Anti-Myosin Light Chain 2抗体(ab79935)
Key features and details
- Rabbit polyclonal to Myosin Light Chain 2
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Myosin Light Chain 2抗体
参阅全部 Myosin Light Chain 2 一抗 -
描述
兔多克隆抗体to Myosin Light Chain 2 -
宿主
Rabbit -
特异性
From September 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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经测试应用
适用于: WB, IHC-P, IPmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Sheep, Cow, Dog, Pig
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免疫原
Synthetic peptide corresponding to Human Myosin Light Chain 2 aa 100 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab88166) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab79935于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
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| IHC-P |
Use a concentration of 5 µg/ml.
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| IP |
Use a concentration of 5 µg/ml.
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| 说明 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa). |
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IHC-P
Use a concentration of 5 µg/ml. |
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IP
Use a concentration of 5 µg/ml. |
靶标
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疾病相关
Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10) [MIM:608758]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2) [MIM:608758]. MVC2 is a very rare variant of familial hypertrophic cardiomyopathy, characterized by mid-left ventricular chamber thickening. -
序列相似性
Contains 3 EF-hand domains. -
翻译后修饰
N-terminus is methylated by METTL11A/NTM1. - Information by UniProt
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数据库链接
- Entrez Gene: 505519 Cow
- Entrez Gene: 403614 Dog
- Entrez Gene: 4633 Human
- Entrez Gene: 17906 Mouse
- Entrez Gene: 363925 Rat
- Omim: 160781 Human
- SwissProt: Q3SZE5 Cow
- SwissProt: P10916 Human
see all -
别名
- Cardiac myosin light chain-2 antibody
- Cardiac ventricular myosin light chain 2 antibody
- CMH10 antibody
see all
图片
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Immunoprecipitation - Anti-Myosin Light Chain 2 antibody (ab79935)
Myosin Light Chain 2 was immunoprecipitated using 0.5mg Mouse Heart tissue, 5µg of Rabbit polyclonal to Myosin Light Chain 2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Heart tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab79935.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 19kDa; Myosin Light Chain 2
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody (ab79935)IHC image of Myosin light chain 2 antibody staining in a section of formalin-fixed paraffin-embedded normal mouse heart performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab79935, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Western blot - Anti-Myosin Light Chain 2 antibody (ab79935)All lanes : Anti-Myosin Light Chain 2 antibody (ab79935) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 3 : Heart (Mouse) Tissue Lysate
Lane 4 : Heart (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 19 kDa
Observed band size: 19 kDa
Additional bands at: 17 kDa, 8 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 15 minutes
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (34)
ab79935 被引用在 34 文献中.
- Chapotte-Baldacci CA et al. Biophysical properties of NaV1.5 channels from atrial-like and ventricular-like cardiomyocytes derived from human induced pluripotent stem cells. Sci Rep 13:20685 (2023). PubMed: 38001331
- Bornstein B et al. Molecular characterization of the intact mouse muscle spindle using a multi-omics approach. Elife 12:N/A (2023). PubMed: 36744866
- Yap L et al. Pluripotent stem cell-derived committed cardiac progenitors remuscularize damaged ischemic hearts and improve their function in pigs. NPJ Regen Med 8:26 (2023). PubMed: 37236990
- Wu X et al. Cardiac Development in the Presence of Cadmium: An in Vitro Study Using Human Embryonic Stem Cells and Cardiac Organoids. Environ Health Perspect 130:117002 (2022). PubMed: 36321828
- Gu Z et al. A Uterus-Inspired Niche Drives Blastocyst Development to the Early Organogenesis. Adv Sci (Weinh) 9:e2202282 (2022). PubMed: 35843885