Anti-Myosin抗体[414] (ab8070)
Key features and details
- Mouse monoclonal [414] to Myosin
- Suitable for: IHC-Fr, ICC, IHC-P, WB
- Reacts with: Chicken
- Isotype: IgM
概述
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产品名称
Anti-Myosin抗体[414]
参阅全部 Myosin 一抗 -
描述
小鼠单克隆抗体[414] to Myosin -
宿主
Mouse -
特异性
This monoclonal antibody reacts in immunoblots specific with myosin and is specific to the 75kD of Myosin. Does not react with alpha-cardiac myosin. Does not react with the light-chain but with the last 75 kD of the myosin molecule. These 75 kD are located on a myosin-common site-chain. -
经测试应用
适用于: IHC-Fr, ICC, IHC-P, WBmore details -
种属反应性
与反应: Chicken -
阳性对照
- Chicken muscle myomyofibrils.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.3
Preservative: 0.1% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
纯度
Tissue culture supernatant -
Primary antibody说明
This antibody, directed against myosin can be used for detection of myosin in frozen sections as well as in cultured cells. Applicable in paraffin sections only with microwave oven pretreatment. Also works in immunoblotting. -
克隆
单克隆 -
克隆编号
414 -
同种型
IgM -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
|
|
| ICC |
Use at an assay dependent concentration.
|
|
| IHC-P |
Use at an assay dependent concentration.
Use at 1/5 - 1/10.(Only with microwave oven pretreatment. Based on the Indirect method, with a normal ABC method the dilution can be 5-10 times higher). Dilute immediately before use with PBS or Tris. |
|
| WB |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
|
ICC
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. Use at 1/5 - 1/10.(Only with microwave oven pretreatment. Based on the Indirect method, with a normal ABC method the dilution can be 5-10 times higher). Dilute immediately before use with PBS or Tris. |
|
WB
Use at an assay dependent concentration. |
靶标
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功能
Muscle contraction. -
序列相似性
Contains 1 IQ domain.
Contains 1 myosin head-like domain. -
结构域
The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2). -
细胞定位
Cytoplasm > myofibril. Thick filaments of the myofibrils. - Information by UniProt
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别名
- adult 1 antibody
- MYH1 antibody
- MYH1_HUMAN antibody
see all
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab8070 尚未被引用在任何文献中。