重组Anti-Myosin (MYH 1/2)抗体[A4.1025] (ab37484)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [A4.1025] to Myosin
- Suitable for: IHC-P, IHC-Fr, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Myosin (MYH 1/2)抗体[A4.1025]
参阅全部 Myosin 一抗 -
描述
小鼠单克隆抗体[A4.1025] to Myosin -
宿主
Mouse -
特异性
ATP binding loop of myosin heavy chain skeletal and cardiac muscle.
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经测试应用
适用于: IHC-P, IHC-Fr, ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Full length native protein (purified). This information is considered to be commercially sensitive.
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阳性对照
- WB: Human, mouse and rat skeletal muscle tissue lysates; Differentiated C2C12 whole cell lysate IHC-P: Human skeletal and cardiac muscle tissue. IHC-Fr: Mouse and rat skeletal muscle tissue. ICC/IF: Differentiated C2C12 cells.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
A4.1025 -
同种型
IgG2a -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab37484于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
Use a concentration of 0.807 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IHC-Fr |
Use a concentration of 16.14 µg/ml.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
|
ICC/IF |
Use a concentration of 3.228 µg/ml.
|
|
WB |
Use a concentration of 0.1614 - 0.807 µg/ml. Detects a band of approximately 223 kDa (predicted molecular weight: 223 kDa).
|
说明 |
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IHC-P
Use a concentration of 0.807 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
Use a concentration of 16.14 µg/ml. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
ICC/IF
Use a concentration of 3.228 µg/ml. |
WB
Use a concentration of 0.1614 - 0.807 µg/ml. Detects a band of approximately 223 kDa (predicted molecular weight: 223 kDa). |
靶标
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功能
Muscle contraction. -
序列相似性
Contains 1 IQ domain.
Contains 1 myosin head-like domain. -
结构域
The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2). -
细胞定位
Cytoplasm > myofibril. Thick filaments of the myofibrils. - Information by UniProt
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数据库链接
- Entrez Gene: 4619 Human
- Entrez Gene: 4620 Human
- Entrez Gene: 4621 Human
- Entrez Gene: 4622 Human
- Entrez Gene: 4624 Human
- Entrez Gene: 4632 Human
- Entrez Gene: 4633 Human
- Entrez Gene: 4634 Human
see all -
别名
- adult 1 antibody
- MYH1 antibody
- MYH1_HUMAN antibody
see all
图片
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Anti-Myosin (MYH 1/2) antibody [A4.1025] (ab37484) at 0.1614 µg/ml + Human skeletal muscle tissue lysate at 20 µg
Secondary
Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 223 kDa
Observed band size: 223 kDa
Exposure time: 3 secondsThe extra bands under 223KDa were mainly caused by degradation (PMID: 3295257).
Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin antibody [A4.1025] (ab37484)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Myosin (MYH1/2) with ab37484 at 0.807 µg/ml, followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on human skeletal muscle. The section was incubated with ab37484 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse skeletal muscle tissue labeling Myosin (MYH1/2) with ab37484 at 16.14 µg/ml followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse skeletal muscle is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C2C12 cells labelling Myosin (MYH1/2) with ab37484 at 3.228 µg/ml, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2µg/ml) (Green). Confocal image showing cytoplasmic staining in differentiated (treated) C2C12 cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (2.5µg/ml), followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (4µg/ml) (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2µg/ml).
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All lanes : Anti-Myosin (MYH 1/2) antibody [A4.1025] (ab37484) at 0.807 µg/ml
Lane 1 : Mouse skeletal muscle tissue lysate
Lane 2 : Rat skeletal muscle tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 223 kDa
Observed band size: 223 kDaExposure times: Lane 1: 3 secs; Lane 2: 1 sec.
Blocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Myosin (MYH 1/2) antibody [A4.1025] (ab37484) at 0.807 µg/ml
Lane 1 : C2C12 (mouse myoblasts) whole cell lysate
Lane 2 : Differentiated C2C12 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 223 kDa
Observed band size: 223 kDa
Exposure time: 3 secondsThe expression profile observed is consistent with what has been described in the literature (PMID: 26010876).
Blocking/Dilution buffer: 5% NFDM/TBST.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin antibody [A4.1025] (ab37484)
Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Myosin (MYH1/2) with ab37484 at 0.807 µg/ml, followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on human cardiac muscle. The section was incubated with ab37484 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin antibody [A4.1025] (ab37484)
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Myosin (MYH1/2) with ab37484 at 0.807 µg/ml, followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on mouse skeletal muscle. The section was incubated with ab37484 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin antibody [A4.1025] (ab37484)
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Myosin (MYH1/2) with ab37484 at 0.807 µg/ml, followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on rat skeletal muscle. The section was incubated with ab37484 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat skeletal muscle tissue labeling Myosin (MYH1/2) with ab37484 at 16.14 µg/ml followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat skeletal muscle is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab37484 被引用在 3 文献中.
- Li W et al. Construction of Tissue-Engineered Bladder Scaffolds with Composite Biomaterials. Polymers (Basel) 14:N/A (2022). PubMed: 35808700
- Liu Z et al. YAP Promotes Cell Proliferation and Stemness Maintenance of Porcine Muscle Stem Cells under High-Density Condition. Cells 10:N/A (2021). PubMed: 34831292
- Hughes SM & Blau HM Muscle fiber pattern is independent of cell lineage in postnatal rodent development. Cell 68:659-71 (1992). PubMed: 1531450