Anti-Myoferlin抗体[7D2] (ab76746)
![Immunocytochemistry/ Immunofluorescence - Anti-Myoferlin antibody [7D2] (ab76746)](https://www.abcam.cn/ps/products/76/ab76746/Images/ab76746-92289-anti-myoferlin-antibody-7d2-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Myoferlin antibody [7D2] (ab76746)")
Key features and details
- Mouse monoclonal [7D2] to Myoferlin
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
概述
-
产品名称
Anti-Myoferlin抗体[7D2]
参阅全部 Myoferlin 一抗 -
描述
小鼠单克隆抗体[7D2] to Myoferlin -
宿主
Mouse -
经测试应用
适用于: ICC/IF, Flow Cytmore details -
种属反应性
与反应: Human
预测可用于: Mouse
-
免疫原
Synthetic peptide corresponding to Human Myoferlin (N terminal).
Database link: Q9NZM1 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Constituents: 1.21% Tris, 0.75% Glycine, 2% Sucrose -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
7D2 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76746于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 5 µg/ml.
|
|
| Flow Cyt | (1) |
1/100.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
ICC/IF
Use a concentration of 5 µg/ml. |
|
Flow Cyt
1/100. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
靶标
-
功能
Calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. Involved in endocytic recycling. Implicated in VEGF signal transduction by regulating the levels of the receptor KDR. -
组织特异性
Expressed in myoblast and endothelial cells (at protein level). Highly expressed in cardiac and skeletal muscles. Also present in lung, and at very low levels in kidney, placenta and brain. -
序列相似性
Belongs to the ferlin family.
Contains 5 C2 domains. -
结构域
The C2 domain 1 associates with lipid membranes in a calcium-dependent manner. -
细胞定位
Cell membrane. Nucleus membrane. Cytoplasmic vesicle membrane. Concentrated at the membrane sites of both myoblast-myoblast and myoblast-myotube fusions. Detected at the plasmalemma in endothelial cells lining intact blood vessels (By similarity). Found at nuclear and plasma membranes. Enriched in undifferentiated myoblasts near the plasma membrane in puncate structures. - Information by UniProt
-
数据库链接
- Entrez Gene: 26509 Human
- Entrez Gene: 226101 Mouse
- Omim: 604603 Human
- SwissProt: Q9NZM1 Human
- SwissProt: Q69ZN7 Mouse
- Unigene: 602086 Human
- Unigene: 34674 Mouse
-
别名
- Fer 1 like 3, myoferlin (C. elegans) antibody
- Fer 1 like family member 3 antibody
- Fer 1 like protein 3 antibody
see all
图片
-
Immunocytochemistry/ Immunofluorescence - Anti-Myoferlin antibody [7D2] (ab76746)ICC/IF image of ab76746 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76746, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
-
![Flow Cytometry - Anti-Myoferlin antibody [7D2] (ab76746)](https://www.abcam.cn/ps/products/76/ab76746/Images/ab76746-92288-anti-myoferlin-antibody-7d2-flow-cytometry.jpg)
Flow Cytometry - Anti-Myoferlin antibody [7D2] (ab76746)Overlay histogram showing HeLa cells stained with ab76746 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76746, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (8)
ab76746 被引用在 8 文献中.
- Na JM et al. Correlation between myoferlin expression and lymph node metastasis in papillary thyroid carcinoma. J Pathol Transl Med 56:199-204 (2022). PubMed: 35535365
- Kiselev A et al. Truncating Variant in Myof Gene Is Associated With Limb-Girdle Type Muscular Dystrophy and Cardiomyopathy. Front Genet 10:608 (2019). PubMed: 31297131
- An HJ et al. Myoferlin silencing inhibits VEGFR2-mediated proliferation of metastatic clear cell renal cell carcinoma. Sci Rep 9:12656 (2019). PubMed: 31477752
- Song DH et al. Prognostic role of myoferlin expression in patients with clear cell renal cell carcinoma. Oncotarget 8:89033-89039 (2017). IHC-P ; Human . PubMed: 29179496
- Piper AK et al. Enzymatic cleavage of myoferlin releases a dual C2-domain module linked to ERK signalling. Cell Signal 33:30-40 (2017). PubMed: 28192161
- Song DH et al. Myoferlin expression in non-small cell lung cancer: Prognostic role and correlation with VEGFR-2 expression. Oncol Lett 11:998-1006 (2016). IHC . PubMed: 26893682
- Lund TC et al. sdf1 Expression reveals a source of perivascular-derived mesenchymal stem cells in zebrafish. Stem Cells 32:2767-79 (2014). Flow Cyt ; Zebrafish . PubMed: 24905975
- Leung C et al. Expression of myoferlin in human airway epithelium and its role in cell adhesion and zonula occludens-1 expression. PLoS One 7:e40478 (2012). IHC-P, WB, ICC/IF ; Human . PubMed: 22808170