Cytoplasm. Along actomyosin filaments and stress fibers.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • M130 antibody
    • MBS antibody
    • Myosin binding subunit antibody
    • Myosin phosphatase target subunit 1 antibody
    • Myosin phosphatase targeting subunit 1 antibody
    • Myosin phosphatase-targeting subunit 1 antibody
    • MYPT1 antibody
    • MYPT1_HUMAN antibody
    • PPP1R12A antibody
    • Protein phosphatase 1 regulatory inhibitor subunit 12A antibody
    • Protein phosphatase 1 regulatory subunit 12A antibody
    • Protein phosphatase 1, regulatory (inhibitor) subunit 12A antibody
    • Protein phosphatase myosin binding subunit antibody
    • Protein phosphatase myosin-binding subunit antibody
    see all

图片

  • Western blot - Anti-Myosin Phosphatase (phospho T853) antibody (ab59203)
    Western blot - Anti-Myosin Phosphatase (phospho T853) antibody (ab59203)
    All lanes : Anti-Myosin Phosphatase (phospho T853) antibody (ab59203) at 1/500 dilution

    Lane 1 : NIH/3T3 cell extract
    Lane 2 : NIH/3T3 cell extract with immunizing phosphopeptide

    Predicted band size: 115 kDa
    Observed band size: 130 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Phosphatase (phospho T853) antibody (ab59203)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Phosphatase (phospho T853) antibody (ab59203)
    Immunohistochemical analysis of paraffin embedded human colon carcinoma tissue using ab59203 at 1/50 dilution. Samples were treated -/+ phosphopeptide.

文献 (6)

发表研究结果有使用 ab59203？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab59203 被引用在 6 文献中.

  • Ma L  et al. Leukocyte Rho kinase activity and serum cystatin C affect cardiovascular events in acute coronary syndrome. Medicine (Baltimore) 99:e20060 (2020). PubMed: 32664054
  • Zhang M  et al. Protective effects of 18ß-glycyrrhetinic acid on pulmonary arterial hypertension via regulation of Rho A/Rho kinsase pathway. Chem Biol Interact 311:108749 (2019). PubMed: 31325423
  • Kao YH  et al. Cyclic Mechanical Stretch Up-regulates Hepatoma-Derived Growth Factor Expression in Cultured Rat Aortic Smooth Muscle Cells. Biosci Rep N/A:N/A (2018). WB ; Rat . PubMed: 29467272
  • Wang XQ  et al. Resveratrol enhances vascular reactivity in mice following lipopolysaccharide challenge via the RhoA-ROCK-MLCP pathway. Exp Ther Med 14:308-316 (2017). PubMed: 28672931
  • Cheng CI  et al. The prognostic values of leukocyte rho kinase activity in acute ischemic stroke. Biomed Res Int 2014:214587 (2014). WB ; Human . PubMed: 24716192
  • Montalvo J  et al. ROCK1 & 2 perform overlapping and unique roles in angiogenesis and angiosarcoma tumor progression. Curr Mol Med 13:205-19 (2013). WB . PubMed: 22934846

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-Myosin Phosphatase (phospho T853) antibody

 Poor
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Left ventricle)
Loading amount
20 µg
Specification
Left ventricle
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 29 2012

Question

ReviewID: xxx AbID: 59203, Anti-Myosin Phosphatase (phospho T853) antibody Rating: Poor Image: Anti-Myosin Phosphatase (phospho T853) antibody for Western blot (Mouse) Sample: Species: Mouse Type: Tissue lysate - whole Loading amount: 20 µg Specification: Left ventricle Application: Application: Gel Running Conditions: Reduced Denaturing (8%) Blocking step: BSA as blocking agent for 1 hour at 22°C Blocking Concentration: 5% Other detail: Dilution: 1/500 Incubation time: 2 hours at 22°C Diluent: 5% BSA in TBS-T Secondary Antibody: Name: Non-Abcam Antibody was used: Donkey anti-rabbit Conjugation: HRP Dilution: 1/15000 Detection: Detection method: ECL+ Exposure: 20 seconds Additional Data: Additional Notes: Tried a number of different things, incl. 5% MM in TBS-T, but nothing worked.

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Answer

Thank you for submitting an Abreview of ab59203. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in a tested species and application, we would like to follow up on this to see if we can possibly improve the results you are seeing with this antibody.

Based on the information in your review, I feel that altering the following step in your protocol may help improve your results:

- Have you tried detecting the total protein? If so, did you obtain the bands at the right MW?
- Are you certain the protein is phosphorylated under the experimental conditions used? And did you include phosphatase inhibitors when preparing the lysate? This would be crucial for detecting the phospho-site.
- Loading more protein (e.g. up to 50 ug) can help with the signal.
- Also, longer incubation time with the primary antibody (overnight, 4 degree) might be of help as well.
- As for the smears you see, this could be related to the secondary antibody (trying a no primary control might determine that) or to contaminated detection reagents (using fresh blocking and/or ECL solutions might help with that)

If you decide to follow these suggestions, please let me know if they are helpful.

Additionally, this antibody is covered by our Abpromise, so if the suggestions do not help and the antibody was purchased within the past 6 months, I would be happy to discuss a refund or replacement with you to resolve this issue.
Please let me know your order or PO number in this regard.

I look forward to your reply.

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