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Signal Transduction Cytoskeleton / ECM Cytoskeleton Motor Proteins Myosin

Anti-Myosin light chain (phospho S20)抗体(ab2480)

  • Datasheet
  • SDS
Reviews (17)Q&A (16)References (116)

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Western blot - Anti-Myosin light chain (phospho S20) antibody (ab2480)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain (phospho S20) antibody (ab2480)
  • Western blot - Anti-Myosin light chain (phospho S20) antibody (ab2480)

Key features and details

  • Rabbit polyclonal to Myosin light chain (phospho S20)
  • Suitable for: ELISA, ICC/IF, IHC-FoFr, WB, IHC-P, IP
  • Reacts with: Mouse, Rat, Human, Caenorhabditis elegans, Recombinant fragment
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Myosin light chain (phospho S20)抗体
    参阅全部 Myosin light chain 一抗
  • 描述

    兔多克隆抗体to Myosin light chain (phospho S20)
  • 宿主

    Rabbit
  • 特异性

    This phospho specific polyclonal antibody is specific for phosphorylated pS20 of human myosin light chain. Reactivity with non-phosphorylated human myosin light chain is less than 1% by ELISA. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences. Cross reactivity is expected.

    Customers have seen variable performance with Xenopus laevis.

  • 经测试应用

    适用于: ELISA, ICC/IF, IHC-FoFr, WB, IHC-P, IPmore details
  • 种属反应性

    与反应: Mouse, Rat, Human, Caenorhabditis elegans, Recombinant fragment
    预测可用于: Bird, Mammals
  • 免疫原

    Synthetic peptide corresponding to Human Myosin light chain aa 1-100 (phospho S20) conjugated to keyhole limpet haemocyanin.
    Database link: J3QRS3

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    This antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Motor Proteins
    • Myosin
    • Stem Cells
    • Mesenchymal Stem Cells
    • Myogenesis
    • Cardiovascular
    • Heart
    • Contractility
    • Contractile Proteins
    • Myosins

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2480于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
Use at an assay dependent concentration.
ICC/IF (5)
Use at an assay dependent concentration.
IHC-FoFr
Use at an assay dependent concentration. PubMed: 19279134

Trichloroacetic acid was used for tissue fixation.

WB (7)
1/500 - 1/2000. Predicted molecular weight: 20 kDa.

We recommend using BSA for blocking when using phospho-specific antibodies.

IHC-P (4)
Use a concentration of 2.5 µg/ml.
IP
Use at an assay dependent concentration.
说明
ELISA
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-FoFr
Use at an assay dependent concentration. PubMed: 19279134

Trichloroacetic acid was used for tissue fixation.

WB
1/500 - 1/2000. Predicted molecular weight: 20 kDa.

We recommend using BSA for blocking when using phospho-specific antibodies.

IHC-P
Use a concentration of 2.5 µg/ml.
IP
Use at an assay dependent concentration.

靶标

  • 功能

    Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
  • 组织特异性

    Smooth muscle tissues and in some, but not all, nonmuscle cells.
  • 序列相似性

    Contains 3 EF-hand domains.
  • 翻译后修饰

    Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.
  • Target information above from: UniProt accession P24844 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 10398 Human
    • Entrez Gene: 98932 Mouse
    • Entrez Gene: 296313 Rat
    • Omim: 609905 Human
    • SwissProt: P24844 Human
    • SwissProt: Q9CQ19 Mouse
    • SwissProt: Q64122 Rat
    • Unigene: 504687 Human
    • Unigene: 271770 Mouse
    • Unigene: 228729 Rat
    • Unigene: 6870 Rat
    see all
  • 别名

    • 20 kDa myosin light chain antibody
    • KMLC antibody
    • LC20 antibody
    • MLC-2C antibody
    • MLCK2 antibody
    • MRLC1 antibody
    • MYL9 antibody
    • MYL9_HUMAN antibody
    • Myosin regulatory light chain 2 antibody
    • Myosin regulatory light chain 2, smooth muscle isoform antibody
    • Myosin regulatory light chain 9 antibody
    • Myosin regulatory light chain MRLC1 antibody
    • Myosin regulatory light polypeptide 9 antibody
    • Myosin RLC antibody
    • Myosin, light chain 9, regulatory antibody
    • MYRL2 antibody
    • smooth muscle isoform antibody
    see all

图片

  • Western blot - Anti-Myosin light chain (phospho S20) antibody (ab2480)
    Western blot - Anti-Myosin light chain (phospho S20) antibody (ab2480)
    All lanes : Anti-Myosin light chain (phospho S20) antibody (ab2480) at 1/1000 dilution

    Lane 1 : Regulatory Light Chain Non-Phospho recombinant protein, 50 ng
    Lane 2 : Regulatory Light Chain Phospho recombinant protein, 50 ng
    Lane 3 : Smooth Muscle Non-Phospho recombinant protein, 50 ng
    Lane 4 : Smooth Muscle Phospho recombinant protein, 50 ng

