AB251338

重组Anti-MYOM1抗体[EPR17322] - BSA and Azide free

Anti-MYOM1 antibody [EPR17322] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal MYOM1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

查看别名

Myomesin-1, 190 kDa connectin-associated protein, 190 kDa titin-associated protein, Myomesin family member 1, MYOM1

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (AB251338)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (AB251338)

This data was developed using ab201228, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MYOM1 with ab201228 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Human tonsil tissue is a negative control for MYOM1. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (AB251338)

This data was developed using ab201228, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling MYOM1 with ab201228 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on Human skeletal muscle tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (AB251338)

This data was developed using ab201228, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling MYOM1 with ab201228 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on mouse skeletal muscle tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (AB251338)

This data was developed using ab201228, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling MYOM1 with ab201228 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on rat skeletal muscle tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (AB251338)

This data was developed using ab201228, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-MYOM1 antibody [EPR17322] (<a href='/products/primary-antibodies/myom1-antibody-epr17322-ab201228'>ab201228</a>) at 1/20000 dilution

All lanes:

Human fetal heart tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 188 kDa

Observed band size: 188 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (AB251338)

This data was developed using ab201228, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-MYOM1 antibody [EPR17322] (<a href='/products/primary-antibodies/myom1-antibody-epr17322-ab201228'>ab201228</a>) at 1/20000 dilution

Lane 1:

Mouse heart lysate at 20 µg

Lane 2:

Mouse muscle lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 188 kDa

Observed band size: 188 kDa

false

Exposure time: 1min

Supplier Data

Western blot - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (AB251338)

This data was developed using ab201228, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-MYOM1 antibody [EPR17322] (<a href='/products/primary-antibodies/myom1-antibody-epr17322-ab201228'>ab201228</a>) at 1/20000 dilution

Lane 1:

Rat heart lysate at 20 µg

Lane 2:

Rat brain lysate at 20 µg

Lane 3:

Rat kidney lysate at 20 µg

Lane 4:

C6 (Rat glial tumor cells) whole cell lysate at 20 µg

Lane 5:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 188 kDa

Observed band size: 188 kDa

false

Exposure time: 15s

不同偶联物与剂型 (1)

Unconjugated

Anti-MYOM1 antibody [EPR17322]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR17322

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse, Rat, Human

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

ab251338 is the carrier-free version of ab201228.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MYOM1 also known as myomesin 1 is a protein with a molecular mass of approximately 185 kDa. It plays a mechanical role in muscle cells where it contributes to the M-band region of the sarcomere. MYOM1 is important in the structural integrity and elasticity of the myofibrils. Expression of this protein is specifically seen in cardiac and skeletal muscle tissues indicating its significance in muscle function.

Biological function summary

The function of MYOM1 involves its contribution to sarcomere organization and stability. It acts as a scaffold in the M-band where it forms complexes with other sarcomeric proteins. These complexes include titin and muscle-specific kinase 1 which help maintain sarcomere structure during muscle contraction and stretch. MYOM1's interaction with these proteins is essential for muscle elasticity and proper muscle function.

Pathways

MYOM1 operates within the muscle contraction pathway and the sarcomere organization pathway. It interacts closely with titin which is a giant protein essential for muscle elasticity and with beta-catenin a protein involved in cell signaling and cytoskeleton organization. The interaction and the pathways help maintain muscle function and structural integrity ensuring proper muscle responses to physiological demands.

MYOM1 is linked to the development of myopathies particularly myosin storage myopathy and hypertrophic cardiomyopathy. These conditions are associated with mutations or malfunctions in proteins such as titin and myosin which are part of MYOM1's network. Understanding the connection between MYOM1 and these proteins helps in unraveling the pathology of these muscle disorders presenting potential targets for therapeutic interventions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Major component of the vertebrate myofibrillar M band. Binds myosin, titin, and light meromyosin. This binding is dose dependent.

See full target information MYOM1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 22:800 PubMed39731111

2024

Role of PCBP2 in regulating nanovesicles loaded with curcumin to mitigate neuroferroptosis in neural damage caused by heat stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Guo,Yizhan Wu,Guangjun Wang,Jiangwei Liu

View all publications

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重组Anti-MYOM1抗体[EPR17322] - BSA and Azide free