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Signal Transduction Cytoskeleton / ECM Cytoskeleton Motor Proteins Myosin

Anti-Myosin抗体[414] (ab8070)

  • Datasheet
  • SDS
Submit a review Q&A (5)

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Key features and details

  • Mouse monoclonal [414] to Myosin
  • Suitable for: IHC-Fr, ICC, IHC-P, WB
  • Reacts with: Chicken
  • Isotype: IgM

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概述

  • 产品名称

    Anti-Myosin抗体[414]
    参阅全部 Myosin 一抗
  • 描述

    小鼠单克隆抗体[414] to Myosin
  • 宿主

    Mouse
  • 特异性

    This monoclonal antibody reacts in immunoblots specific with myosin and is specific to the 75kD of Myosin. Does not react with alpha-cardiac myosin. Does not react with the light-chain but with the last 75 kD of the myosin molecule. These 75 kD are located on a myosin-common site-chain.
  • 经测试应用

    适用于: IHC-Fr, ICC, IHC-P, WBmore details
  • 种属反应性

    与反应: Chicken
  • 阳性对照

    • Chicken muscle myomyofibrils.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液

    pH: 7.3
    Preservative: 0.1% Sodium azide
    Constituent: 1% BSA
  • Concentration information loading...
  • 纯度

    Tissue culture supernatant
  • Primary antibody说明

    This antibody, directed against myosin can be used for detection of myosin in frozen sections as well as in cultured cells. Applicable in paraffin sections only with microwave oven pretreatment. Also works in immunoblotting.
  • 克隆

    单克隆
  • 克隆编号

    414
  • 同种型

    IgM
  • 轻链类型

    unknown
  • 研究领域

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Motor Proteins
    • Myosin

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgM H&L (ab9167)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab8070于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-Fr
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.

Use at 1/5 - 1/10.(Only with microwave oven pretreatment. Based on the Indirect method, with a normal ABC method the dilution can be 5-10 times higher). Dilute immediately before use with PBS or Tris.

WB
Use at an assay dependent concentration.
说明
IHC-Fr
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.

Use at 1/5 - 1/10.(Only with microwave oven pretreatment. Based on the Indirect method, with a normal ABC method the dilution can be 5-10 times higher). Dilute immediately before use with PBS or Tris.

WB
Use at an assay dependent concentration.

靶标

  • 功能

    Muscle contraction.
  • 序列相似性

    Contains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • 结构域

    The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
  • 细胞定位

    Cytoplasm > myofibril. Thick filaments of the myofibrils.
  • Target information above from: UniProt accession P12882 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 别名

    • adult 1 antibody
    • MYH1 antibody
    • MYH1_HUMAN antibody
    • MYH2 antibody
    • MYH3 antibody
    • MYH4 antibody
    • MYH6 antibody
    • MyHC-2x antibody
    • MyHC-IIx/d antibody
    • MYL1 antibody
    • MYL2 antibody
    • MYL3 antibody
    • MYL5 antibody
    • MYL6 antibody
    • Myosin light chain 2 regulatory cardiac slow antibody
    • Myosin 1 antibody
    • Myosin heavy chain 1 antibody
    • Myosin heavy chain 1 skeletal muscle adult antibody
    • Myosin heavy chain 2 skeletal muscle adult antibody
    • Myosin heavy chain 2x antibody
    • Myosin heavy chain 3 skeletal muscle adult antibody
    • Myosin heavy chain 4 skeletal muscle adult antibody
    • Myosin heavy chain 6 skeletal muscle adult antibody
    • Myosin heavy chain antibody
    • Myosin heavy chain IIx/d antibody
    • Myosin heavy chain skeletal muscle adult 1 antibody
    • Myosin II antibody
    • Myosin light chain 1 alkali skeletal fast antibody
    • Myosin light chain 3 alkali ventricular skeletal slow antibody
    • Myosin light chain 4 alkali atrial embryonic antibody
    • Myosin light chain 5 regulatory antibody
    • Myosin light chain 6 alkali smooth muscle and non muscle antibody
    • Myosin-1 antibody
    • Myosin1 antibody
    • skeletal muscle antibody
    see all

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (0)

发表研究结果有使用 ab8070?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab8070 尚未被引用在任何文献中。

客户评价及客户问答

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1-5 of 5 Abreviews or Q&A

Question

Unfortunately I do not need another antibody, and I am going out on a limb by considering placing an order of over $1000 with a company we have never used before. If these antibodies work well for us, we will continue to buy from Abcam. However, considering the size of the order I am hoping you might be able to at least offer us free shipping (in line with other companies such as [competitor])? If you are still not able to help, I am required by my PI to reduce my order to below $1000 which means dropping one of the primary antibodies or looking to other sources for the order. Is there any way you might be able to make an exception this time, given that we will become regular customers should these antibodies work well for us? Failing this, are you able to offer trial sizes of your antibodies?

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Abcam community

Verified customer

Asked on May 02 2005

Answer

Thank you for responding so promptly. I'm sorry to hear you're not happy with my offer. At Abcam we value each and every one of our customers. However, I must stand by our policy. We do not routinely offer free or trial sized samples for testing purposes. If an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will award you 50 points with the Abcam Loyalty Scheme which can be redeemed on a number of rewards.

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Abcam Scientific Support

回复于 May 02 2005

Question

Hello, I am writing in order to find out if the antibody specified above is specific to smooth muscle since, in the datasheet, it stated that it does not react with alpha-cardiac myosin.

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Abcam community

Verified customer

Asked on Aug 08 2003

Answer

Thank you for your inquiry. Yes, the antibody can be used for smoothe muscle.

Read More

Abcam Scientific Support

回复于 Aug 22 2003

Question

I'd be happy to try a sample of the antibody. Depending upon when it arrives, I may need to order more of the antibody from you to get started on my experiments, regardless. Thanks,

Read More

Abcam community

Verified customer

Asked on Dec 17 2002

Answer

Thanks you for your message. The deadline for placing orders to be dispatched before the holidays is midday December 18th. Please do let me know how soon you need the products. Would it be all right at the beginning of January? Or is it too late? Please have a look at the Abcam homepage (top panel).

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Abcam Scientific Support

回复于 Dec 17 2002

Question

I just need an anti myocin antibody which can react with rat or mouse heart tissue. The sections are prepared as parafin embeded sections for immunohistochemistry technique. Thank you

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Abcam community

Verified customer

Asked on Nov 27 2001

Answer

This antibody will react with muscle and heart tissue, however it will not react with alpha-cardiac myosin. Furthermore this antibody has only been tested on human tissue and we suspect that it will not cross-react with mouse and/or rat although this has not actually been tested.

Read More

XXXXX XXXXX

Abcam Scientific Support

回复于 Nov 28 2001

Question

I am interested in antibodies for rat myosin heavy chains. This would include antibodies for embryonic, neonatal, type I, and type IIs (ie IIa, IIx, & IIb). I would like to use these for immuno histology on fresh frozen tissue section of rat skeletal muscle. Can you send me a list of catalogue numbers for any or all of the above? Thank you for your consideration.

Read More

Abcam community

Verified customer

Asked on Nov 08 2001

Answer

All the information we have for antibodies to myosin, (ab7784, ab7829, ab7786, ab7785,) is in the datasheets

Read More

Abcam Scientific Support

回复于 Nov 09 2001

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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