重组Anti-Myosin light chain 3抗体[EPR4160] (ab108923)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)](https://www.abcam.cn/ps/products/108/ab108923/Images/ab108923-121404-anti-myosin-light-chain-3-antibody-epr4160-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4160] to Myosin light chain 3
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Myosin light chain 3抗体[EPR4160]
参阅全部 Myosin light chain 3 一抗 -
描述
兔单克隆抗体[EPR4160] to Myosin light chain 3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- human heart muscle lysate, human skeletal muscle lysate; human cardiac muscle tissue, human skeletal muscle tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20ºC. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4160 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108923于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 22 kDa.
|
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 22 kDa. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Regulatory light chain of myosin. Does not bind calcium. -
疾病相关
Defects in MYL3 are the cause of cardiomyopathy familial hypertrophic type 8 (CMH8) [MIM:608751]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. CMH8 inheritance can be autosomal dominant or recessive.
Defects in MYL3 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 1 (MVC1) [MIM:608751]. MVC1 is a very rare variant of familial hypertrophic cardiomyopathy, characterized by mid-left ventricular chamber thickening. -
序列相似性
Contains 3 EF-hand domains. -
翻译后修饰
The N-terminus is blocked.
N-terminus is methylated by METTL11A/NTM1. - Information by UniProt
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数据库链接
- Entrez Gene: 4634 Human
- Entrez Gene: 17897 Mouse
- Entrez Gene: 24585 Rat
- Omim: 160790 Human
- SwissProt: P08590 Human
- SwissProt: P09542 Mouse
- SwissProt: P16409 Rat
- Unigene: 517939 Human
see all -
别名
- Cardiac myosin light chain 1 antibody
- CMH8 antibody
- CMLC1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)
Immunohistochemical staining of paraffin-embedded human cardiac muscle using ab108923 at a dilution of 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)](https://www.abcam.cn/ps/products/108/ab108923/Images/ab108923-121403-anti-myosin-light-chain-3-antibody-epr4160-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)Immunohistochemical staining of paraffin-embedded human skeletal muscle tissue using ab108923 at a diltion of 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)](https://www.abcam.cn/ps/products/108/ab108923/Images/ab108923-121402-anti-myosin-light-chain-3-antibody-epr4160-western-blot.jpg)
Western blot - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)All lanes : Anti-Myosin light chain 3 antibody [EPR4160] (ab108923) at 1/1000 dilution
Lane 1 : human heart muscle tissue lysate
Lane 2 : human skeletal muscle tissue lysate
Lysates/proteins at 10 µg/ml per lane.
Predicted band size: 22 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)](https://www.abcam.cn/ps/products/108/ab108923/Images/ab108923-121401-anti-myosin-light-chain-3-antibody-epr4160-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)ab108923 showing negative staining in Normal brain tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)](https://www.abcam.cn/ps/products/108/ab108923/Images/ab108923-121400-anti-myosin-light-chain-3-antibody-epr4160-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)ab108923 showing negative staining in Normal liver tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)](https://www.abcam.cn/ps/products/108/ab108923/Images/ab108923-121399-anti-myosin-light-chain-3-antibody-epr4160-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)ab108923 showing negative staining in Normal tonsil tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)](https://www.abcam.cn/ps/products/108/ab108923/Images/ab108923-121398-anti-myosin-light-chain-3-antibody-epr4160-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)ab108923 showing negative staining in Normal breast tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)](https://www.abcam.cn/ps/products/108/ab108923/Images/ab108923-8-benefits-of-recombinant-antibodies.png)
Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab108923 被引用在 2 文献中.
- Turco MP et al. Periurethral muscle-derived mononuclear cell injection improves urethral sphincter restoration in rats. Neurourol Urodyn 36:2011-2018 (2017). PubMed: 28346707
- Ren Y et al. Potential of adipose-derived mesenchymal stem cells and skeletal muscle-derived satellite cells for somatic cell nuclear transfer mediated transgenesis in Arbas Cashmere goats. PLoS One 9:e93583 (2014). ICC ; Goat . PubMed: 24699686