    Secondary
    All lanes : Peroxidase rabbit secondary antibody at 1/40000 dilution

    Predicted band size: 20 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain (phospho S20) antibody (ab2480)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain (phospho S20) antibody (ab2480)

    IHC-P of ab2480 at 2.5 µg/ml staining both vascular and myometrial smooth muscle cells of the human uterus. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

  • Western blot - Anti-Myosin light chain (phospho S20) antibody (ab2480)
    Western blot - Anti-Myosin light chain (phospho S20) antibody (ab2480)

    Rabbit polyclonal to phospho Myosin Light Chain phospho Myosin Light Chain (Ser 20) (ab2480) used at a 1/5000 dilution to detect myosin light chain by Western blot. Either 13 ug (lanes 1-3) or 20 ug (lanes 4-7) of a mouse cardiac myocyte lysate was loaded on a 4-20% Criterion gel for SDS-PAGE.  Samples were either mock-treated or CLA treated:
    Lane 1 : untreated 45 min
    Lane 2 : CLA 50 nm 45 min
    Lane 3 : CLA 100 nm 45 min
    Lane 4 : A21 untreated 45 min
    Lane 5 : A22 A23187 5 min
    Lane 6 : A23 A23187 15 min
    Lane 7 : A24 A23187 60 min
    After washing, a 1/5,000 dilution of anti-rabbit HRP (ab7090) was used as secondary.

    Rabbit polyclonal to phospho Myosin Light Chain phospho Myosin Light Chain (Ser 20) (ab2480) used at a 1/5000 dilution to detect myosin light chain by Western blot. Either 13 ug (lanes 1-3) or 20 ug (lanes 4-7) of a mouse cardiac myocyte lysate was loaded on a 4-20

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (116)

发表研究结果有使用 ab2480?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab2480 被引用在 116 文献中.

  • Li B  et al. Iron deficiency promotes aortic medial degeneration via destructing cytoskeleton of vascular smooth muscle cells. Clin Transl Med 11:e276 (2021). PubMed: 33463069
  • Winter MR  et al. Calcium-vesicles perform active diffusion in the sea urchin embryo during larval biomineralization. PLoS Comput Biol 17:e1008780 (2021). PubMed: 33617532
  • Gong W  et al. CCL4-mediated targeting of spleen tyrosine kinase (Syk) inhibitor using nanoparticles alleviates inflammatory bowel disease. Clin Transl Med 11:e339 (2021). PubMed: 33634985
  • Hatte G  et al. Adherens junctions are involved in polarized contractile ring formation in dividing epithelial cells of Xenopus laevis embryos. Exp Cell Res 402:112525 (2021). PubMed: 33662366
  • Young RE  et al. Smooth Muscle Differentiation Is Essential for Airway Size, Tracheal Cartilage Segmentation, but Dispensable for Epithelial Branching. Dev Cell 53:73-85.e5 (2020). PubMed: 32142630
View all Publications for this product

客户评价及客户问答

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1-10 of 33 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Myosin light chain (phospho S20) antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Intestine)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Sodium Citrate pH6
Permeabilization
No
Specification
Intestine
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Frazer Bell

Verified customer

提交于 Feb 14 2023

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Myosin light chain (phospho S20) antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cat Tissue sections (Ileum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH6
Permeabilization
No
Specification
Ileum
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Frazer Bell

Verified customer

提交于 Feb 14 2023

Immunocytochemistry/ Immunofluorescence abreview for Anti-Myosin light chain (phospho S20) antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U87-MG)
Permeabilization
Yes - 0.5% triton
Specification
U87-MG
Blocking step
Goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Dec 02 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-Myosin light chain (phospho S20) antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (MDCK)
Permeabilization
Yes - 0.2% Triton X100
Specification
MDCK
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 11 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Myosin light chain (phospho S20) antibody

Good
Abreviews
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (head)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA based buffer pH 7.8
Permeabilization
No
Specification
head
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Sep 13 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Myosin light chain (phospho S20) antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Prostate)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate, pH 6
Permeabilization
No
Specification
Prostate
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 28 2017

IHC - Wholemount abreview for Anti-Myosin light chain (phospho S20) antibody

Excellent
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Hydra vulgaris Tissue (morphogenesis)
Specification
morphogenesis
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Oliver Holz

Verified customer

提交于 Jul 21 2017

Western blot abreview for Anti-Myosin light chain (phospho S20) antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions
Reduced Denaturing (4-12% BIS TRIS)
Loading amount
15 µg
Specification
Intestinal tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Sep 08 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-Myosin light chain (phospho S20) antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human arterial myocytes)
Specification
Human arterial myocytes
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 08 2015

Western blot

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (15)
Sample
Human Tissue lysate - whole (Heart)
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 May 22 2013

1-10 of 33 Abreviews or Q&A

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