Anti-Myosin Phosphatase抗体(ab70809)
Key features and details
- Rabbit polyclonal to Myosin Phosphatase
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Myosin Phosphatase抗体
参阅全部 Myosin Phosphatase 一抗 -
描述
兔多克隆抗体to Myosin Phosphatase -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to a region between residue 800 and the C-terminus (residue 842) of human Myosin Phosphatase (NP_002471.1)
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阳性对照
- Whole cell lysate from HeLa or 293T cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70809于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
1/2000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 115 kDa).
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| IP | (1) |
Use at 2-5 µg/mg of lysate.
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| 说明 |
|---|
|
WB
1/2000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 115 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Regulates myosin phosphatase activity. -
组织特异性
Expressed in striated muscles, specifically in type 2a fibers (at protein level). -
序列相似性
Contains 6 ANK repeats. -
发展阶段
Induced by 2-fold during pregnancy, including in abdominus rectus muscle. -
结构域
Heterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase. -
翻译后修饰
Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1. -
细胞定位
Cytoplasm. Along actomyosin filaments and stress fibers. - Information by UniProt
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数据库链接
- Entrez Gene: 4659 Human
- Entrez Gene: 17931 Mouse
- Entrez Gene: 397190 Pig
- Entrez Gene: 116670 Rat
- Omim: 602021 Human
- SwissProt: O14974 Human
- SwissProt: Q9DBR7 Mouse
- SwissProt: Q10728 Rat
see all -
别名
- M130 antibody
- MBS antibody
- Myosin binding subunit antibody
see all
图片
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Western blot - Anti-Myosin Phosphatase antibody (ab70809)All lanes : Anti-Myosin Phosphatase antibody (ab70809) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 115 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds -
Immunoprecipitation - Anti-Myosin Phosphatase antibody (ab70809)1mg whole lysate from HeLa cells was immunoprecipitated using ab70809 at 3ug/mg of lysate (lane 1) or a control rabbit Ig (lane 2). For the subsequent western blot, 20% of the immunoprecipitate was loaded per lane, and probed with ab70809 at 1ug/ml. Detection: chemiluminescence with exposure time of 10 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab70809 被引用在 3 文献中.
- Betancor G et al. MX2-mediated innate immunity against HIV-1 is regulated by serine phosphorylation. Nat Microbiol 6:1031-1042 (2021). PubMed: 34282309
- Sastre E et al. Decompensated liver cirrhosis and neural regulation of mesenteric vascular tone in rats: role of sympathetic, nitrergic and sensory innervations. Sci Rep 6:31076 (2016). WB ; Rat . PubMed: 27484028
- Schein A et al. Identification of antisense long noncoding RNAs that function as SINEUPs in human cells. Sci Rep 6:33605 (2016). PubMed: 27646849