重组Anti-MYOM1抗体[EPR17322-9] (ab205618)
![Western blot - Anti-MYOM1 antibody [EPR17322-9] (ab205618)](https://www.abcam.cn/ps/products/205/ab205618/Images/ab205618-251801-ab205618WBa.jpg "Western blot - Anti-MYOM1 antibody [EPR17322-9] (ab205618)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17322-9] to MYOM1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
-
产品名称
Anti-MYOM1抗体[EPR17322-9]
参阅全部 MYOM1 一抗 -
描述
兔单克隆抗体[EPR17322-9] to MYOM1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB; Human fetal heart lysate; mouse heart and muscle lysates; rat heart lysate. IHC-P; Human cardiac muscle, mouse cardiac muscle and rat skeletal muscle tissues.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR17322-9 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab205618于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/5000. Detects a band of approximately 188 kDa (predicted molecular weight: 188 kDa).
|
|
| IHC-P |
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/5000. Detects a band of approximately 188 kDa (predicted molecular weight: 188 kDa). |
|
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
-
功能
Major component of the vertebrate myofibrillar M band. Binds myosin, titin, and light meromyosin. This binding is dose dependent. -
序列相似性
Contains 5 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. - Information by UniProt
-
数据库链接
- Entrez Gene: 8736 Human
- Entrez Gene: 17929 Mouse
- Entrez Gene: 316740 Rat
- Omim: 603508 Human
- SwissProt: P52179 Human
- SwissProt: Q62234 Mouse
- Unigene: 464469 Human
-
别名
- 190 kDa connectin-associated protein antibody
- 190 kDa titin-associated protein antibody
- EH myomesin antibody
see all
图片
-
Western blot - Anti-MYOM1 antibody [EPR17322-9] (ab205618)Anti-MYOM1 antibody [EPR17322-9] (ab205618) at 1/5000 dilution + Human fetal heart lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 188 kDa
Observed band size: 188 kDa
Exposure time: 2 minutesBlocking/dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-MYOM1 antibody [EPR17322-9] (ab205618)](https://www.abcam.cn/ps/products/205/ab205618/Images/ab205618-251802-ab205618WBb.jpg)
Western blot - Anti-MYOM1 antibody [EPR17322-9] (ab205618)All lanes : Anti-MYOM1 antibody [EPR17322-9] (ab205618) at 1/5000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse muscle lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 188 kDa
Observed band size: 188 kDa
Exposure time: 5 secondsBlocking/dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-MYOM1 antibody [EPR17322-9] (ab205618)](https://www.abcam.cn/ps/products/205/ab205618/Images/ab205618-251803-ab205618WBc.jpg)
Western blot - Anti-MYOM1 antibody [EPR17322-9] (ab205618)All lanes : Anti-MYOM1 antibody [EPR17322-9] (ab205618) at 1/5000 dilution
Lane 1 : Rat heart tissue lysate
Lane 2 : Rat brain tissue lysate
Lane 3 : Rat kidney tissue lysate
Lane 4 : C6 (Rat glial tumor cells) whole cell lysate
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/100000 dilution
Predicted band size: 188 kDa
Observed band size: 188 kDa
Exposure time: 5 secondsBlocking/dilution buffer: 5% NFDM/TBST.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322-9] (ab205618)](https://www.abcam.cn/ps/products/205/ab205618/Images/ab205618-251798-ab205618IHCa.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322-9] (ab205618)Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling MYOM1 with ab205618 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human cardiac muscle tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322-9] (ab205618)](https://www.abcam.cn/ps/products/205/ab205618/Images/ab205618-251797-ab205618IHCb.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322-9] (ab205618)Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling MYOM1 with ab205618 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. No staining on human tonsil tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322-9] (ab205618)](https://www.abcam.cn/ps/products/205/ab205618/Images/ab205618-251795-ab205618IHCc.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322-9] (ab205618)Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling MYOM1 with ab205618 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on mouse cardiac muscle tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322-9] (ab205618)](https://www.abcam.cn/ps/products/205/ab205618/Images/ab205618-251794-ab205618IHCd.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322-9] (ab205618)Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling MYOM1 with ab205618 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on rat skeletal muscle tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-MYOM1 antibody [EPR17322-9] (ab205618)](https://www.abcam.cn/ps/products/205/ab205618/Images/ab205618-9-benefits-of-recombinant-antibodies.png)
Anti-MYOM1 antibody [EPR17322-9] (ab205618)
数据表及文件
-
SDS download
-
Datasheet download
Certificate of Compliance
文献 (1)
ab205618 被引用在 1 文献中.
- Lynch JM et al. Mutant myocilin impacts sarcomere ultrastructure in mouse gastrocnemius muscle. PLoS One 13:e0206801 (2018). PubMed: 30395621